ID   RECO_BACSU              Reviewed;         255 AA.
AC   P42095;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 3.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=DNA repair protein RecO;
DE   AltName: Full=Recombination protein O;
GN   Name=recO; Synonyms=yqfI, yqxN; OrderedLocusNames=BSU25280;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 122.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-114.
RC   STRAIN=168;
RA   Johnstone B.H., Torrance P.D., Maslesa-Galic S., Zarimani A.I.,
RA   Powell B.S., Court D.L., Simons R.W.;
RL   Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=10323239; DOI=10.1007/s004380051002;
RA   Fernandez S., Kobayashi Y., Ogasawara N., Alonso J.C.;
RT   "Analysis of the Bacillus subtilis recO gene: recO forms part of the
RT   RecFLOR function.";
RL   Mol. Gen. Genet. 261:567-573(1999).
RN   [6]
RP   SUBCELLULAR LOCATION, AND TEMPORAL ORDER OF DNA DOUBLE-STRAND BREAK
RP   RECRUITMENT.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=15186413; DOI=10.1111/j.1365-2958.2004.04102.x;
RA   Kidane D., Sanchez H., Alonso J.C., Graumann P.L.;
RT   "Visualization of DNA double-strand break repair in live bacteria reveals
RT   dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to
RT   distinct sites on the nucleoids.";
RL   Mol. Microbiol. 52:1627-1639(2004).
RN   [7]
RP   RECRUITMENT OF RECA.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=16061691; DOI=10.1083/jcb.200412090;
RA   Kidane D., Graumann P.L.;
RT   "Dynamic formation of RecA filaments at DNA double strand break repair
RT   centers in live cells.";
RL   J. Cell Biol. 170:357-366(2005).
CC   -!- FUNCTION: Plays a role in DNA double-stranded break repair. It seems to
CC       be involved in an RecBC-independent recombinational process of DNA
CC       repair. It may act with RecF and RecR. Is recruited to repair centers,
CC       foci that are the site of double-strand break(s) after RecN and before
CC       RecF; may actively recruit RecF.
CC   -!- SUBUNIT: Recruited to foci following DNA damage; probably interacts
CC       with RecF.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid
CC       {ECO:0000269|PubMed:15186413}. Note=Cytoplasmically located in
CC       untreated cells. Recruited to foci following treatment with DNA
CC       damaging agents; these foci are presumably the breaks themselves. They
CC       are almost always located within nucleoids.
CC   -!- SIMILARITY: Belongs to the RecO family. {ECO:0000305}.
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DR   EMBL; D84432; BAA12483.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14457.2; -; Genomic_DNA.
DR   EMBL; U18532; AAB59995.1; -; Genomic_DNA.
DR   PIR; H69968; H69968.
DR   RefSeq; NP_390406.2; NC_000964.3.
DR   RefSeq; WP_003230055.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P42095; -.
DR   SMR; P42095; -.
DR   STRING; 224308.BSU25280; -.
DR   PaxDb; P42095; -.
DR   PRIDE; P42095; -.
DR   EnsemblBacteria; CAB14457; CAB14457; BSU_25280.
DR   GeneID; 937881; -.
DR   KEGG; bsu:BSU25280; -.
DR   PATRIC; fig|224308.179.peg.2747; -.
DR   eggNOG; COG1381; Bacteria.
DR   InParanoid; P42095; -.
DR   OMA; LCTQSET; -.
DR   PhylomeDB; P42095; -.
DR   BioCyc; BSUB:BSU25280-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0043590; C:bacterial nucleoid; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006302; P:double-strand break repair; IDA:UniProtKB.
DR   Gene3D; 1.20.1440.120; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00201; RecO; 1.
DR   InterPro; IPR037278; ARFGAP/RecO.
DR   InterPro; IPR022572; DNA_rep/recomb_RecO_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR003717; RecO.
DR   InterPro; IPR042242; RecO_C.
DR   PANTHER; PTHR33991; PTHR33991; 1.
DR   Pfam; PF02565; RecO_C; 1.
DR   Pfam; PF11967; RecO_N; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF57863; SSF57863; 1.
DR   TIGRFAMs; TIGR00613; reco; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA damage; DNA recombination; DNA repair; Reference proteome.
FT   CHAIN           1..255
FT                   /note="DNA repair protein RecO"
FT                   /id="PRO_0000204932"
FT   CONFLICT        122
FT                   /note="L -> M (in Ref. 1; BAA12483)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   255 AA;  29328 MW;  01E9207DEF476276 CRC64;
     MLTKCEGIVL RTNDYGETNK IVTLLTREHG KIGVMARGAK KPNSRLSAVS QPFLYGSFLM
     QKTSGLGTLQ QGEMILSMRG IREDLFLTAY AAYVAELVDR GTEEKKPNPY LFEFILESLK
     QLNEGTDPDV ITFIVQMKML GVMGLYPELN HCVHCKSQDG TFHFSVRDNG FICHRCFEKD
     PYRIPIKPQT ARLLRLFYYF DLSRLGNVSL KEETKAELKQ VIDLYYEEYS GLYLKSKRFL
     DQMESMKHLM GENKS