RECF_ECOLI
ID RECF_ECOLI Reviewed; 357 AA.
AC P0A7H0; P03016; Q2M812;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=DNA replication and repair protein RecF;
GN Name=recF; Synonyms=uvrF; OrderedLocusNames=b3700, JW3677;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6379647; DOI=10.1073/pnas.81.15.4622;
RA Blanar M.A., Sandler S.J., Armengod M.-E., Ream L.W., Clark A.J.;
RT "Molecular analysis of the recF gene of Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:4622-4626(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=6089112; DOI=10.1093/nar/12.16.6389;
RA Adachi T., Mizuuchi K., Menzel R., Gellert M.;
RT "DNA sequence and transcription of the region upstream of the E. coli gyrB
RT gene.";
RL Nucleic Acids Res. 12:6389-6395(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP PROTEIN SEQUENCE OF 2-13, AND DNA-BINDING.
RX PubMed=2228960; DOI=10.1128/jb.172.11.6291-6299.1990;
RA Griffin T.J. IV, Kolodner R.D.;
RT "Purification and preliminary characterization of the Escherichia coli K-12
RT recF protein.";
RL J. Bacteriol. 172:6291-6299(1990).
RN [7]
RP MUTAGENESIS OF LYS-36.
RX PubMed=1542576; DOI=10.1093/nar/20.4.839;
RA Sandler S.J., Chackerian B., Li J.T., Clark A.J.;
RT "Sequence and complementation analysis of recF genes from Escherichia coli,
RT Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence
RT for an essential phosphate binding loop.";
RL Nucleic Acids Res. 20:839-845(1992).
CC -!- FUNCTION: The RecF protein is involved in DNA metabolism; it is
CC required for DNA replication and normal SOS inducibility. RecF binds
CC preferentially to single-stranded, linear DNA. It also seems to bind
CC ATP.
CC -!- INTERACTION:
CC P0A7H0; P0A6F5: groEL; NbExp=4; IntAct=EBI-556839, EBI-543750;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RecF family. {ECO:0000305}.
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DR EMBL; K02179; AAA24511.1; -; Genomic_DNA.
DR EMBL; X04341; CAA27870.1; -; Genomic_DNA.
DR EMBL; L10328; AAA62051.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76723.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77594.1; -; Genomic_DNA.
DR PIR; A03547; RQECF.
DR RefSeq; NP_418155.1; NC_000913.3.
DR RefSeq; WP_000060112.1; NZ_STEB01000015.1.
DR AlphaFoldDB; P0A7H0; -.
DR SMR; P0A7H0; -.
DR BioGRID; 4261540; 217.
DR BioGRID; 852511; 1.
DR DIP; DIP-36037N; -.
DR IntAct; P0A7H0; 13.
DR STRING; 511145.b3700; -.
DR jPOST; P0A7H0; -.
DR PaxDb; P0A7H0; -.
DR PRIDE; P0A7H0; -.
DR EnsemblBacteria; AAC76723; AAC76723; b3700.
DR EnsemblBacteria; BAE77594; BAE77594; BAE77594.
DR GeneID; 66672402; -.
DR GeneID; 948209; -.
DR KEGG; ecj:JW3677; -.
DR KEGG; eco:b3700; -.
DR PATRIC; fig|1411691.4.peg.3003; -.
DR EchoBASE; EB0821; -.
DR eggNOG; COG1195; Bacteria.
DR HOGENOM; CLU_040267_0_0_6; -.
DR InParanoid; P0A7H0; -.
DR OMA; GESWSYA; -.
DR PhylomeDB; P0A7H0; -.
DR BioCyc; EcoCyc:EG10828-MON; -.
DR BioCyc; MetaCyc:EG10828-MON; -.
DR PRO; PR:P0A7H0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR GO; GO:0000731; P:DNA synthesis involved in DNA repair; IMP:EcoliWiki.
DR GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR GO; GO:0009314; P:response to radiation; IMP:EcoCyc.
DR GO; GO:0009411; P:response to UV; IMP:EcoliWiki.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.1050.90; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00365; RecF; 1.
DR InterPro; IPR001238; DNA-binding_RecF.
DR InterPro; IPR018078; DNA-binding_RecF_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003395; RecF/RecN/SMC_N.
DR InterPro; IPR042174; RecF_2.
DR PANTHER; PTHR32182:SF0; PTHR32182:SF0; 1.
DR Pfam; PF02463; SMC_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00611; recf; 1.
DR PROSITE; PS00617; RECF_1; 1.
DR PROSITE; PS00618; RECF_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair;
KW DNA replication; DNA-binding; Nucleotide-binding; Reference proteome;
KW SOS response.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2228960"
FT CHAIN 2..357
FT /note="DNA replication and repair protein RecF"
FT /id="PRO_0000196414"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MUTAGEN 36
FT /note="K->R: Weakly active."
FT /evidence="ECO:0000269|PubMed:1542576"
SQ SEQUENCE 357 AA; 40514 MW; ECB395B963C5DDB9 CRC64;
MSLTRLLIRD FRNIETADLA LSPGFNFLVG ANGSGKTSVL EAIYTLGHGR AFRSLQIGRV
IRHEQEAFVL HGRLQGEERE TAIGLTKDKQ GDSKVRIDGT DGHKVAELAH LMPMQLITPE
GFTLLNGGPK YRRAFLDWGC FHNEPGFFTA WSNLKRLLKQ RNAALRQVTR YEQLRPWDKE
LIPLAEQIST WRAEYSAGIA ADMADTCKQF LPEFSLTFSF QRGWEKETEY AEVLERNFER
DRQLTYTAHG PHKADLRIRA DGAPVEDTLS RGQLKLLMCA LRLAQGEFLT RESGRRCLYL
IDDFASELDD ERRGLLASRL KATQSQVFVS AISAEHVIDM SDENSKMFTV EKGKITD
