RECX_ECOLI
ID RECX_ECOLI Reviewed; 166 AA.
AC P33596; P77798;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Regulatory protein RecX;
DE AltName: Full=Protein OraA;
GN Name=recX; Synonyms=oraA; OrderedLocusNames=b2698, JW2668;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / CS520;
RX PubMed=7512687; DOI=10.1016/0165-7992(94)90030-2;
RA Zaitsev E., Alexseyev A., Lanzov V., Satin L., Clark A.J.;
RT "Nucleotide sequence between recA and alaSp in E. coli K12 and the sequence
RT change in four recA mutations.";
RL Mutat. Res. 323:173-177(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION.
RX PubMed=12427742; DOI=10.1074/jbc.m210496200;
RA Stohl E.A., Brockman J.P., Burkle K.L., Morimatsu K., Kowalczykowski S.C.,
RA Seifert H.S.;
RT "Escherichia coli RecX inhibits RecA recombinase and coprotease activities
RT in vitro and in vivo.";
RL J. Biol. Chem. 278:2278-2285(2003).
RN [6]
RP SIMILARITY.
RX PubMed=8165147; DOI=10.1093/nar/22.7.1313;
RA de Mot R., Schoofs G., Vanderleyden J.;
RT "A putative regulatory gene downstream of recA is conserved in Gram-
RT negative and Gram-positive bacteria.";
RL Nucleic Acids Res. 22:1313-1314(1994).
CC -!- FUNCTION: Modulates RecA activity through direct physical interaction.
CC Can inhibit both RecA recombinase and coprotease activities. May have a
CC regulatory role during the SOS response. Inhibits DNA strand exchange
CC in vitro. {ECO:0000269|PubMed:12427742}.
CC -!- INTERACTION:
CC P33596; P61949: fldA; NbExp=3; IntAct=EBI-1129990, EBI-550021;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: By DNA damage, via LexA, as part of the SOS response.
CC -!- SIMILARITY: Belongs to the RecX family. {ECO:0000305}.
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DR EMBL; L25853; AAA19106.1; -; Unassigned_DNA.
DR EMBL; U00096; AAC75740.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16560.1; -; Genomic_DNA.
DR PIR; F65049; F65049.
DR RefSeq; NP_417178.1; NC_000913.3.
DR RefSeq; WP_000140508.1; NZ_LN832404.1.
DR AlphaFoldDB; P33596; -.
DR SMR; P33596; -.
DR BioGRID; 4259217; 129.
DR BioGRID; 851504; 1.
DR DIP; DIP-10410N; -.
DR IntAct; P33596; 5.
DR STRING; 511145.b2698; -.
DR jPOST; P33596; -.
DR PaxDb; P33596; -.
DR PRIDE; P33596; -.
DR EnsemblBacteria; AAC75740; AAC75740; b2698.
DR EnsemblBacteria; BAA16560; BAA16560; BAA16560.
DR GeneID; 947172; -.
DR KEGG; ecj:JW2668; -.
DR KEGG; eco:b2698; -.
DR PATRIC; fig|1411691.4.peg.4044; -.
DR EchoBASE; EB2005; -.
DR eggNOG; COG2137; Bacteria.
DR HOGENOM; CLU_066607_3_2_6; -.
DR InParanoid; P33596; -.
DR OMA; RCKMQEK; -.
DR PhylomeDB; P33596; -.
DR BioCyc; EcoCyc:EG12080-MON; -.
DR PRO; PR:P33596; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoCyc.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0043086; P:negative regulation of catalytic activity; IDA:EcoCyc.
DR GO; GO:0006282; P:regulation of DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEP:EcoCyc.
DR Gene3D; 1.10.10.10; -; 3.
DR HAMAP; MF_01114; RecX; 1.
DR InterPro; IPR003783; Regulatory_RecX.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR33602; PTHR33602; 1.
DR Pfam; PF02631; RecX; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA damage; DNA repair; Reference proteome; SOS response.
FT CHAIN 1..166
FT /note="Regulatory protein RecX"
FT /id="PRO_0000162429"
FT CONFLICT 19
FT /note="V -> L (in Ref. 1; AAA19106)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="L -> P (in Ref. 1; AAA19106)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 166 AA; 19424 MW; 172E4AED81712E62 CRC64;
MTESTSRRPA YARLLDRAVR ILAVRDHSEQ ELRRKLAAPI MGKNGPEEID ATAEDYERVI
AWCHEHGYLD DSRFVARFIA SRSRKGYGPA RIRQELNQKG ISREATEKAM RECDIDWCAL
ARDQATRKYG EPLPTVFSEK VKIQRFLLYR GYLMEDIQEI WRNFAD
