ID   RECF_LEPIN              Reviewed;         365 AA.
AC   Q8FA32; Q8GQD4;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=DNA replication and repair protein RecF {ECO:0000255|HAMAP-Rule:MF_00365};
GN   Name=recF {ECO:0000255|HAMAP-Rule:MF_00365}; OrderedLocusNames=LA_0003;
OS   Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain
OS   56601).
OC   Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX   NCBI_TaxID=189518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serogroup Pomona;
RX   PubMed=12399032; DOI=10.1111/j.1574-6968.2002.tb11388.x;
RA   Zuerner R.L., Huang W.M.;
RT   "Analysis of a Leptospira interrogans locus containing DNA replication
RT   genes and a new IS, IS1502.";
RL   FEMS Microbiol. Lett. 215:175-182(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=56601;
RX   PubMed=12712204; DOI=10.1038/nature01597;
RA   Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X.,
RA   Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X.,
RA   Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H., Yin H.-F., Zhang Y., Zhu G.-F.,
RA   Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y.,
RA   Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I.,
RA   Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P.;
RT   "Unique physiological and pathogenic features of Leptospira interrogans
RT   revealed by whole-genome sequencing.";
RL   Nature 422:888-893(2003).
CC   -!- FUNCTION: The RecF protein is involved in DNA metabolism; it is
CC       required for DNA replication and normal SOS inducibility. RecF binds
CC       preferentially to single-stranded, linear DNA. It also seems to bind
CC       ATP. {ECO:0000255|HAMAP-Rule:MF_00365}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00365}.
CC   -!- SIMILARITY: Belongs to the RecF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00365}.
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DR   EMBL; AF434658; AAN64013.1; -; Genomic_DNA.
DR   EMBL; AE010300; AAN47202.1; -; Genomic_DNA.
DR   RefSeq; NP_710184.1; NC_004342.2.
DR   RefSeq; WP_000478831.1; NC_004342.2.
DR   AlphaFoldDB; Q8FA32; -.
DR   SMR; Q8FA32; -.
DR   STRING; 189518.LA_0003; -.
DR   EnsemblBacteria; AAN47202; AAN47202; LA_0003.
DR   KEGG; lil:LA_0003; -.
DR   PATRIC; fig|189518.3.peg.7; -.
DR   HOGENOM; CLU_040267_0_1_12; -.
DR   InParanoid; Q8FA32; -.
DR   OMA; GESWSYA; -.
DR   Proteomes; UP000001408; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0000731; P:DNA synthesis involved in DNA repair; IBA:GO_Central.
DR   GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.1050.90; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00365; RecF; 1.
DR   InterPro; IPR001238; DNA-binding_RecF.
DR   InterPro; IPR018078; DNA-binding_RecF_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003395; RecF/RecN/SMC_N.
DR   InterPro; IPR042174; RecF_2.
DR   PANTHER; PTHR32182:SF0; PTHR32182:SF0; 1.
DR   Pfam; PF02463; SMC_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00611; recf; 1.
DR   PROSITE; PS00617; RECF_1; 1.
DR   PROSITE; PS00618; RECF_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-binding; Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN           1..365
FT                   /note="DNA replication and repair protein RecF"
FT                   /id="PRO_0000196427"
FT   BINDING         30..37
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00365"
FT   CONFLICT        207
FT                   /note="R -> K (in Ref. 1; AAN64013)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        240
FT                   /note="H -> R (in Ref. 1; AAN64013)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        264
FT                   /note="S -> T (in Ref. 1; AAN64013)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        363
FT                   /note="S -> P (in Ref. 1; AAN64013)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   365 AA;  42310 MW;  4485CDC76937EABD CRC64;
     MFLKHLTIQN FRNHEELSLD FDSRLIFFVG DNGEGKTNLL EAICILSWLK SFRESEDSNL
     IRWGSENYFL RGKIKDNLKE SVLEIGFTSK PSVKRKLKFN QEEIKKRTDL IGKFITVLLT
     PMDLKIIEGG PAERRKFIDA FISSFDPFYL ESLLEYNKIL KHRNALLKSG NPDISHLSIW
     DKKIVEKGIF ILNKRREVVL ELNSFYRVNL DKLSGGKDGL ELIYKPNVKD QDEFLEKLNH
     NLSRDLRLGY TSVGIHRDDL FIGSDQRDIT EFGSQGQKRS TVIALKAATF NYYKNILNTI
     PVLLIDDVIR ELDVKRREYF VDLVVTAGQA FFTTTDLEGI QDYVGKLKDQ KQIFLIRQGK
     VESIK