REF2P_DROME
ID REF2P_DROME Reviewed; 599 AA.
AC P14199; A6PX28; A6PX29; A6PX30; A6PX31; A6PX32; A6PX33; A6PX35; A6PX37;
AC A6PX38; A6PX44; A6PX45; A6PX46; A6PX51; A6PX56; A6PX61; A6PX72; A6PX73;
AC A6PX89; A6PX90; A6PXA2; A6PXA4; A6PXB0; A6PXB1; Q04576; Q04577; Q04578;
AC Q24063; Q24064; Q24065; Q24066; Q24067; Q24068; Q24069; Q24070; Q24071;
AC Q24072; Q541D1; Q9VIU1;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Protein ref(2)P;
DE AltName: Full=Refractory to sigma P;
GN Name=ref(2)P; ORFNames=CG10360;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP VARIANT LEU-43.
RC STRAIN=Oregon-R;
RX PubMed=2510997; DOI=10.1002/j.1460-2075.1989.tb08508.x;
RA Dezelee S., Bras F., Contamine D., Lopez-Ferber M., Segretain D.,
RA Teninges D.;
RT "Molecular analysis of ref(2)P, a Drosophila gene implicated in sigma
RT rhabdovirus multiplication and necessary for male fertility.";
RL EMBO J. 8:3437-3446(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND VARIANTS 28-GLN-ASN-29 DELINS
RP GLY; ALA-32; LEU-43; 186-LEU--ARG-192 DEL; SER-231; ARG-331;
RP 403-ASN--SER-410 DEL AND GLU-542.
RC STRAIN=Oregon-R, and Paris; TISSUE=Imaginal disk;
RX PubMed=8462852; DOI=10.1093/genetics/133.4.943;
RA Dru P., Bras F., Dezelee S., Gay P., Petitjean A.-M., Pierre-Deneubourg A.,
RA Teninges D., Contamine D.;
RT "Unusual variability of the Drosophila melanogaster ref(2)P protein which
RT controls the multiplication of sigma rhabdovirus.";
RL Genetics 133:943-954(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS 28-GLN-ASN-29 DELINS GLY;
RP ALA-32; MET-32; LEU-43; TYR-60; 186-LEU--ARG-192 DEL; SER-231; ARG-331;
RP SER-397; 403-ASN--SER-410 DEL; MET-432 AND GLU-542.
RC STRAIN=AfricaFast, AfricaSlow, Florida1Slow, Florida2Slow, FloridaFast,
RC FrenchFast, FrenchSlow, JapanFast, JapanSlow, and WashingtonFast;
RX PubMed=8583891; DOI=10.1093/oxfordjournals.molbev.a025555;
RA Wayne M.L., Contamine D., Kreitman M.;
RT "Molecular population genetics of ref(2)P, a locus which confers viral
RT resistance in Drosophila.";
RL Mol. Biol. Evol. 13:191-199(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-445, AND VARIANTS 28-GLN-ASN-29
RP DELINS GLY; ALA-32; LEU-43; TYR-60; ARG-79; SER-231; ASP-247; VAL-274;
RP ASP-290; ARG-331; LEU-356; GLU-389; LEU-393; 403-ASN--SER-410 DEL; MET-432
RP AND ILE-444.
RC STRAIN=BPL_10A, BPL_10C, BPL_10D, BPL_10E, BPL_10G, BPL_10H, BPL_11B,
RC BPL_11C, BPL_11D, BPL_11E, BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12B,
RC BPL_12C, BPL_12E, BPL_12F, BPL_13A, BPL_13B, BPL_13C, BPL_13D, BPL_13F,
RC BPL_1A, BPL_1B, BPL_1C, BPL_1D, BPL_1E, BPL_1F, BPL_1G, BPL_1H, BPL_2A,
RC BPL_2B, BPL_2C, BPL_2D, BPL_2E, BPL_2F, BPL_2G, BPL_2H, BPL_3A, BPL_3B,
RC BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_3G, BPL_3H, BPL_4A, BPL_4B, BPL_4C,
RC BPL_4D, BPL_4E, BPL_4G, BPL_4H, BPL_5A, BPL_5B, BPL_5D, BPL_5E, BPL_5F,
RC BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F, BPL_6H, BPL_7A,
RC BPL_7B, BPL_7C, BPL_7E, BPL_7F, BPL_7G, BPL_7H, BPL_8A, BPL_8B, BPL_8C,
RC BPL_8D, BPL_8E, BPL_8F, BPL_8G, BPL_8H, BPL_9A, BPL_9B, BPL_9C, BPL_9D,
RC BPL_9F, and BPL_9H;
RX PubMed=17550883; DOI=10.1098/rspb.2007.0611;
RA Bangham J., Obbard D.J., Kim K.W., Haddrill P.R., Jiggins F.M.;
RT "The age and evolution of an antiviral resistance mutation in Drosophila
RT melanogaster.";
RL Proc. R. Soc. B 274:2027-2034(2007).
RN [8]
RP FUNCTION, INTERACTION WITH APKC AND TRAF6, AND DISRUPTION PHENOTYPE.
RX PubMed=12446795; DOI=10.1128/mcb.22.24.8787-8795.2002;
RA Avila A., Silverman N., Diaz-Meco M.T., Moscat J.;
RT "The Drosophila atypical protein kinase C-ref(2)p complex constitutes a
RT conserved module for signaling in the toll pathway.";
RL Mol. Cell. Biol. 22:8787-8795(2002).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-447, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22622177; DOI=10.4161/auto.19381;
RA Taillebourg E., Gregoire I., Viargues P., Jacomin A.C., Thevenon D.,
RA Faure M., Fauvarque M.O.;
RT "The deubiquitinating enzyme USP36 controls selective autophagy activation
RT by ubiquitinated proteins.";
RL Autophagy 8:767-779(2012).
CC -!- FUNCTION: Required for selective autophagy activation by ubiquitinated
CC proteins (PubMed:22622177). Implicated in sigma rhabdovirus
CC multiplication and necessary for male fertility. Involved in activating
CC transcription of Drs (PubMed:12446795, PubMed:2510997).
CC {ECO:0000269|PubMed:12446795, ECO:0000269|PubMed:22622177,
CC ECO:0000269|PubMed:2510997}.
CC -!- SUBUNIT: Interacts with aPKC and Traf6. {ECO:0000269|PubMed:12446795}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Cytoplasm
CC {ECO:0000269|PubMed:22622177}.
CC -!- POLYMORPHISM: Polymorphic for two allelic forms in natural populations
CC of Drosophila melanogaster, ref(2)Po and ref(2)Pp. The latter allele
CC confers resistance to the rhabdovirus sigma infecting wild populations.
CC -!- DISRUPTION PHENOTYPE: Males are sterile, spermatids exhibit degenerated
CC mitochondria. Reduced activation of the Drs promoter. Double mutants
CC for ref(2)P and scny die 96 hours after egg laying (PubMed:22622177).
CC {ECO:0000269|PubMed:12446795, ECO:0000269|PubMed:22622177,
CC ECO:0000269|PubMed:2510997}.
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DR EMBL; X16993; CAA34861.1; -; Genomic_DNA.
DR EMBL; X69828; CAA49482.1; -; Genomic_DNA.
DR EMBL; X69829; CAA49483.1; -; mRNA.
DR EMBL; X69830; CAA49484.1; -; mRNA.
DR EMBL; U23923; AAA98832.1; -; Genomic_DNA.
DR EMBL; U23924; AAA98833.1; -; Genomic_DNA.
DR EMBL; U23925; AAA98834.1; -; Genomic_DNA.
DR EMBL; U23926; AAA98835.1; -; Genomic_DNA.
DR EMBL; U23927; AAA98836.1; -; Genomic_DNA.
DR EMBL; U23928; AAA98837.1; -; Genomic_DNA.
DR EMBL; U23929; AAA98838.1; -; Genomic_DNA.
DR EMBL; U23931; AAA98839.1; -; Genomic_DNA.
DR EMBL; U23932; AAA98840.1; -; Genomic_DNA.
DR EMBL; U23933; AAA98841.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF53824.1; -; Genomic_DNA.
DR EMBL; AE014134; AAX52671.1; -; Genomic_DNA.
DR EMBL; AY089494; AAL90232.1; -; mRNA.
DR EMBL; AM114104; CAJ34995.2; -; Genomic_DNA.
DR EMBL; AM114105; CAJ34996.2; -; Genomic_DNA.
DR EMBL; AM114106; CAJ34997.2; -; Genomic_DNA.
DR EMBL; AM114107; CAJ34998.2; -; Genomic_DNA.
DR EMBL; AM114108; CAJ34999.2; -; Genomic_DNA.
DR EMBL; AM114109; CAJ35000.2; -; Genomic_DNA.
DR EMBL; AM114110; CAJ35001.2; -; Genomic_DNA.
DR EMBL; AM114111; CAJ35002.2; -; Genomic_DNA.
DR EMBL; AM114112; CAJ35003.2; -; Genomic_DNA.
DR EMBL; AM114113; CAJ35004.2; -; Genomic_DNA.
DR EMBL; AM114114; CAJ35005.2; -; Genomic_DNA.
DR EMBL; AM114115; CAJ35006.2; -; Genomic_DNA.
DR EMBL; AM114116; CAJ35007.2; -; Genomic_DNA.
DR EMBL; AM114117; CAJ35008.2; -; Genomic_DNA.
DR EMBL; AM114118; CAJ35009.2; -; Genomic_DNA.
DR EMBL; AM114119; CAJ35010.2; -; Genomic_DNA.
DR EMBL; AM114120; CAJ35011.1; -; Genomic_DNA.
DR EMBL; AM114120; CAJ38290.1; -; Genomic_DNA.
DR EMBL; AM114121; CAJ35012.2; -; Genomic_DNA.
DR EMBL; AM114122; CAJ35013.2; -; Genomic_DNA.
DR EMBL; AM114123; CAJ35014.2; -; Genomic_DNA.
DR EMBL; AM114124; CAJ35015.2; -; Genomic_DNA.
DR EMBL; AM114125; CAJ35016.2; -; Genomic_DNA.
DR EMBL; AM114126; CAJ35017.2; -; Genomic_DNA.
DR EMBL; AM114127; CAJ35018.2; -; Genomic_DNA.
DR EMBL; AM114128; CAJ35019.2; -; Genomic_DNA.
DR EMBL; AM114129; CAJ35020.2; -; Genomic_DNA.
DR EMBL; AM114130; CAJ35021.2; -; Genomic_DNA.
DR EMBL; AM114131; CAJ35022.2; -; Genomic_DNA.
DR EMBL; AM114132; CAJ35023.2; -; Genomic_DNA.
DR EMBL; AM114133; CAJ35024.2; -; Genomic_DNA.
DR EMBL; AM114134; CAJ35025.2; -; Genomic_DNA.
DR EMBL; AM114135; CAJ35026.2; -; Genomic_DNA.
DR EMBL; AM114136; CAJ35027.2; -; Genomic_DNA.
DR EMBL; AM114137; CAJ35028.2; -; Genomic_DNA.
DR EMBL; AM114138; CAJ35029.2; -; Genomic_DNA.
DR EMBL; AM114139; CAJ35030.2; -; Genomic_DNA.
DR EMBL; AM114140; CAJ35031.2; -; Genomic_DNA.
DR EMBL; AM114141; CAJ35032.2; -; Genomic_DNA.
DR EMBL; AM114142; CAJ35033.2; -; Genomic_DNA.
DR EMBL; AM114143; CAJ35034.2; -; Genomic_DNA.
DR EMBL; AM114144; CAJ35035.2; -; Genomic_DNA.
DR EMBL; AM114145; CAJ35036.2; -; Genomic_DNA.
DR EMBL; AM114146; CAJ35037.2; -; Genomic_DNA.
DR EMBL; AM114147; CAJ35038.2; -; Genomic_DNA.
DR EMBL; AM114148; CAJ35039.2; -; Genomic_DNA.
DR EMBL; AM114149; CAJ35040.2; -; Genomic_DNA.
DR EMBL; AM114150; CAJ35041.2; -; Genomic_DNA.
DR EMBL; AM114151; CAJ35042.2; -; Genomic_DNA.
DR EMBL; AM114152; CAJ35043.2; -; Genomic_DNA.
DR EMBL; AM114153; CAJ35044.2; -; Genomic_DNA.
DR EMBL; AM114154; CAJ35045.2; -; Genomic_DNA.
DR EMBL; AM114155; CAJ35046.2; -; Genomic_DNA.
DR EMBL; AM114156; CAJ35047.2; -; Genomic_DNA.
DR EMBL; AM114157; CAJ35048.2; -; Genomic_DNA.
DR EMBL; AM114158; CAJ35049.2; -; Genomic_DNA.
DR EMBL; AM114159; CAJ35050.2; -; Genomic_DNA.
DR EMBL; AM114160; CAJ35051.2; -; Genomic_DNA.
DR EMBL; AM114161; CAJ35052.2; -; Genomic_DNA.
DR EMBL; AM114162; CAJ35053.2; -; Genomic_DNA.
DR EMBL; AM114163; CAJ35054.2; -; Genomic_DNA.
DR EMBL; AM114164; CAJ35055.1; -; Genomic_DNA.
DR EMBL; AM114164; CAJ38334.1; -; Genomic_DNA.
DR EMBL; AM114165; CAJ35056.2; -; Genomic_DNA.
DR EMBL; AM114166; CAJ35057.2; -; Genomic_DNA.
DR EMBL; AM114167; CAJ35058.2; -; Genomic_DNA.
DR EMBL; AM114168; CAJ35059.2; -; Genomic_DNA.
DR EMBL; AM114169; CAJ35060.2; -; Genomic_DNA.
DR EMBL; AM114170; CAJ35061.2; -; Genomic_DNA.
DR EMBL; AM114171; CAJ35062.2; -; Genomic_DNA.
DR EMBL; AM114172; CAJ35063.2; -; Genomic_DNA.
DR EMBL; AM114173; CAJ35064.2; -; Genomic_DNA.
DR EMBL; AM114174; CAJ35065.2; -; Genomic_DNA.
DR EMBL; AM114175; CAJ35066.2; -; Genomic_DNA.
DR EMBL; AM114176; CAJ35067.2; -; Genomic_DNA.
DR EMBL; AM114177; CAJ35068.2; -; Genomic_DNA.
DR EMBL; AM114178; CAJ35069.2; -; Genomic_DNA.
DR EMBL; AM114179; CAJ35070.2; -; Genomic_DNA.
DR EMBL; AM114180; CAJ35071.2; -; Genomic_DNA.
DR EMBL; AM114181; CAJ35072.2; -; Genomic_DNA.
DR EMBL; AM114182; CAJ35073.2; -; Genomic_DNA.
DR EMBL; AM114183; CAJ35074.2; -; Genomic_DNA.
DR EMBL; AM114184; CAJ35075.1; -; Genomic_DNA.
DR EMBL; AM114184; CAJ38354.1; -; Genomic_DNA.
DR EMBL; AM114185; CAJ35076.2; -; Genomic_DNA.
DR EMBL; AM114186; CAJ35077.2; -; Genomic_DNA.
DR EMBL; AM114187; CAJ35078.2; -; Genomic_DNA.
DR EMBL; AM114188; CAJ35079.1; -; Genomic_DNA.
DR EMBL; AM114188; CAJ38358.1; -; Genomic_DNA.
DR EMBL; AM114189; CAJ35080.2; -; Genomic_DNA.
DR EMBL; AM114190; CAJ35081.2; -; Genomic_DNA.
DR EMBL; AM114191; CAJ35082.2; -; Genomic_DNA.
DR PIR; S06785; S06785.
DR PIR; S33562; S29961.
DR PIR; S33563; S29963.
DR PIR; S33564; S29964.
DR RefSeq; NP_001014491.1; NM_001014491.2.
DR RefSeq; NP_476700.1; NM_057352.4.
DR AlphaFoldDB; P14199; -.
DR SMR; P14199; -.
DR BioGRID; 61223; 46.
DR DIP; DIP-18088N; -.
DR IntAct; P14199; 4.
DR MINT; P14199; -.
DR STRING; 7227.FBpp0080794; -.
DR iPTMnet; P14199; -.
DR PaxDb; P14199; -.
DR PRIDE; P14199; -.
DR DNASU; 35246; -.
DR EnsemblMetazoa; FBtr0081253; FBpp0080794; FBgn0003231.
DR EnsemblMetazoa; FBtr0100289; FBpp0099683; FBgn0003231.
DR GeneID; 35246; -.
DR KEGG; dme:Dmel_CG10360; -.
DR UCSC; CG10360-RB; d. melanogaster.
DR CTD; 35246; -.
DR FlyBase; FBgn0003231; ref(2)P.
DR VEuPathDB; VectorBase:FBgn0003231; -.
DR eggNOG; KOG4582; Eukaryota.
DR GeneTree; ENSGT00390000002781; -.
DR HOGENOM; CLU_030132_0_0_1; -.
DR InParanoid; P14199; -.
DR OMA; SIHAMMA; -.
DR OrthoDB; 1275680at2759; -.
DR PhylomeDB; P14199; -.
DR Reactome; R-DME-5205685; PINK1-PRKN Mediated Mitophagy.
DR Reactome; R-DME-9664873; Pexophagy.
DR Reactome; R-DME-9755511; KEAP1-NFE2L2 pathway.
DR BioGRID-ORCS; 35246; 0 hits in 1 CRISPR screen.
DR ChiTaRS; ref(2)P; fly.
DR GenomeRNAi; 35246; -.
DR PRO; PR:P14199; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0003231; Expressed in seminal fluid secreting gland and 57 other tissues.
DR Genevisible; P14199; DM.
DR GO; GO:0016235; C:aggresome; IBA:GO_Central.
DR GO; GO:0044753; C:amphisome; IBA:GO_Central.
DR GO; GO:0005776; C:autophagosome; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0070530; F:K63-linked polyubiquitin modification-dependent protein binding; IBA:GO_Central.
DR GO; GO:0005080; F:protein kinase C binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0035973; P:aggrephagy; IBA:GO_Central.
DR GO; GO:0007032; P:endosome organization; IBA:GO_Central.
DR GO; GO:0007005; P:mitochondrion organization; IMP:FlyBase.
DR GO; GO:0000423; P:mitophagy; IBA:GO_Central.
DR GO; GO:0061912; P:selective autophagy; IMP:UniProtKB.
DR GO; GO:0030382; P:sperm mitochondrion organization; IMP:FlyBase.
DR CDD; cd14320; UBA_SQSTM; 1.
DR Gene3D; 3.30.60.90; -; 1.
DR InterPro; IPR000270; PB1_dom.
DR InterPro; IPR033741; SQSTM_UBA.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR000433; Znf_ZZ.
DR InterPro; IPR043145; Znf_ZZ_sf.
DR Pfam; PF00564; PB1; 1.
DR Pfam; PF16577; UBA_5; 1.
DR Pfam; PF00569; ZZ; 1.
DR SMART; SM00165; UBA; 1.
DR SMART; SM00291; ZnF_ZZ; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR PROSITE; PS51745; PB1; 1.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS01357; ZF_ZZ_1; 1.
DR PROSITE; PS50135; ZF_ZZ_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Zinc; Zinc-finger.
FT CHAIN 1..599
FT /note="Protein ref(2)P"
FT /id="PRO_0000097237"
FT DOMAIN 3..88
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT REPEAT 386..393
FT /note="1"
FT REPEAT 399..406
FT /note="2"
FT REPEAT 407..413
FT /note="3"
FT DOMAIN 550..595
FT /note="UBA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT ZN_FING 122..173
FT /note="ZZ-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT REGION 95..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 184..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 245..319
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 352..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 386..413
FT /note="3 X 8 AA repeats of S-A-N-Q-S-X-X-P"
FT REGION 507..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..114
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..319
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..436
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 437..452
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..538
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 127
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 130
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 142
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 145
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 151
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 154
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 160
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 163
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT MOD_RES 447
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VARIANT 28..29
FT /note="QN -> G (in BPL_1D, BPL_2B, BPL_3E, BPL_3F, BPL_5A,
FT BPL_5D, BPL_5F, BPL_6D, BPL_6E, BPL_7F, BPL_8A, BPL_8C,
FT BPL_9H, BPL_10D, BPL_10G, BPL_11D, BPL_12B, BPL_13A,
FT BPL_13F, Florida1Slow and Paris)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:8462852, ECO:0000269|PubMed:8583891"
FT VARIANT 32
FT /note="I -> A (in strain: AfricaFast, BPL_1A, BPL_1B,
FT BPL_1D, BPL_1G, BPL_2A, BPL_2B, BPL_2C, BPL_2D, BPL_2E,
FT BPL_2G, BPL_2H, BPL_3B, BPL_3C, BPL_3D, BPL_3E, BPL_3F,
FT BPL_4A, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5D, BPL_5F,
FT BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F,
FT BPL_6H, BPL_7C, BPL_7E, BPL_7F, BPL_8A, BPL_8C, BPL_8D,
FT BPL_8E, BPL_8F, BPL_9A, BPL_9C, BPL_9F, BPL_9H, BPL_10C,
FT BPL_10D, BPL_10E, BPL_10G, BPL_11B, BPL_11C, BPL_11D,
FT BPL_11E, BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12B,
FT BPL_12C, BPL_13A, BPL_13D, BPL_13F, FloridaFast,
FT Florida1Slow, Paris and WashingtonFast)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:8462852, ECO:0000269|PubMed:8583891"
FT VARIANT 32
FT /note="I -> M (in strain: JapanSlow)"
FT /evidence="ECO:0000269|PubMed:8583891"
FT VARIANT 32
FT /note="I -> V (in strain: BPL_1D, BPL_2B, BPL_3E, BPL_3F,
FT BPL_9H, BPL_10D, BPL_10G, BPL_11D, BPL_12B and BPL_13A)"
FT VARIANT 43
FT /note="Q -> L (in strain: AfricaFast, BPL_10C, BPL_10D,
FT BPL_10E, BPL_10G, BPL_11B, BPL_11C, BPL_11D, BPL_11E,
FT BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12B, BPL_12C,
FT BPL_13A, BPL_13D, BPL_13F, BPL_1A, BPL_1B, BPL_1D, BPL_1G,
FT BPL_2A, BPL_2B, BPL_2C, BPL_2D, BPL_2E, BPL_2G, BPL_2H,
FT BPL_3A, BPL_3B, BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_4A,
FT BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5D, BPL_5F, BPL_5G,
FT BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F, BPL_6H,
FT BPL_7C, BPL_7E, BPL_7F, BPL_8A, BPL_8C, BPL_8D, BPL_8E,
FT BPL_8F, BPL_9A, BPL_9C, BPL_9F, BPL_9H, Florida1Slow,
FT FloridaFast, JapanSlow, Oregon-R, Paris and
FT WashingtonFast)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:2510997, ECO:0000269|PubMed:8462852,
FT ECO:0000269|PubMed:8583891"
FT VARIANT 60
FT /note="D -> Y (in strain: BPL_1A, BPL_1G, BPL_2A, BPL_2G,
FT BPL_2H, BPL_3C, BPL_4A, BPL_4C, BPL_4G, BPL_5H, BPL_6H,
FT BPL_7C, BPL_10E, BPL_11C, BPL_11H, BPL_12A and
FT WashingtonFast)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:8583891"
FT VARIANT 79
FT /note="C -> R (in strain: BPL_1B, BPL_9F and BPL_13D)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 186..192
FT /note="Missing (in strain: Paris)"
FT /evidence="ECO:0000269|PubMed:8462852,
FT ECO:0000269|PubMed:8583891"
FT VARIANT 231
FT /note="T -> S (in strain: BPL_10C, BPL_10D, BPL_10E,
FT BPL_10G, BPL_10H, BPL_11B, BPL_11C, BPL_11D, BPL_11F,
FT BPL_11G, BPL_11H, BPL_12A, BPL_12B, BPL_12C, BPL_13A,
FT BPL_13C, BPL_13D, BPL_13F, BPL_1A, BPL_1B, BPL_1C, BPL_1D,
FT BPL_1E, BPL_1G, BPL_1H, BPL_2A, BPL_2B, BPL_2C, BPL_2D,
FT BPL_2E, BPL_2G, BPL_2H, BPL_3A, BPL_3B, BPL_3C, BPL_3D,
FT BPL_3E, BPL_3F, BPL_3G, BPL_3H, BPL_4A, BPL_4B, BPL_4C,
FT BPL_4E, BPL_4G, BPL_5A, BPL_5D, BPL_5E, BPL_5F, BPL_5G,
FT BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F, BPL_6H,
FT BPL_7B, BPL_7C, BPL_7E, BPL_7F, BPL_7H, BPL_8A, BPL_8C,
FT BPL_8D, BPL_8E, BPL_8F, BPL_8G, BPL_9A, BPL_9C, BPL_9F,
FT BPL_9H, FrenchSlow and Paris)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:8462852, ECO:0000269|PubMed:8583891"
FT VARIANT 247
FT /note="A -> D (in strain: BPL_7A, BPL_9D, BPL_10A and
FT BPL_11E)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 274
FT /note="A -> V (in strain: BPL_1E)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 290
FT /note="V -> D (in strain: BPL_3G)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 331
FT /note="S -> R (in strain: AfricaFast, AfricaSlow, BPL_10A,
FT BPL_10C, BPL_10D, BPL_10E, BPL_10G, BPL_10H, BPL_11B,
FT BPL_11C, BPL_11D, BPL_11E, BPL_11F, BPL_11G, BPL_11H,
FT BPL_12A, BPL_12B, BPL_12C, BPL_12F, BPL_13A, BPL_13B,
FT BPL_13C, BPL_13D, BPL_13F, BPL_1A, BPL_1B, BPL_1C, BPL_1D,
FT BPL_1E, BPL_1F, BPL_1G, BPL_1H, BPL_2A, BPL_2B, BPL_2C,
FT BPL_2D, BPL_2E, BPL_2F, BPL_2G, BPL_2H, BPL_3A, BPL_3B,
FT BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_3G, BPL_3H, BPL_4A,
FT BPL_4B, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5B, BPL_5D,
FT BPL_5E, BPL_5F, BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D,
FT BPL_6E, BPL_6F, BPL_6H, BPL_7A, BPL_7B, BPL_7C, BPL_7E,
FT BPL_7F, BPL_7G, BPL_7H, BPL_8A, BPL_8B, BPL_8C, BPL_8D,
FT BPL_8E, BPL_8F, BPL_8G, BPL_8H, BPL_9A, BPL_9B, BPL_9C,
FT BPL_9D, BPL_9F, BPL_9H, Florida1Slow, Florida2Slow,
FT FloridaFast, FrenchFast, FrenchSlow, JapanFast, JapanSlow,
FT Paris and WashingtonFast)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:8462852, ECO:0000269|PubMed:8583891"
FT VARIANT 356
FT /note="S -> L (in strain: BPL_6H, BPL_7C, BPL_11F and
FT BPL_12A)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 389
FT /note="Q -> E (in strain: BPL_1C)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 393
FT /note="P -> L (in strain: BPL_1H, BPL_3H, BPL_4B, BPL_5E,
FT BPL_7B, BPL_7H, BPL_8G, BPL_10H and BPL_13C)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 397
FT /note="P -> S (in strain: FrenchSlow)"
FT /evidence="ECO:0000269|PubMed:8583891"
FT VARIANT 403..410
FT /note="Missing (in strain: AfricaFast, BPL_10C, BPL_10D,
FT BPL_10E, BPL_10G, BPL_11B, BPL_11D, BPL_11F, BPL_11G,
FT BPL_11H, BPL_12A, BPL_12C, BPL_13A, BPL_13D, BPL_13F,
FT BPL_1A, BPL_1B, BPL_1G, BPL_2A, BPL_2B, BPL_2C, BPL_2D,
FT BPL_2E, BPL_2G, BPL_2H, BPL_3A, BPL_3C, BPL_3D, BPL_3E,
FT BPL_3F, BPL_4A, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5B,
FT BPL_5D, BPL_5F, BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D,
FT BPL_6F, BPL_6H, BPL_7C, BPL_7E, BPL_7F, BPL_7G, BPL_8A,
FT BPL_8C, BPL_8D, BPL_8E, BPL_9A, BPL_9C, BPL_9F, BPL_9H,
FT Florida1Slow, Florida2Slow, FloridaFast, Paris and
FT WashingtonFast)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:8462852, ECO:0000269|PubMed:8583891"
FT VARIANT 432
FT /note="K -> M (in strain: FrenchFast)"
FT /evidence="ECO:0000269|PubMed:17550883,
FT ECO:0000269|PubMed:8583891"
FT VARIANT 444
FT /note="R -> I (in strain: BPL_3B and BPL_8F)"
FT /evidence="ECO:0000269|PubMed:17550883"
FT VARIANT 542
FT /note="D -> E (in strain: AfricaSlow, AfricaFast,
FT FloridaFast, Florida1Slow, Florida2Slow, FrenchSlow,
FT JapanSlow, Paris and WashingtonFast)"
FT /evidence="ECO:0000269|PubMed:8462852,
FT ECO:0000269|PubMed:8583891"
FT CONFLICT 188
FT /note="R -> S (in Ref. 1; CAA34861)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 599 AA; 65303 MW; 946043CE19AAF126 CRC64;
MPEKLLKITY QGAGPQKKIN AYLRMPSQNY TILRREIELY LFQERQLPKC DVRTFWIDAD
KDEIEIVNQN DYEIFLAKCE SNMHVQVAPL APVEEPKATK QEGSSANAEA PSVDDPSNFT
IHDAVECDGC GLAPLIGFRY KCVQCSNYDL CQKCELAHKH PEHLMLRMPT NNGPGMVDAW
FTGPGLGRRS GRRSRGHCPF QETNQADPAG EPARDSRRER RQARRHAGVL TQFVEMMTNL
PLNTTTATAP AEPQKPKAAE QTESPPQAEP TVTAEKAAES EAKPTEPKKV NTDQSVPRTE
DPVTTPRSTQ PTTPVINLDN ISQIVPPEYM SAGIEILNNF SEMFSKIIDT TEGGDSGIFA
PSTTPSAENK KPEEQGQSSG QSGASSANQS AVPSAAPSAN QSNVPSANQS ATPSISGSIP
DAQLETEPLN PKPSETTTET EQERRRSDSL DPEWQLIDNA YSANNSNLIN LDTTNPTAAP
QEPVRDFGQL GELLRQHMNE EARVEQASAN TQTAQVDTVS TSTSTTSVTT NSVGTSPAAP
DDKRTVPVYH TDESINKSIH AMMAMGFSNE GAWLTQLLES VQGNISAALD VMNVSQNRN
