ID   REF_BPP1                Reviewed;         186 AA.
AC   P35926;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Recombination enhancement function protein;
GN   Name=ref;
OS   Escherichia phage P1 (Bacteriophage P1).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Punavirus.
OX   NCBI_TaxID=2886926;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2542224; DOI=10.1128/jb.171.6.3427-3432.1989;
RA   Lu S.D., Lu D., Gottesman M.;
RT   "Stimulation of IS1 excision by bacteriophage P1 ref function.";
RL   J. Bacteriol. 171:3427-3432(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15489417; DOI=10.1128/jb.186.21.7032-7068.2004;
RA   Lobocka M.B., Rose D.J., Plunkett G. III, Rusin M., Samojedny A.,
RA   Lehnherr H., Yarmolinsky M.B., Blattner F.R.;
RT   "Genome of bacteriophage P1.";
RL   J. Bacteriol. 186:7032-7068(2004).
CC   -!- FUNCTION: Stimulates microhomologous recombination. Enhances the recA-
CC       dependent precise excision of an IS1 element inserted within the E.coli
CC       galT gene.
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DR   EMBL; M27041; AAA32422.1; -; Genomic_DNA.
DR   EMBL; AF234172; AAQ13980.1; -; Genomic_DNA.
DR   PIR; A60478; A60478.
DR   RefSeq; YP_006474.1; NC_005856.1.
DR   PDB; 3PLW; X-ray; 1.40 A; A=1-186.
DR   PDBsum; 3PLW; -.
DR   SMR; P35926; -.
DR   GeneID; 2777429; -.
DR   KEGG; vg:2777429; -.
DR   EvolutionaryTrace; P35926; -.
DR   Proteomes; UP000008091; Genome.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   DisProt; DP00932; -.
DR   InterPro; IPR031875; RecA_dep_nuc.
DR   Pfam; PF16786; RecA_dep_nuc; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA excision; DNA recombination; Reference proteome.
FT   CHAIN           1..186
FT                   /note="Recombination enhancement function protein"
FT                   /id="PRO_0000165280"
FT   REGION          45..81
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        45..63
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           82..92
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   HELIX           97..101
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   STRAND          109..115
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   STRAND          117..119
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   HELIX           122..124
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   STRAND          125..130
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   HELIX           131..134
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   HELIX           140..145
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   HELIX           161..167
FT                   /evidence="ECO:0007829|PDB:3PLW"
FT   HELIX           171..181
FT                   /evidence="ECO:0007829|PDB:3PLW"
SQ   SEQUENCE   186 AA;  21329 MW;  9B89AF2BB742FFC5 CRC64;
     MKTIEQKIEQ CRKWQKAARE RAIARQREKL ADPVWRESQY QKMRDTLDRR IAKQKERPPA
     SKTRKSAVKI KSRGLKGRTP TAEERRIANA LGALPCIACY MHGVISNEVS LHHIAGRTAP
     GCHKKQLPLC RWHHQHAAPA EVREKYPWLV PVHADGVVGG KKEFTLLNKS EMELLADAYE
     MANIMH