ID   RECG_MYCLE              Reviewed;         743 AA.
AC   O69460;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=ATP-dependent DNA helicase RecG;
DE            EC=3.6.4.12;
GN   Name=recG; OrderedLocusNames=ML1671; ORFNames=MLCB1243.01;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC       Holliday junction intermediates to mature products by catalyzing branch
CC       migration. Has a DNA unwinding activity characteristic of a DNA
CC       helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC       DNA) (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AL583923; CAC30624.1; -; Genomic_DNA.
DR   EMBL; AL023635; CAA19182.1; -; Genomic_DNA.
DR   PIR; A87118; A87118.
DR   PIR; T44697; T44697.
DR   RefSeq; NP_302148.1; NC_002677.1.
DR   RefSeq; WP_010908469.1; NC_002677.1.
DR   AlphaFoldDB; O69460; -.
DR   SMR; O69460; -.
DR   STRING; 272631.ML1671; -.
DR   EnsemblBacteria; CAC30624; CAC30624; CAC30624.
DR   KEGG; mle:ML1671; -.
DR   PATRIC; fig|272631.5.peg.3159; -.
DR   Leproma; ML1671; -.
DR   eggNOG; COG1200; Bacteria.
DR   HOGENOM; CLU_005122_7_1_11; -.
DR   OMA; DNGFQAC; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033454; RecG_wedge.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF17191; RecG_wedge; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00643; recG; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..743
FT                   /note="ATP-dependent DNA helicase RecG"
FT                   /id="PRO_0000102145"
FT   DOMAIN          302..477
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          520..694
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          524..545
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           427..430
FT                   /note="DEQH box"
FT   BINDING         315..322
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   743 AA;  81505 MW;  C1EA311C228F9205 CRC64;
     MVSLSDRLDY IVGAKAADSL DEVFGIRTVD DLLRHYPRSY TKGATVRGAQ DERPEAGEHI
     TIVDVITEAV TLPMKKDSKK KYLRLTVGSG RNKVIATFFN AGYISKGLTK DTRVMLSGEV
     GFFRGVMQLT HPAFLILDSP DGRNRGSSSL RRIADASQAV SGEVLMSAFE RRFFPIYPAS
     TKLQSWDIYA CVRQVLEVLD PVADPLPADL RAKHGLVSED EALRAIHLAE SESDRRRARE
     RLTFDEAVGL QWALVTRRHG ELSESGPSAP PRSDGLMAEL MRRLPFELTE GQREVRDVLS
     DGLAATRPLN RLLQGEVGSG KTIVAVLAML QMIDAGYQCV LLAPTEVLAA QHLLSIRDVL
     GPLGMGCQLG GAENATQVAL LTGSMTMAQK KKVRADIFSG QTGIVIGTHA LLQDAIEFHN
     LGMVVVDEQH RFGVEQRDQL RTKARTGIMP HLLVMTATPI PRTVALTVYG DLEMSTLREL
     PRGRQPITSN VIFVKDKPGW LDRAWQRILE EVAAGRQAYV VAPRIDETED PQKGGQNSRP
     SETADGLYAR LRSGELANVR LALMHGRLSA DEKDAAMMAF RAGEIDVLVC TNVIEVGVDV
     PNATIMLVMD ADRFGISQLH QLRGRIGRGT HPSLCLLASW VSPGSPAGRR LCAVAETMDG
     FALADLDLKE RREGDVLGRN QSGKAITLRM LSLAEHQVFI EAARDFCTRA YEYPHLGLAP
     HPGLADLAAR FIDTDRIEYL GKS