ID   RECJ_ECOLI              Reviewed;         577 AA.
AC   P21893; Q2M9U9; Q46823;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 152.
DE   RecName: Full=Single-stranded-DNA-specific exonuclease RecJ;
DE            EC=3.1.-.-;
GN   Name=recJ; OrderedLocusNames=b2892, JW2860;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1987126; DOI=10.1128/jb.173.1.353-364.1991;
RA   Lovett S.T., Kolodner R.D.;
RT   "Nucleotide sequence of the Escherichia coli recJ chromosomal region and
RT   construction of recJ-overexpression plasmids.";
RL   J. Bacteriol. 173:353-364(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, AND PROTEIN SEQUENCE OF 1-18.
RX   PubMed=2649886; DOI=10.1073/pnas.86.8.2627;
RA   Lovett S.T., Kolodner R.D.;
RT   "Identification and purification of a single-stranded-DNA-specific
RT   exonuclease encoded by the recJ gene of Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:2627-2631(1989).
CC   -!- FUNCTION: Single-stranded-DNA-specific exonuclease. Required for many
CC       types of recombinational events, although the stringency of the
CC       requirement for RecJ appears to vary with the type of recombinational
CC       event monitored and the other recombination gene products which are
CC       available. {ECO:0000269|PubMed:2649886}.
CC   -!- INTERACTION:
CC       P21893; P14294: topB; NbExp=4; IntAct=EBI-556893, EBI-552080;
CC   -!- SIMILARITY: Belongs to the RecJ family. {ECO:0000305}.
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DR   EMBL; M54884; AAA62789.1; -; Genomic_DNA.
DR   EMBL; U28375; AAA83073.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75930.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76957.1; -; Genomic_DNA.
DR   PIR; D65073; D65073.
DR   RefSeq; NP_417368.1; NC_000913.3.
DR   RefSeq; WP_000813200.1; NZ_LN832404.1.
DR   AlphaFoldDB; P21893; -.
DR   SMR; P21893; -.
DR   BioGRID; 4262338; 178.
DR   BioGRID; 851690; 2.
DR   DIP; DIP-10654N; -.
DR   IntAct; P21893; 26.
DR   STRING; 511145.b2892; -.
DR   jPOST; P21893; -.
DR   PaxDb; P21893; -.
DR   PRIDE; P21893; -.
DR   EnsemblBacteria; AAC75930; AAC75930; b2892.
DR   EnsemblBacteria; BAE76957; BAE76957; BAE76957.
DR   GeneID; 947367; -.
DR   KEGG; ecj:JW2860; -.
DR   KEGG; eco:b2892; -.
DR   PATRIC; fig|1411691.4.peg.3841; -.
DR   EchoBASE; EB0823; -.
DR   eggNOG; COG0608; Bacteria.
DR   HOGENOM; CLU_009736_5_1_6; -.
DR   InParanoid; P21893; -.
DR   OMA; FIICDHH; -.
DR   PhylomeDB; P21893; -.
DR   BioCyc; EcoCyc:EG10830-MON; -.
DR   BioCyc; MetaCyc:EG10830-MON; -.
DR   PRO; PR:P21893; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0045145; F:single-stranded DNA 5'-3' exodeoxyribonuclease activity; IDA:EcoCyc.
DR   GO; GO:0006310; P:DNA recombination; IMP:EcoCyc.
DR   GO; GO:0006281; P:DNA repair; IMP:EcoCyc.
DR   GO; GO:0010165; P:response to X-ray; IMP:EcoCyc.
DR   InterPro; IPR001667; DDH_dom.
DR   InterPro; IPR038763; DHH_sf.
DR   InterPro; IPR003156; DHHA1_dom.
DR   InterPro; IPR004610; RecJ.
DR   InterPro; IPR041122; RecJ_OB.
DR   Pfam; PF01368; DHH; 1.
DR   Pfam; PF02272; DHHA1; 1.
DR   Pfam; PF17768; RecJ_OB; 1.
DR   SUPFAM; SSF64182; SSF64182; 1.
DR   TIGRFAMs; TIGR00644; recJ; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Exonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..577
FT                   /note="Single-stranded-DNA-specific exonuclease RecJ"
FT                   /id="PRO_0000097228"
FT   CONFLICT        6
FT                   /note="Q -> H (in Ref. 1; AAA62789)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        20
FT                   /note="A -> AA (in Ref. 1; AAA62789)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   577 AA;  63389 MW;  CF7724B36D7701DA CRC64;
     MKQQIQLRRR EVDETADLPA ELPPLLRRLY ASRGVRSAQE LERSVKGMLP WQQLSGVEKA
     VEILYNAFRE GTRIIVVGDF DADGATSTAL SVLAMRSLGC SNIDYLVPNR FEDGYGLSPE
     VVDQAHARGA QLIVTVDNGI SSHAGVEHAR SLGIPVIVTD HHLPGDTLPA AEAIINPNLR
     DCNFPSKSLA GVGVAFYLML ALRTFLRDQG WFDERNIAIP NLAELLDLVA LGTVADVVPL
     DANNRILTWQ GMSRIRAGKC RPGIKALLEV ANRDAQKLAA SDLGFALGPR LNAAGRLDDM
     SVGVALLLCD NIGEARVLAN ELDALNQTRK EIEQGMQIEA LTLCEKLERS RDTLPGGLAM
     YHPEWHQGVV GILASRIKER FHRPVIAFAP AGDGTLKGSG RSIQGLHMRD ALERLDTLYP
     GMMLKFGGHA MAAGLSLEED KFKLFQQRFG ELVTEWLDPS LLQGEVVSDG PLSPAEMTME
     VAQLLRDAGP WGQMFPEPLF DGHFRLLQQR LVGERHLKVM VEPVGGGPLL DGIAFNVDTA
     LWPDNGVREV QLAYKLDINE FRGNRSLQII IDNIWPI