RECA_PSEST
ID RECA_PSEST Reviewed; 347 AA.
AC Q07809; Q6EV35; Q6EV36;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Protein RecA {ECO:0000255|HAMAP-Rule:MF_00268};
DE AltName: Full=Recombinase A {ECO:0000255|HAMAP-Rule:MF_00268};
GN Name=recA {ECO:0000255|HAMAP-Rule:MF_00268};
OS Pseudomonas stutzeri (Pseudomonas perfectomarina).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=316;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 17587 / LMG 5838 / Stanier 220, and
RC DSM 10701 / JCM 21571 / JM300;
RA Meier P., Wackernagel W.;
RT "Cloning and sequencing of the recA gene of Pseudomonas stutzeri JM300 and
RT ATCC 17587.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.
RX PubMed=8480993; DOI=10.1007/bf00871732;
RA Vosman B., Rauch P.J., Westerhoff H.V., Hellingwerf K.J.;
RT "Regulation of the expression of the Pseudomonas stutzeri recA gene.";
RL Antonie Van Leeuwenhoek 63:55-62(1993).
CC -!- FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-
CC stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex
CC DNA, and the ATP-dependent hybridization of homologous single-stranded
CC DNAs. It interacts with LexA causing its activation and leading to its
CC autocatalytic cleavage.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00268}.
CC -!- SIMILARITY: Belongs to the RecA family. {ECO:0000255|HAMAP-
CC Rule:MF_00268}.
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DR EMBL; AJ783435; CAH03857.1; -; Genomic_DNA.
DR EMBL; AJ783436; CAH03858.1; -; Genomic_DNA.
DR EMBL; S58865; AAB26404.1; -; Genomic_DNA.
DR PIR; A56778; A56778.
DR AlphaFoldDB; Q07809; -.
DR SMR; Q07809; -.
DR STRING; 32042.PstZobell_20288; -.
DR eggNOG; COG0468; Bacteria.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR CDD; cd00983; recA; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00268; RecA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013765; DNA_recomb/repair_RecA.
DR InterPro; IPR020584; DNA_recomb/repair_RecA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR023400; RecA_C.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR PANTHER; PTHR45900; PTHR45900; 1.
DR Pfam; PF00154; RecA; 1.
DR PRINTS; PR00142; RECA.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54752; SSF54752; 1.
DR TIGRFAMs; TIGR02012; tigrfam_recA; 1.
DR PROSITE; PS00321; RECA_1; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA recombination; DNA repair;
KW DNA-binding; Nucleotide-binding; SOS response.
FT CHAIN 1..347
FT /note="Protein RecA"
FT /id="PRO_0000122809"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00268"
FT VARIANT 38
FT /note="S -> A (in strain: ATCC 17587)"
FT VARIANT 81
FT /note="A -> E (in strain: ATCC 17587)"
FT VARIANT 84
FT /note="R -> K (in strain: ATCC 17587)"
FT VARIANT 87
FT /note="S -> A (in strain: ATCC 17587)"
FT VARIANT 161
FT /note="A -> T (in strain: ATCC 17587)"
FT VARIANT 264..267
FT /note="DQAF -> GKGI (in strain: ATCC 17587)"
FT VARIANT 273
FT /note="I -> V (in strain: ATCC 17587)"
FT VARIANT 295
FT /note="S -> N (in strain: ATCC 17587)"
FT VARIANT 310
FT /note="E -> D (in strain: ATCC 17587)"
FT VARIANT 316
FT /note="Q -> R (in strain: ATCC 17587)"
FT VARIANT 324
FT /note="A -> D (in strain: ATCC 17587)"
FT VARIANT 329..339
FT /note="TSANTKATPVA -> VSGGSKANVVT (in strain: ATCC 17587)"
FT VARIANT 347
FT /note="I -> L (in strain: ATCC 17587)"
SQ SEQUENCE 347 AA; 37114 MW; 51978CB8D54CFE21 CRC64;
MDENKKRALA AALGQIEKQF GKGAVMRMGD HDRQAIPSIS TGSLGLDIAL GIGGLPKGRI
VEIYGPESSG KTTLTLSVIA AAQRMGSTCA FVDAEHALDP DYAGKLGVNV DDLLVSQPDT
GEQALEITDM LVRSNAVDVI IVDSVAALVP KAEIEGEMGD AHVGLQARLM SQALRKITGN
IKNANCLVIF INQIRMKIGV MFGSPETTTG GNALKFYASV RLDIRRTGAV KEGDEVVGSE
TRVKVVKNKV APPFRQAEFQ ILYDQAFYRN GEIIDLGVQQ GLVEKSGAWY AYKGSKIGQG
KANAAKYLEE NPEIGQEIEQ QIRAKLLVTS ANTKATPVAE DLADADI
