ID   RECF_BACSU              Reviewed;         370 AA.
AC   P05651;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 2.
DT   03-AUG-2022, entry version 146.
DE   RecName: Full=DNA replication and repair protein RecF;
GN   Name=recF; OrderedLocusNames=BSU00040;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987847; DOI=10.1093/nar/13.7.2251;
RA   Moriya S., Ogasawara N., Yoshikawa H.;
RT   "Structure and function of the region of the replication origin of the
RT   Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base
RT   pairs in the origin region.";
RL   Nucleic Acids Res. 13:2251-2265(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT   chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   SUBCELLULAR LOCATION, AND TEMPORAL ORDER OF DNA DOUBLE-STRAND BREAK
RP   RECRUITMENT.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=15186413; DOI=10.1111/j.1365-2958.2004.04102.x;
RA   Kidane D., Sanchez H., Alonso J.C., Graumann P.L.;
RT   "Visualization of DNA double-strand break repair in live bacteria reveals
RT   dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to
RT   distinct sites on the nucleoids.";
RL   Mol. Microbiol. 52:1627-1639(2004).
RN   [5]
RP   RECRUITMENT OF RECA.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=16061691; DOI=10.1083/jcb.200412090;
RA   Kidane D., Graumann P.L.;
RT   "Dynamic formation of RecA filaments at DNA double strand break repair
RT   centers in live cells.";
RL   J. Cell Biol. 170:357-366(2005).
CC   -!- FUNCTION: The RecF protein is involved in DNA metabolism; it is
CC       required for DNA replication and normal SOS inducibility. RecF binds
CC       preferentially to single-stranded, linear DNA. It also seems to bind
CC       ATP. Is recruited to repair centers, foci that are the site of double-
CC       strand DNA break(s) after RecN and RecO; recruitment may depend on
CC       RecO.
CC   -!- SUBUNIT: Recruited to foci following DNA damage; probably interacts
CC       with RecO.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid
CC       {ECO:0000269|PubMed:15186413}. Note=Cytoplasmically located in
CC       untreated cells. Recruited to foci following treatment with DNA
CC       damaging agents; these foci are presumably the breaks themselves. They
CC       are almost always located within nucleoids.
CC   -!- SIMILARITY: Belongs to the RecF family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA26220.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X02369; CAA26220.1; ALT_INIT; Genomic_DNA.
DR   EMBL; D26185; BAA05240.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11780.1; -; Genomic_DNA.
DR   PIR; D22930; D22930.
DR   RefSeq; NP_387885.1; NC_000964.3.
DR   RefSeq; WP_003243515.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P05651; -.
DR   SMR; P05651; -.
DR   IntAct; P05651; 2.
DR   STRING; 224308.BSU00040; -.
DR   PaxDb; P05651; -.
DR   PRIDE; P05651; -.
DR   DNASU; 939454; -.
DR   EnsemblBacteria; CAB11780; CAB11780; BSU_00040.
DR   GeneID; 939454; -.
DR   KEGG; bsu:BSU00040; -.
DR   PATRIC; fig|224308.179.peg.4; -.
DR   eggNOG; COG1195; Bacteria.
DR   InParanoid; P05651; -.
DR   OMA; GESWSYA; -.
DR   PhylomeDB; P05651; -.
DR   BioCyc; BSUB:BSU00040-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0043590; C:bacterial nucleoid; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0000731; P:DNA synthesis involved in DNA repair; IBA:GO_Central.
DR   GO; GO:0006302; P:double-strand break repair; IMP:UniProtKB.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.1050.90; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00365; RecF; 1.
DR   InterPro; IPR001238; DNA-binding_RecF.
DR   InterPro; IPR018078; DNA-binding_RecF_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003395; RecF/RecN/SMC_N.
DR   InterPro; IPR042174; RecF_2.
DR   PANTHER; PTHR32182:SF0; PTHR32182:SF0; 1.
DR   Pfam; PF02463; SMC_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00611; recf; 1.
DR   PROSITE; PS00617; RECF_1; 1.
DR   PROSITE; PS00618; RECF_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-binding; Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN           1..370
FT                   /note="DNA replication and repair protein RecF"
FT                   /id="PRO_0000196398"
FT   BINDING         30..37
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   370 AA;  42304 MW;  51ADB3DEA7CDE6E0 CRC64;
     MYIQNLELTS YRNYDHAELQ FENKVNVIIG ENAQGKTNLM EAIYVLSMAK SHRTSNDKEL
     IRWDKDYAKI EGRVMKQNGA IPMQLVISKK GKKGKVNHIE QQKLSQYVGA LNTIMFAPED
     LNLVKGSPQV RRRFLDMEIG QVSPVYLHDL SLYQKILSQR NHFLKQLQTR KQTDRTMLDV
     LTDQLVEVAA KVVVKRLQFT AQLEKWAQPI HAGISRGLEE LTLKYHTALD VSDPLDLSKI
     GDSYQEAFSK LREKEIERGV TLSGPHRDDV LFYVNGRDVQ TYGSQGQQRT TALSLKLAEI
     DLIHEEIGEY PILLLDDVLS ELDDYRQSHL LHTIQGRVQT FVTTTSVDGI DHETLRQAGM
     FRVQNGALVK