ID   RECJ_BACSU              Reviewed;         786 AA.
AC   O32044;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Single-stranded-DNA-specific exonuclease RecJ;
DE            EC=3.1.-.-;
GN   Name=recJ; Synonyms=yrvE; OrderedLocusNames=BSU27620;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   RECRUITS RECA TO DNA DOUBLE-STRAND BREAKS.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=16061691; DOI=10.1083/jcb.200412090;
RA   Kidane D., Graumann P.L.;
RT   "Dynamic formation of RecA filaments at DNA double strand break repair
RT   centers in live cells.";
RL   J. Cell Biol. 170:357-366(2005).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=16385024; DOI=10.1128/jb.188.2.353-360.2006;
RA   Sanchez H., Kidane D., Castillo Cozar M., Graumann P.L., Alonso J.C.;
RT   "Recruitment of Bacillus subtilis RecN to DNA double-strand breaks in the
RT   absence of DNA end processing.";
RL   J. Bacteriol. 188:353-360(2006).
CC   -!- FUNCTION: Putative single-stranded-DNA-specific exonuclease (By
CC       similarity). RecA thread formation during DNA double-strand break
CC       repair requires RecJ or AadAB. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene show only slightly
CC       increased sensitivity to DNA-damaging agents. However, cells lacking
CC       RecJ and with a mutated AadAB enzyme were extremely sensitive to these
CC       agents, as sensitive as recA deletion strains. The doubly mutated
CC       strain shows 10-fold impaired growth in the absence of these agents.
CC       {ECO:0000269|PubMed:16385024}.
CC   -!- SIMILARITY: Belongs to the RecJ family. {ECO:0000305}.
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DR   EMBL; AL009126; CAB14721.1; -; Genomic_DNA.
DR   PIR; H69980; H69980.
DR   RefSeq; NP_390640.1; NC_000964.3.
DR   RefSeq; WP_003246110.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O32044; -.
DR   SMR; O32044; -.
DR   STRING; 224308.BSU27620; -.
DR   PaxDb; O32044; -.
DR   PRIDE; O32044; -.
DR   DNASU; 937538; -.
DR   EnsemblBacteria; CAB14721; CAB14721; BSU_27620.
DR   GeneID; 937538; -.
DR   KEGG; bsu:BSU27620; -.
DR   PATRIC; fig|224308.179.peg.3001; -.
DR   eggNOG; COG0608; Bacteria.
DR   eggNOG; COG4199; Bacteria.
DR   InParanoid; O32044; -.
DR   OMA; FIICDHH; -.
DR   PhylomeDB; O32044; -.
DR   BioCyc; BSUB:BSU27620-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0008409; F:5'-3' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR   InterPro; IPR001667; DDH_dom.
DR   InterPro; IPR038763; DHH_sf.
DR   InterPro; IPR003156; DHHA1_dom.
DR   InterPro; IPR004610; RecJ.
DR   InterPro; IPR018779; RecJ_C.
DR   InterPro; IPR041122; RecJ_OB.
DR   Pfam; PF01368; DHH; 1.
DR   Pfam; PF02272; DHHA1; 1.
DR   Pfam; PF17768; RecJ_OB; 1.
DR   Pfam; PF10141; ssDNA-exonuc_C; 1.
DR   SUPFAM; SSF64182; SSF64182; 1.
DR   TIGRFAMs; TIGR00644; recJ; 1.
PE   3: Inferred from homology;
KW   Coiled coil; DNA-binding; Exonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..786
FT                   /note="Single-stranded-DNA-specific exonuclease RecJ"
FT                   /id="PRO_0000360806"
FT   DNA_BIND        485..563
FT                   /note="OB"
FT   COILED          300..332
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   786 AA;  88023 MW;  49749992D35B1229 CRC64;
     MLASKMRWEI QRPDQDKVKS LTEQLHITPL VASLLVKRGF DTAESARLFL HTKDADFYDP
     FEMKGMKEAA DRIKQAISQQ EKIMIYGDYD ADGVTSTSVM LHTLQKLSAQ VDFYIPDRFK
     EGYGPNEQAF RSIKERGFSL IITVDTGIAA VHEAKVAKEL GLDVIITDHH EPGPELPDVR
     AIVHPKQPGC TYPFKELAGV GVAFKLAHAL LGELPDELLD LAAIGTIADL VPLHDENRLI
     ATLGLERLRR TNRLGLKELI KLSGGDIGEA NEETVGFQLA PRLNAVGRIE QADPAVHLLM
     SEDSFEAEEL AAEIDQLNKE RQKMVSKMTD EAIEMVEQQG LDQTAIVVAK AGWNPGVVGI
     VASKLVDRFY RPAIVLGIDE EKGIAKGSAR SIRGFNLFES LSECRDILPH FGGHPMAAGM
     TLKAEDVPDL RSRLNEIADN TLTEEDFIPV QEVDLVCGVE DITVESIAEM NMLSPFGMLN
     PKPHVLVENA VLEDVRKIGA NKTHVKMTIR NESSQLDCVG FNKGELQEGI VPGSRISIVG
     EMSINEWNNR KKPQLMIKDA AVSEWQLFDL RGKRTWEDTV SALPSAKRAI VSFKEDSTTL
     LQTEDLRREV HVISSKDQAK AFDLDGAYIV LLDPPPSLDM LARLLEGKAP ERIYFIFLNH
     EDHFLSTFPA RDHFKWYYAF LLKRGAFDVK KHGSELAKHK GWSVETINFM TKVFFDLGFV
     KIENGVLSVV SGAKKRDLTD SQTYQAKQQL MELDQKLNYS SAEELKEWLN KLMKQDSEAY
     ESTRRT