ID   RECF_MYCS2              Reviewed;         384 AA.
AC   A0QND8; I7F4B6; P50916;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=DNA replication and repair protein RecF;
GN   Name=recF; OrderedLocusNames=MSMEG_0003, MSMEI_0005;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 230-384.
RX   PubMed=8878580; DOI=10.1128/aac.40.9.2054;
RA   Revel-Viravau V., Truong Q.C., Moreau N., Jarlier V., Sougakoff W.;
RT   "Sequence analysis, purification, and study of inhibition by 4-quinolones
RT   of the DNA gyrase from Mycobacterium smegmatis.";
RL   Antimicrob. Agents Chemother. 40:2054-2061(1996).
CC   -!- FUNCTION: The RecF protein is involved in DNA metabolism; it is
CC       required for DNA replication and normal SOS inducibility. RecF binds
CC       preferentially to single-stranded, linear DNA. It also seems to bind
CC       ATP (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RecF family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK70100.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36490.1; -; Genomic_DNA.
DR   EMBL; X94224; CAA63915.1; -; Genomic_DNA.
DR   RefSeq; WP_011726603.1; NZ_SIJM01000001.1.
DR   RefSeq; YP_884426.1; NC_008596.1.
DR   AlphaFoldDB; A0QND8; -.
DR   SMR; A0QND8; -.
DR   STRING; 246196.MSMEI_0005; -.
DR   EnsemblBacteria; ABK70100; ABK70100; MSMEG_0003.
DR   EnsemblBacteria; AFP36490; AFP36490; MSMEI_0005.
DR   GeneID; 66738195; -.
DR   KEGG; msg:MSMEI_0005; -.
DR   KEGG; msm:MSMEG_0003; -.
DR   PATRIC; fig|246196.19.peg.3; -.
DR   eggNOG; COG1195; Bacteria.
DR   OMA; GESWSYA; -.
DR   OrthoDB; 891841at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.1050.90; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00365; RecF; 1.
DR   InterPro; IPR001238; DNA-binding_RecF.
DR   InterPro; IPR018078; DNA-binding_RecF_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003395; RecF/RecN/SMC_N.
DR   InterPro; IPR042174; RecF_2.
DR   PANTHER; PTHR32182:SF0; PTHR32182:SF0; 1.
DR   Pfam; PF02463; SMC_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00611; recf; 1.
DR   PROSITE; PS00617; RECF_1; 1.
DR   PROSITE; PS00618; RECF_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-binding; Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN           1..384
FT                   /note="DNA replication and repair protein RecF"
FT                   /id="PRO_0000293601"
FT   BINDING         30..37
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   384 AA;  41920 MW;  1F2045B42BA73262 CRC64;
     MFARHLGLTD FRSWARVDLD LEPGRTVFVG PNGFGKTNLV EALWYSATLG SHRVATDAPL
     IRAGAERAIV STIVVNEGRE LAVDLEITSG RANKARLNRS PVRSAREILG VLRAVLFSPE
     DLSLVRGDPG DRRRYLDELA TTRRPALAGV RADYDKVVRQ RTALLKTAAG ARYRGDRSVI
     DTLEVWDGHL AAHGAALVAS RVKLVEELQP EVEKAYQLLA PASRPAAIRY RSSVEAIEDA
     PGPESVEFYE AALLDALARR RDAELERGVC LVGPHRDDLE LRLGDQPAKG FASHGESWSM
     ALALRLGAYE LLCSDGVEPV LLLDDVFAEL DTSRRRALAT VAGSAEQVLV TAAVGEDIPE
     DWDARRVEIR MVEDDGGRVS MVAS