RECG_SYNY3
ID RECG_SYNY3 Reviewed; 831 AA.
AC Q55681;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=ATP-dependent DNA helicase RecG;
DE EC=3.6.4.12;
GN Name=recG; OrderedLocusNames=slr0020;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA) (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000305}.
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DR EMBL; BA000022; BAA10207.1; -; Genomic_DNA.
DR PIR; S76355; S76355.
DR AlphaFoldDB; Q55681; -.
DR SMR; Q55681; -.
DR DIP; DIP-48810N; -.
DR IntAct; Q55681; 5.
DR STRING; 1148.1001580; -.
DR PaxDb; Q55681; -.
DR EnsemblBacteria; BAA10207; BAA10207; BAA10207.
DR KEGG; syn:slr0020; -.
DR eggNOG; COG1200; Bacteria.
DR InParanoid; Q55681; -.
DR OMA; DNGFQAC; -.
DR PhylomeDB; Q55681; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR045562; RecG_dom3_C.
DR InterPro; IPR033454; RecG_wedge.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_C; 1.
DR Pfam; PF17191; RecG_wedge; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00643; recG; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..831
FT /note="ATP-dependent DNA helicase RecG"
FT /id="PRO_0000102159"
FT DOMAIN 420..581
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 600..765
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 534..537
FT /note="DEQH box"
FT BINDING 433..440
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 831 AA; 93659 MW; F3641AB9105F4A4C CRC64;
MQCSLVEVSV SVDWPRLQKA LTVEVERGFQ NLQGKQHRFG DFLCLSFGSP PPPGSSPGDR
QKWREFAQRF AQYDQLEEAE RKSLVASTRR FLHQLRRSLE SPPRDSVPKK DLLAQVNRPQ
VSEPRYPKSG QIELHTPLAT VVSQSQHQTK LLKNLGLATV EDLLFYFPRD YLDYAQQVTI
AELTAGETVT IVGRVVNCTC FTSPKNQNLN ILQIQLRDQT GRIKLSRFYA GKRFAHRGWQ
EKIKKLYPPQ AVVAASGLVK SSKFGLTLDN PEIEVLDRHS PSIDSFKVGR VLPVYPLTEG
ITADFLRKLV LACQTAIAKL SDPLPQEIRE KYELIDLQTA IAQIHFPENT EKLSLARRRL
VFDEFFYLQL GFLQRRYEQK QQQQSAIFTP HGELLEKFSD LLPFRLTQAQ QRVVNEILQD
LNKPSPMNRL VQGDVGSGKT VVGVFAILAA LQGGYQAALM APTEVLAEQH YQKLVSWFNL
LYLPVELLTG STKTAKRREI HAQLSTGQLP LLVGTHALIQ ETVNFQRLGL VVIDEQHRFG
VQQRAKLLAK GNAPHVLSMT ATPIPRTLAL TLHGDLEVSQ IDELPPGRQP IHTSVITAKE
RPQMYELIRR EVAQGRQVYI IFPAIEESEK LDIKAAVEEH KYLTEKIFPN FNIGLLHGRL
KSAEKEAALT AFREKQTEII VSTTVIEVGV DVPNATVMVI ENAERFGLSQ LHQLRGRVGR
GSHQSYCLLV TNSKSNDARQ RLGVMEQSQD GFFIAEMDLR LRGPGEFLGT KQSGLPDFAL
ASLVEDQEVL LLAREAAETM MVEDPNLEAH PDLKIKLVQR YEKLLGGEIL T
