ID   RECF_MYCTU              Reviewed;         385 AA.
AC   P9WHI9; L0T293; P77896; Q59586;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=DNA replication and repair protein RecF;
GN   Name=recF; OrderedLocusNames=Rv0003; ORFNames=MTCY10H4.01;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=8733228; DOI=10.1111/j.1365-2958.1996.tb02617.x;
RA   Salazar L., Fsihi H., De Rossi E., Riccardi G., Rios C., Cole S.T.,
RA   Takiff H.E.;
RT   "Organization of the origins of replication of the chromosomes of
RT   Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium
RT   tuberculosis and isolation of a functional origin from M. smegmatis.";
RL   Mol. Microbiol. 20:283-293(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [4]
RP   SUBUNIT.
RC   STRAIN=H37Rv;
RX   PubMed=32634279; DOI=10.1111/mmi.14571;
RA   Zaveri A., Wang R., Botella L., Sharma R., Zhu L., Wallach J.B., Song N.,
RA   Jansen R.S., Rhee K.Y., Ehrt S., Schnappinger D.;
RT   "Depletion of the DarG antitoxin in Mycobacterium tuberculosis triggers the
RT   DNA-damage response and leads to cell death.";
RL   Mol. Microbiol. 114:641-652(2020).
CC   -!- FUNCTION: The RecF protein is involved in DNA metabolism; it is
CC       required for DNA replication and normal SOS inducibility. RecF binds
CC       preferentially to single-stranded, linear DNA. It also seems to bind
CC       ATP (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Co-immunoprecipitates with DarG in the presence and absence of
CC       darT. {ECO:0000269|PubMed:32634279}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RecF family. {ECO:0000305}.
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DR   EMBL; X92504; CAA63259.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP42725.1; -; Genomic_DNA.
DR   PIR; S70984; S70984.
DR   RefSeq; NP_214517.1; NC_000962.3.
DR   RefSeq; WP_003912365.1; NZ_NVQJ01000005.1.
DR   AlphaFoldDB; P9WHI9; -.
DR   SMR; P9WHI9; -.
DR   STRING; 83332.Rv0003; -.
DR   PaxDb; P9WHI9; -.
DR   DNASU; 887089; -.
DR   GeneID; 887089; -.
DR   KEGG; mtu:Rv0003; -.
DR   TubercuList; Rv0003; -.
DR   eggNOG; COG1195; Bacteria.
DR   OMA; GESWSYA; -.
DR   PhylomeDB; P9WHI9; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0000731; P:DNA synthesis involved in DNA repair; IBA:GO_Central.
DR   GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.1050.90; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00365; RecF; 1.
DR   InterPro; IPR001238; DNA-binding_RecF.
DR   InterPro; IPR018078; DNA-binding_RecF_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003395; RecF/RecN/SMC_N.
DR   InterPro; IPR042174; RecF_2.
DR   PANTHER; PTHR32182:SF0; PTHR32182:SF0; 1.
DR   Pfam; PF02463; SMC_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00611; recf; 1.
DR   PROSITE; PS00617; RECF_1; 1.
DR   PROSITE; PS00618; RECF_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-binding; Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN           1..385
FT                   /note="DNA replication and repair protein RecF"
FT                   /id="PRO_0000196435"
FT   BINDING         30..37
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   385 AA;  42212 MW;  461D6480438ADBD7 CRC64;
     MYVRHLGLRD FRSWACVDLE LHPGRTVFVG PNGYGKTNLI EALWYSTTLG SHRVSADLPL
     IRVGTDRAVI STIVVNDGRE CAVDLEIATG RVNKARLNRS SVRSTRDVVG VLRAVLFAPE
     DLGLVRGDPA DRRRYLDDLA IVRRPAIAAV RAEYERVLRQ RTALLKSVPG ARYRGDRGVF
     DTLEVWDSRL AEHGAELVAA RIDLVNQLAP EVKKAYQLLA PESRSASIGY RASMDVTGPS
     EQSDIDRQLL AARLLAALAA RRDAELERGV CLVGPHRDDL ILRLGDQPAK GFASHGEAWS
     LAVALRLAAY QLLRVDGGEP VLLLDDVFAE LDVMRRRALA TAAESAEQVL VTAAVLEDIP
     AGWDARRVHI DVRADDTGSM SVVLP