ATPA_TOBAC
ID ATPA_TOBAC Reviewed; 507 AA.
AC P00823;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=ATP synthase subunit alpha, chloroplastic {ECO:0000255|HAMAP-Rule:MF_01346};
DE EC=7.1.2.2 {ECO:0000255|HAMAP-Rule:MF_01346};
DE AltName: Full=ATP synthase F1 sector subunit alpha {ECO:0000255|HAMAP-Rule:MF_01346};
DE AltName: Full=F-ATPase subunit alpha {ECO:0000255|HAMAP-Rule:MF_01346};
GN Name=atpA {ECO:0000255|HAMAP-Rule:MF_01346};
OS Nicotiana tabacum (Common tobacco).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Bright Yellow 4;
RX PubMed=6300797; DOI=10.1093/nar/11.7.2185;
RA Deno H., Shinozaki K., Sugiura M.;
RT "Nucleotide sequence of tobacco chloroplast gene for the alpha subunit of
RT proton-translocating ATPase.";
RL Nucleic Acids Res. 11:2185-2191(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Bright Yellow 4;
RX PubMed=16453699; DOI=10.1002/j.1460-2075.1986.tb04464.x;
RA Shinozaki K., Ohme M., Tanaka M., Wakasugi T., Hayashida N.,
RA Matsubayashi T., Zaita N., Chunwongse J., Obokata J.,
RA Yamaguchi-Shinozaki K., Ohto C., Torazawa K., Meng B.-Y., Sugita M.,
RA Deno H., Kamogashira T., Yamada K., Kusuda J., Takaiwa F., Kato A.,
RA Tohdoh N., Shimada H., Sugiura M.;
RT "The complete nucleotide sequence of the tobacco chloroplast genome: its
RT gene organization and expression.";
RL EMBO J. 5:2043-2049(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 463-507.
RC STRAIN=cv. Bright Yellow 4;
RX PubMed=16593407; DOI=10.1073/pnas.81.2.405;
RA Deno H., Sugiura M.;
RT "Chloroplast tRNA-Gly gene contains a long intron in the D stem: nucleotide
RT sequences of tobacco chloroplast genes for tRNA-Gly (UCC) and tRNA-Arg
RT (UCU).";
RL Proc. Natl. Acad. Sci. U.S.A. 81:405-408(1984).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
RC STRAIN=cv. Bright Yellow 4;
RA Deno H., Shinozaki K., Sugiura M.;
RT "Structure and transcription pattern of a tobacco chloroplast gene coding
RT for subunit III of proton-translocating ATPase.";
RL Gene 32:195-201(1984).
RN [5]
RP RNA EDITING.
RX PubMed=8605316; DOI=10.1007/bf00049342;
RA Hirose T., Fan H., Suzuki J.Y., Wakasugi T., Tsudzuki T., Kossel H.,
RA Sugiura M.;
RT "Occurrence of silent RNA editing in chloroplasts: its species specificity
RT and the influence of environmental and developmental conditions.";
RL Plant Mol. Biol. 30:667-672(1996).
CC -!- FUNCTION: Produces ATP from ADP in the presence of a proton gradient
CC across the membrane. The alpha chain is a regulatory subunit.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate;
CC Xref=Rhea:RHEA:57720, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.1.2.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01346};
CC -!- SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core
CC - and CF(0) - the membrane proton channel. CF(1) has five subunits:
CC alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has four main
CC subunits: a, b, b' and c. {ECO:0000255|HAMAP-Rule:MF_01346}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_01346}; Peripheral membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_01346}.
CC -!- RNA EDITING: Modified_positions=264 {ECO:0000269|PubMed:8605316};
CC -!- SIMILARITY: Belongs to the ATPase alpha/beta chains family.
CC {ECO:0000255|HAMAP-Rule:MF_01346}.
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DR EMBL; V00162; CAA23471.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z00044; CAA77341.2; ALT_SEQ; Genomic_DNA.
DR EMBL; K02396; AAA84694.1; -; Genomic_DNA.
DR EMBL; M10124; AAA84684.1; -; Genomic_DNA.
DR PIR; A01022; PWNTA.
DR RefSeq; NP_054481.2; NC_001879.2.
DR AlphaFoldDB; P00823; -.
DR SMR; P00823; -.
DR ProMEX; P00823; -.
DR GeneID; 800492; -.
DR KEGG; nta:800492; -.
DR OrthoDB; 470054at2759; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045261; C:proton-transporting ATP synthase complex, catalytic core F(1); IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046933; F:proton-transporting ATP synthase activity, rotational mechanism; IEA:UniProtKB-UniRule.
DR GO; GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:UniProtKB-EC.
DR CDD; cd18113; ATP-synt_F1_alpha_C; 1.
DR CDD; cd01132; F1_ATPase_alpha; 1.
DR Gene3D; 1.20.150.20; -; 1.
DR Gene3D; 2.40.30.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01346; ATP_synth_alpha_bact; 1.
DR InterPro; IPR023366; ATP_synth_asu-like_sf.
DR InterPro; IPR000793; ATP_synth_asu_C.
DR InterPro; IPR038376; ATP_synth_asu_C_sf.
DR InterPro; IPR033732; ATP_synth_F1_a.
DR InterPro; IPR005294; ATP_synth_F1_asu.
DR InterPro; IPR020003; ATPase_a/bsu_AS.
DR InterPro; IPR004100; ATPase_F1/V1/A1_a/bsu_N.
DR InterPro; IPR036121; ATPase_F1/V1/A1_a/bsu_N_sf.
DR InterPro; IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00006; ATP-synt_ab; 1.
DR Pfam; PF00306; ATP-synt_ab_C; 1.
DR Pfam; PF02874; ATP-synt_ab_N; 1.
DR PIRSF; PIRSF039088; F_ATPase_subunit_alpha; 1.
DR SUPFAM; SSF50615; SSF50615; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00962; atpA; 1.
DR PROSITE; PS00152; ATPASE_ALPHA_BETA; 1.
PE 2: Evidence at transcript level;
KW ATP synthesis; ATP-binding; CF(1); Chloroplast; Hydrogen ion transport;
KW Ion transport; Membrane; Nucleotide-binding; Plastid; RNA editing;
KW Thylakoid; Translocase; Transport.
FT CHAIN 1..507
FT /note="ATP synthase subunit alpha, chloroplastic"
FT /id="PRO_0000144393"
FT BINDING 170..177
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01346"
FT SITE 363
FT /note="Required for activity"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01346"
FT CONFLICT 288
FT /note="L -> P (in Ref. 2; CAA77341)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 507 AA; 55454 MW; 2A7E2C1DE2090DB9 CRC64;
MVTIRADEIS NIIRERIEQY NREVKIVNTG TVLQVGDGIA RIHGLDEVMA GELVEFEEGT
IGIALNLESN NVGVVLMGDG LLIQEGSSVK ATGRIAQIPV SEAYLGRVIN ALAKPIDGRG
EISASEFRLI ESAAPGIISR RSVYEPLQTG LIAIDSMIPI GRGQRELIIG DRQTGKTAVA
TDTILNQQGQ NVICVYVAIG QKASSVAQVV TTLQERGAME YTIVVAETAD SPATLQYLAP
YTGAALAEYF MYRERHTLII YDDLSKQAQA YRQMSLLLRR PPGREAYLGD VFYLHSRLLE
RAAKLSSSLG EGSMTALPIV ETQSGDVSAY IPTNVISITD GQIFLSADLF NSGIRPAINV
GISVSRVGSA AQIKAMKQVA GKLKLELAQF AELEAFAQFA SDLDKATQNQ LARGQRLREL
LKQSQSAPLT VEEQIMTIYT GTNGYLDSLE VGQVRKFLVE LRTYLKTNKP QFQEIISSTK
TFTEEAEALL KEAIQEQMDR FILQEQA
