ID   REDF_ECOLI              Reviewed;         268 AA.
AC   P06615;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Resolvase;
DE   AltName: Full=Protein D;
GN   Name=resD; Synonyms=D; OrderedLocusNames=ECOK12F044;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3027661;
RA   Lane D., de Feyter R., Kennedy M., Phua S.-H., Semon D.;
RT   "D protein of miniF plasmid acts as a repressor of transcription and as a
RT   site-specific resolvase.";
RL   Nucleic Acids Res. 14:9713-9728(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Eichenlaub R.;
RT   "F plasmid DNA complete mini-F region (F coordinates 40.301F to 49.869F).";
RL   Submitted (AUG-1986) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 262-268.
RX   PubMed=3007930; DOI=10.1007/bf00330529;
RA   Disque-Kochem C., Seidel U., Helsberg M., Eichenlaub R.;
RT   "The repeated sequences (incB) preceding the protein E gene of plasmid
RT   mini-F are essential for replication.";
RL   Mol. Gen. Genet. 202:132-135(1986).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
RX   PubMed=6327993; DOI=10.1016/0022-2836(84)90086-x;
RA   Miki T., Yoshioka K., Horiuchi T.;
RT   "Control of cell division by sex factor F in Escherichia coli. I. The
RT   42.84-43.6 F segment couples cell division of the host bacteria with
RT   replication of plasmid DNA.";
RL   J. Mol. Biol. 174:605-625(1984).
CC   -!- FUNCTION: Acts as a repressor of transcription and as a site-specific
CC       resolvase that cleaves at the RfsF site.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA24900.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X04967; CAA28640.1; -; Genomic_DNA.
DR   EMBL; M12987; AAA24900.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP001918; BAA97914.1; -; Genomic_DNA.
DR   EMBL; X03410; CAA27145.1; -; Genomic_DNA.
DR   EMBL; X00594; CAA25245.1; -; Genomic_DNA.
DR   PIR; A24716; A24716.
DR   RefSeq; NP_061423.1; NC_002483.1.
DR   AlphaFoldDB; P06615; -.
DR   PRIDE; P06615; -.
DR   PhylomeDB; P06615; -.
DR   PRO; PR:P06615; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR016423; Resolvase_Rsv.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   PIRSF; PIRSF004576; Resolvase_Rsv; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..268
FT                   /note="Resolvase"
FT                   /id="PRO_0000197547"
FT   DOMAIN          47..250
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        82
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        114
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        202
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        205
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        228
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        237
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   CONFLICT        149
FT                   /note="Q -> T (in Ref. 3; BAA97914)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   268 AA;  29627 MW;  897C568FF4C07BBF CRC64;
     MSGSVIHSQS AVMVPAVYSA GQPASLPVAI DYPAALALRQ MSMVHDELPK YLLAPEVSAL
     LHYVPDLHRK MLLATLWNTG ARINEALALT RGDFSLAPPY PFVQLATLKQ RTEKAARTAG
     RMPAGQQTHR LVPLSDSWYV SQLQTMVAQL KIPMERRNRR TGRTEKARIW EVTDRTVRTW
     IGEAVAAAAA DGVTFSVPVT PHTFRHSYAM HMLYAGIPLK VLQSLMGHKS ISSTEVYTKV
     FALDVAARHR VQFAMPESDA VAMLKQLS