ID   RECF_CAUVN              Reviewed;         387 AA.
AC   B8GXP9; P49998; Q46000;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=DNA replication and repair protein RecF;
GN   Name=recF; OrderedLocusNames=CCNA_00158;
OS   Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=565050;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=NA1000 / CB15N;
RX   PubMed=9079919; DOI=10.1128/jb.179.7.2319-2330.1997;
RA   Roberts R.C., Shapiro L.;
RT   "Transcription of genes encoding DNA replication proteins is coincident
RT   with cell cycle control of DNA replication in Caulobacter crescentus.";
RL   J. Bacteriol. 179:2319-2330(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA1000 / CB15N;
RX   PubMed=20472802; DOI=10.1128/jb.00255-10;
RA   Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA   Walunas T.L., Crosson S.;
RT   "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL   J. Bacteriol. 192:3678-3688(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56.
RX   PubMed=8226640; DOI=10.1128/jb.175.21.6970-6981.1993;
RA   Rizzo M.F., Shapiro L., Gober J.;
RT   "Asymmetric expression of the gyrase B gene from the replication-competent
RT   chromosome in the Caulobacter crescentus predivisional cell.";
RL   J. Bacteriol. 175:6970-6981(1993).
CC   -!- FUNCTION: The RecF protein is involved in DNA metabolism; it is
CC       required for DNA replication and normal SOS inducibility. RecF binds
CC       preferentially to single-stranded, linear DNA. It also seems to bind
CC       ATP (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Increased susceptibility to UV light.
CC       {ECO:0000269|PubMed:9079919}.
CC   -!- SIMILARITY: Belongs to the RecF family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC43047.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U37793; AAB51449.1; -; Genomic_DNA.
DR   EMBL; CP001340; ACL93625.1; -; Genomic_DNA.
DR   EMBL; U00593; AAC43047.1; ALT_INIT; Genomic_DNA.
DR   PIR; B49347; B49347.
DR   RefSeq; WP_010918048.1; NC_011916.1.
DR   RefSeq; YP_002515533.1; NC_011916.1.
DR   AlphaFoldDB; B8GXP9; -.
DR   SMR; B8GXP9; -.
DR   PRIDE; B8GXP9; -.
DR   EnsemblBacteria; ACL93625; ACL93625; CCNA_00158.
DR   GeneID; 7332412; -.
DR   KEGG; ccs:CCNA_00158; -.
DR   PATRIC; fig|565050.3.peg.156; -.
DR   HOGENOM; CLU_040267_2_0_5; -.
DR   OMA; GESWSYA; -.
DR   OrthoDB; 891841at2; -.
DR   PhylomeDB; B8GXP9; -.
DR   Proteomes; UP000001364; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.1050.90; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00365; RecF; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR001238; DNA-binding_RecF.
DR   InterPro; IPR018078; DNA-binding_RecF_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003395; RecF/RecN/SMC_N.
DR   InterPro; IPR042174; RecF_2.
DR   PANTHER; PTHR32182:SF0; PTHR32182:SF0; 2.
DR   Pfam; PF02463; SMC_N; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00617; RECF_1; 1.
DR   PROSITE; PS00618; RECF_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-binding; Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN           1..387
FT                   /note="DNA replication and repair protein RecF"
FT                   /id="PRO_0000378304"
FT   BINDING         33..40
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        5
FT                   /note="A -> E (in Ref. 3; AAC43047)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        54..56
FT                   /note="GLR -> ACA (in Ref. 3; AAC43047)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        170..182
FT                   /note="ERMRLLVDAAETG -> RAYAPACRRRRNGR (in Ref. 1;
FT                   AAB51449)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        243
FT                   /note="L -> V (in Ref. 1; AAB51449)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   387 AA;  41172 MW;  07CA1BAE503C214A CRC64;
     MASAALLSLT LADFRSYERA RLETGGRSVY LFGANGAGKT NLLEAISLLS PGKGLRGVSL
     AEVGRRLPGE AVGRAWAVAA EVQSGEDAPV RIGTGVEQGG AARRTVRLEG ETVPPGRLAD
     HVRPIWLTPA QDRLFLEAAS ERRRFFDRLV FAGEPAHAAN ANGYDKAQRE RMRLLVDAAE
     TGAPADAAWL TALEARLAEF GALLAQARAR TLLALQAEID GRGDRPFPLA RLGLTGEWER
     MALEGAPFAE IELKLAQALA SARARDGAAG RALTGPHRGD LAIFHVEKDR PAAECSTGEQ
     KALILNLVLA QAARLSRAES APNPVILLDE VAAHLDLTRR AALADELTAL KLQAFLTGTD
     ESLFDHLKGR ALGVRVGDAG LTTLEDE