RECG_HELPJ
ID RECG_HELPJ Reviewed; 623 AA.
AC Q9ZJA1;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=ATP-dependent DNA helicase RecG;
DE EC=3.6.4.12;
GN Name=recG; OrderedLocusNames=jhp_1412;
OS Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85963;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J99 / ATCC 700824;
RX PubMed=9923682; DOI=10.1038/16495;
RA Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT "Genomic sequence comparison of two unrelated isolates of the human gastric
RT pathogen Helicobacter pylori.";
RL Nature 397:176-180(1999).
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA) (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000305}.
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DR EMBL; AE001439; AAD06990.1; -; Genomic_DNA.
DR PIR; G71810; G71810.
DR RefSeq; WP_010882646.1; NC_000921.1.
DR AlphaFoldDB; Q9ZJA1; -.
DR SMR; Q9ZJA1; -.
DR STRING; 85963.jhp_1412; -.
DR PRIDE; Q9ZJA1; -.
DR EnsemblBacteria; AAD06990; AAD06990; jhp_1412.
DR KEGG; hpj:jhp_1412; -.
DR eggNOG; COG1200; Bacteria.
DR OMA; CTIQEEN; -.
DR Proteomes; UP000000804; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Helicase; Hydrolase; Nucleotide-binding.
FT CHAIN 1..623
FT /note="ATP-dependent DNA helicase RecG"
FT /id="PRO_0000102144"
FT DOMAIN 246..404
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 422..578
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 353..356
FT /note="DEQH box"
FT BINDING 259..266
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 623 AA; 71439 MW; 7C2A45B2C6CA5B3B CRC64;
MQETDDLLKT LNVKSLLEAL LVYTPKGYKD LSLLERFETG LSGVLEVGIL EKKNYAKVLK
IFAYSKRFYK NLELVFFNHS AFYYNQFKTG ESLFIYGKLE QSSFNQAYII NTPKILTEFG
KISLIFKKVK NHKKIQENLQ KLLSLENLKK EGVKENVARL LLEIFFPTPH FVKDFETNKN
FPSQHLNALK YIEMLFYMKN LERKKLQFNA KIACPNNSER LKAFIASLPF KLTRDQQNAI
KEIQSDLTSP IACKRLIIGD VGCGKTMVIL ASMVLAYPNK TLLMAPTSIL AKQLYHEALK
FLPPYFEVEL LLGGSHKKRS NHLFEKITHV VIGTQALLFD KRDLNEFALV ITDEQHRFGT
KQRYQLEKMA SSKGNKPHSL QFSATPIPRT LALAKSAFVK TTMIREIPYP KEIETLVLHK
REFKIVMEKI SEEIAKNHQV IVVYPLVNKS EKIPYLSLSE GASFWQKRFK NIYTTSGQDK
NKEEVIEEFR ELGSILLATT LIEVGISLPR LSVIVILAPE RLGLATLHQL RGRVSRNGLK
GYCFLCTIQE ENERLEKFAD ELDGFKIAEL DLQYRKSGDL LQGGKQSGNS FEYIDLARDE
NIIAEVKQDF LKNASVSQGT FEN