ID   RECG_MYCTU              Reviewed;         737 AA.
AC   P9WMQ7; L0TDY0; P64322; P95122;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=ATP-dependent DNA helicase RecG;
DE            EC=3.6.4.12;
GN   Name=recG; OrderedLocusNames=Rv2973c; ORFNames=MTCY349.14;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
CC   -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC       Holliday junction intermediates to mature products by catalyzing branch
CC       migration. Has a DNA unwinding activity characteristic of a DNA
CC       helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC       DNA) (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP45778.1; -; Genomic_DNA.
DR   PIR; B70672; B70672.
DR   RefSeq; NP_217489.1; NC_000962.3.
DR   RefSeq; WP_003415026.1; NZ_NVQJ01000015.1.
DR   AlphaFoldDB; P9WMQ7; -.
DR   SMR; P9WMQ7; -.
DR   STRING; 83332.Rv2973c; -.
DR   PaxDb; P9WMQ7; -.
DR   DNASU; 887439; -.
DR   GeneID; 887439; -.
DR   KEGG; mtu:Rv2973c; -.
DR   TubercuList; Rv2973c; -.
DR   eggNOG; COG1200; Bacteria.
DR   OMA; DNGFQAC; -.
DR   PhylomeDB; P9WMQ7; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033454; RecG_wedge.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF17191; RecG_wedge; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00643; recG; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..737
FT                   /note="ATP-dependent DNA helicase RecG"
FT                   /id="PRO_0000102146"
FT   DOMAIN          302..477
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          514..673
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           427..430
FT                   /note="DEQH box"
FT   BINDING         315..322
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   737 AA;  80361 MW;  97BAC1C8C4A0732F CRC64;
     MASLSDRLDR VLGATAADAL DEQFGMRTVD DLLRHYPRSY VEGAARVGIG DARPEAGEHI
     TIVDVITDTY SFPMKKKPNR KCLRITVGGG RNKVTATFFN ADYIMRDLTK HTKVMLSGEV
     GYYKGAMQLT HPAFLILDSP DGKNHGTRSL KSIADASKAI SGELVVEEFE RRFFPIYPAS
     TKVQSWDIFK CVRQVLDVLD RVDDPLPAEL RAKHGLIPED EALRAIHLAE SQSLRERARE
     RLTFDEAVGL QWALVARRHG ELSESGPSAA WKSNGLAAEL LRRLPFELTA GQREVLDVLS
     DGLAANRPLN RLLQGEVGSG KTIVAVLAML QMVDAGYQCA LLAPTEVLAA QHLRSIRDVL
     GPLAMGGQLG GAENATRVAL LTGSMTAGQK KQVRAEIASG QVGIVIGTHA LLQEAVDFHN
     LGMVVVDEQH RFGVEQRDQL RAKAPAGITP HLLVMTATPI PRTVALTVYG DLETSTLREL
     PLGRQPIATN VIFVKDKPAW LDRAWRRIIE EAAAGRQAYV VAPRIDESDD TDVQGGVRPS
     ATAEGLFSRL RSAELAELRL ALMHGRLSAD DKDAAMAAFR AGEVDVLVCT TVIEVGVDVP
     NATVMLVMDA DRFGISQLHQ LRGRIGRGEH PSVCLLASWV PPDTPAGQRL RAVAGTMDGF
     ALADLDLKER KEGDVLGRNQ SGKAITLRLL SLAEHEEYIV AARDFCIEAY KNPTDPALAL
     MAARFTSTDR IEYLDKS