ID   REDM_ECOLX              Reviewed;         260 AA.
AC   P18021;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Resolvase;
DE   AltName: Full=Protein D;
GN   Name=resD; Synonyms=D;
OS   Escherichia coli.
OG   Plasmid ColBM-Cl139.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2853881; DOI=10.1016/0147-619x(88)90009-1;
RA   Thumm G., Oelschlaeger T., Braun V.;
RT   "Plasmid pColBM-Cl139 does not encode a colicin lysis protein but contains
RT   sequences highly homologous to the D protein (resolvase) and the oriV
RT   region of the miniF plasmid.";
RL   Plasmid 20:75-82(1988).
CC   -!- FUNCTION: This resolvase acts at the RfsF equivalent resolution
CC       sequence of pColBM-CL139.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; J03312; AAA24255.1; -; Genomic_DNA.
DR   EMBL; M35683; AAA23010.1; -; Genomic_DNA.
DR   PIR; JT0371; JT0371.
DR   RefSeq; WP_016237148.1; NZ_VRXC01000075.1.
DR   AlphaFoldDB; P18021; -.
DR   SMR; P18021; -.
DR   PATRIC; fig|562.7815.peg.4102; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR016423; Resolvase_Rsv.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   PIRSF; PIRSF004576; Resolvase_Rsv; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..260
FT                   /note="Resolvase"
FT                   /id="PRO_0000197548"
FT   DOMAIN          38..241
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        73
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        105
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        193
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        196
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        219
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        228
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   260 AA;  29177 MW;  B048CB6F1BC3FDAB CRC64;
     MQHLPAPIHH ARDAVQLPVA IDYPAALALR QMSMVHDELP KYLLAPEVSA LLHYVPDLRR
     KMLLATLWNT GARINEALAL TRGDFSLTPP YPFVQLATLK QRTEKAARTA GRMPAGQQTH
     RLVPLSDSWY VSQLQTMVAT LKIPMERRNK RTGRTEKARI WEVTDRTVRT WIGEAVAAAA
     ADGVTFSVPV TPHTFRHSYA MHMLYAGIPL KVLQSLMGHK SISSTEVYTK VFALDVAARH
     RVQFSMPESD AVTMLKNRHA