RECG_ARATH
ID RECG_ARATH Reviewed; 973 AA.
AC F4INA9; Q9ZVG0;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=ATP-dependent DNA helicase homolog RECG, chloroplastic {ECO:0000305};
DE EC=3.6.4.12 {ECO:0000305};
DE Flags: Precursor;
GN Name=RECG {ECO:0000305};
GN OrderedLocusNames=At2g01440 {ECO:0000312|Araport:AT2G01440};
GN ORFNames=F2I9.6 {ECO:0000312|EMBL:AAC67321.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- FUNCTION: Critical role in recombination and DNA repair.
CC {ECO:0000250|UniProtKB:P24230}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC67321.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005560; AAC67321.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05451.1; -; Genomic_DNA.
DR PIR; G84424; G84424.
DR RefSeq; NP_178253.3; NM_126205.4.
DR AlphaFoldDB; F4INA9; -.
DR SMR; F4INA9; -.
DR STRING; 3702.AT2G01440.1; -.
DR iPTMnet; F4INA9; -.
DR PaxDb; F4INA9; -.
DR PRIDE; F4INA9; -.
DR ProteomicsDB; 225962; -.
DR EnsemblPlants; AT2G01440.1; AT2G01440.1; AT2G01440.
DR GeneID; 814672; -.
DR Gramene; AT2G01440.1; AT2G01440.1; AT2G01440.
DR KEGG; ath:AT2G01440; -.
DR Araport; AT2G01440; -.
DR TAIR; locus:2049541; AT2G01440.
DR eggNOG; KOG0344; Eukaryota.
DR HOGENOM; CLU_005122_7_2_1; -.
DR InParanoid; F4INA9; -.
DR OMA; HQLENCG; -.
DR OrthoDB; 142915at2759; -.
DR PRO; PR:F4INA9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4INA9; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IMP:TAIR.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:TAIR.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR045562; RecG_dom3_C.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; SSF50249; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00643; recG; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Chloroplast; DNA damage; DNA recombination; DNA repair;
KW DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..24
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 25..973
FT /note="ATP-dependent DNA helicase homolog RECG,
FT chloroplastic"
FT /id="PRO_0000435302"
FT DOMAIN 536..725
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 746..904
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 174..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 655..658
FT /note="DEQQ box"
FT /evidence="ECO:0000305"
FT BINDING 549..556
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 973 AA; 108376 MW; 1103AB392F115027 CRC64;
MAAVTLSPCS MCCGSRRLRS VIVIQAQRGN WNRIRLSNFF FSKVWNISYR SKHKYSDNLL
EQVEKYASAR LENQSKLITK VAALMECDNV DDFIDKKSDE QVKKDLVLAC KRFPSIILGD
SRPVELYSNS KSYGESSSIL KTPTDNSFLP TPMHGGWFDP DNLSRTLSSF CPELLQNDDS
SDPREDILDD GSSFTSKTAT SEVEATSDDV FAAQRFLATS IDSMPGLSKR HSNQLDSCGF
HTMKKLLHHF PRTYADLQNA QVDIEDGQYL IFVGKVLSSK GVRASSSFSF LEVIVSCEVS
GRDRTPEDLS HNAEDKAGKS IFLHLKKFFR GTRFTWQPFL NSIQEKHKVG DLVCISGKVK
SLRAEDHFEM REYNIDVLKD EEESSHRAQG RPYPIYPSKG GLNPKFLSDV ISRALRVLPA
NMDPIPKEIT KVFGLPSLND AYVGIHEPKT LDEADLARKR LIFDEFFYLQ LARLYQMLQS
LGTKIEKDVL LEKFRKPVLN SVYIEEWSTL TKSFLKALPY SLTPSQLSAV SEIIWDLKRP
VPMNRLLQGD VGCGKTVVAF LACMEVIGSG YQAAFMAPTE LLAIQHYEQC RDLLENMEGV
SSKPTIGLLT GSTPAKQSRM IRQDLQSGAI SFIIGTHSLI AEKIEYSALR IAVVDEQQRF
GVIQRGKFNS KLYGTSMISK SGSSDSDDTS KADLSMAPHV LAMSATPIPR SLALALYGDI
SLTQITGMPL GRIPVETHIF EGNETGIKEV YSMMLEDLKS GGRVYVVYPV IDQSEQLPQL
RAASAELEIV TKKFPKYNCG LLHGRMKSDD KEEALNKFRS GETQILLSTQ VIEIGVDVPD
ASMMVVMNAE RFGIAQLHQL RGRVGRGTRK SKCLLVGSST NSLKRLNMLG KSSDGFYLAN
IDLLLRGPGD LLGKKQSGHL PEFPVARLEI DGNMLQEAHI AALNVLGDSH DLEKFPALKA
ELSMRQPLCL LGD
