ID   RECG_BORBU              Reviewed;         686 AA.
AC   O51528;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=ATP-dependent DNA helicase RecG;
DE            EC=3.6.4.12;
GN   Name=recG; OrderedLocusNames=BB_0581;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
CC   -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC       Holliday junction intermediates to mature products by catalyzing branch
CC       migration. Has a DNA unwinding activity characteristic of a DNA
CC       helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC       DNA) (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE000783; AAC66942.1; -; Genomic_DNA.
DR   PIR; D70172; D70172.
DR   RefSeq; NP_212715.1; NC_001318.1.
DR   RefSeq; WP_002656646.1; NC_001318.1.
DR   AlphaFoldDB; O51528; -.
DR   SMR; O51528; -.
DR   STRING; 224326.BB_0581; -.
DR   PRIDE; O51528; -.
DR   EnsemblBacteria; AAC66942; AAC66942; BB_0581.
DR   GeneID; 56568014; -.
DR   KEGG; bbu:BB_0581; -.
DR   PATRIC; fig|224326.49.peg.972; -.
DR   HOGENOM; CLU_005122_7_1_12; -.
DR   OMA; DNGFQAC; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR045562; RecG_dom3_C.
DR   InterPro; IPR033454; RecG_wedge.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF19833; RecG_C; 1.
DR   Pfam; PF17191; RecG_wedge; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00643; recG; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..686
FT                   /note="ATP-dependent DNA helicase RecG"
FT                   /id="PRO_0000102139"
FT   DOMAIN          277..438
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          457..617
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           391..394
FT                   /note="DEQH box"
FT   BINDING         290..297
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   686 AA;  79044 MW;  5D4B20F38E6BA50B CRC64;
     MFLHEFEYEL KGIGGLGEKG VERLNNLQIF NVKDLIEFFP VKYEDRQNIQ TFPDFSKVKS
     CDMMTVFTVL GHKKFGDSSK KNLKLTVKSI NEEPFEILLF NRAFLENVFK IDKKFYIYSK
     FTYNDYSGLW SCSNFDSEVY SDKPERFKKI LPVYSLTEGL TSKKISLYVK EALEYFFKFG
     QTDIPRFLIE KYSLLSLSDA LKEIHFPSSL EMLEKAKKTL IYREIFLLQF FSRYRSSKIL
     FREKKDLSKD LLEKVVSSLP FELTEDQKIS IDEIFFDLNS SKPMNRLLQG DVGSGKTLVA
     LLSGLPLIEA GYQVAFMAPT DLLARQHYDN LSNILAPFNI SMTLLTGSLR KKDKEQALES
     IRNGTSGLIV GTHAIFYEST EFKRLAYVII DEQHKFGVVQ REELKNKGEG VDMLLMSATP
     IPRSFALTLF GDLEVSFIKT LPKGRLPITT YLARHGNEDK VYEFLRKELL KGHQVYFVYP
     LISSSEKFEL KDVNNMCLKL KEVFGEYVVD MLHSKLPSDL KEEIMKNFYS KKVDILVATS
     VIEVGIDCPN ATCMVVEHAE RFGLSTLHQI RGRVGRSNLQ SFFFLLYKEP LTSAGKFRLK
     TIKENLDGFK IAEEDLRLRG PGNLFGLEQA GYLKLKIANF VDDREIIVLV REELDLFFYD
     NSAYDKLDID LLDNLFCSYL NAGRSI