ID   REF_HEVBR               Reviewed;         138 AA.
AC   P15252;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Rubber elongation factor protein {ECO:0000303|PubMed:2808390};
DE            Short=HbREF {ECO:0000303|PubMed:2808390};
DE   AltName: Allergen=Hev b 1 {ECO:0000303|PubMed:8828541};
OS   Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Micrandreae;
OC   Hevea.
OX   NCBI_TaxID=3981;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Laticifer;
RX   PubMed=1863760; DOI=10.1007/bf00016080;
RA   Attanyaka D.P.S.T.G., Kekwick R.G.O., Franklin F.C.H.;
RT   "Molecular cloning and nucleotide sequencing of the rubber elongation
RT   factor gene from Hevea brasiliensis.";
RL   Plant Mol. Biol. 16:1079-1081(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-138, AND ACETYLATION AT ALA-2.
RX   PubMed=2808390; DOI=10.1016/s0021-9258(18)51511-8;
RA   Dennis M.S., Henzel W.J., Kohr W., Light D.R.;
RT   "Amino acid sequence of rubber elongation factor protein associated with
RT   rubber particles in Hevea latex.";
RL   J. Biol. Chem. 264:18618-18626(1989).
RN   [3]
RP   ALLERGEN.
RX   PubMed=8828541; DOI=10.1016/s0091-6749(96)70097-0;
RA   Yeang H.Y., Cheong K.F., Sunderasan E., Hamzah S., Chew N.P., Hamid S.,
RA   Hamilton R.G., Cardosa M.J.;
RT   "The 14.6 kd rubber elongation factor (Hev b 1) and 24 kd (Hev b 3) rubber
RT   particle proteins are recognized by IgE from patients with spina bifida and
RT   latex allergy.";
RL   J. Allergy Clin. Immunol. 98:628-639(1996).
RN   [4]
RP   EPITOPE MAPPING.
RX   PubMed=11275264; DOI=10.1016/s0161-5890(00)00095-x;
RA   Banerjee B., Kanitpong K., Fink J.N., Zussman M., Sussman G.L., Kelly K.J.,
RA   Kurup V.P.;
RT   "Unique and shared IgE epitopes of Hev b 1 and Hev b 3 in latex allergy.";
RL   Mol. Immunol. 37:789-798(2000).
RN   [5]
RP   INDUCTION.
RX   PubMed=12461132; DOI=10.1093/pcp/pcf161;
RA   Sookmark U., Pujade-Renaud V., Chrestin H., Lacote R., Naiyanetr C.,
RA   Seguin M., Romruensukharom P., Narangajavana J.;
RT   "Characterization of polypeptides accumulated in the latex cytosol of
RT   rubber trees affected by the tapping panel dryness syndrome.";
RL   Plant Cell Physiol. 43:1323-1333(2002).
RN   [6]
RP   INDUCTION BY TAPPING AND ETHEPHON.
RX   PubMed=17632724; DOI=10.1007/s00299-007-0402-z;
RA   Priya P., Venkatachalam P., Thulaseedharan A.;
RT   "Differential expression pattern of rubber elongation factor (REF) mRNA
RT   transcripts from high and low yielding clones of rubber tree (Hevea
RT   brasiliensis Muell. Arg.).";
RL   Plant Cell Rep. 26:1833-1838(2007).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   PubMed=19415323; DOI=10.1007/s00425-009-0936-0;
RA   Sando T., Hayashi T., Takeda T., Akiyama Y., Nakazawa Y., Fukusaki E.,
RA   Kobayashi A.;
RT   "Histochemical study of detailed laticifer structure and rubber
RT   biosynthesis-related protein localization in Hevea brasiliensis using
RT   spectral confocal laser scanning microscopy.";
RL   Planta 230:215-225(2009).
RN   [8]
RP   INDUCTION BY ETHYLENE.
RX   PubMed=22162870; DOI=10.1093/jxb/err363;
RA   Chow K.S., Mat-Isa M.N., Bahari A., Ghazali A.K., Alias H.,
RA   Mohd-Zainuddin Z., Hoh C.C., Wan K.L.;
RT   "Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis
RT   latex.";
RL   J. Exp. Bot. 63:1863-1871(2012).
RN   [9]
RP   SUBCELLULAR LOCATION, AND SUBUNIT.
RX   PubMed=24036080; DOI=10.1016/j.bbamem.2013.08.025;
RA   Berthelot K., Lecomte S., Estevez Y., Zhendre V., Henry S., Thevenot J.,
RA   Dufourc E.J., Alves I.D., Peruch F.;
RT   "Rubber particle proteins, HbREF and HbSRPP, show different interactions
RT   with model membranes.";
RL   Biochim. Biophys. Acta 1838:287-299(2014).
RN   [10]
RP   SUBUNIT, AND LACK OF GLYCOSYLATION.
RX   PubMed=24239687; DOI=10.1016/j.bbapap.2013.10.017;
RA   Berthelot K., Lecomte S., Estevez Y., Coulary-Salin B., Peruch F.;
RT   "Homologous Hevea brasiliensis REF (Hevb1) and SRPP (Hevb3) present
RT   different auto-assembling.";
RL   Biochim. Biophys. Acta 1844:473-485(2014).
RN   [11]
RP   REVIEW.
RX   PubMed=25019490; DOI=10.1016/j.biochi.2014.07.002;
RA   Berthelot K., Lecomte S., Estevez Y., Peruch F.;
RT   "Hevea brasiliensis REF (Hev b 1) and SRPP (Hev b 3): An overview on rubber
RT   particle proteins.";
RL   Biochimie 106:1-9(2014).
CC   -!- FUNCTION: May be part of the rubber biosynthesis machinery. Plays a
CC       role in rubber elongation. {ECO:0000305|PubMed:1863760}.
CC   -!- SUBUNIT: In solution, able to form amyloid fibers and aggregates rich
CC       in beta-sheets (PubMed:24239687). Interaction with membrane stabilizes
CC       the protein, inhibiting the amyloid state and aggregation
CC       (PubMed:24036080). {ECO:0000269|PubMed:24036080,
CC       ECO:0000269|PubMed:24239687}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24036080}.
CC       Note=Tightly bound on latex large rubber particles. Binds first to
CC       various type of lipids and then inserts into the membrane as protein
CC       rafts. {ECO:0000269|PubMed:24036080}.
CC   -!- TISSUE SPECIFICITY: Localized in all laticifer layers.
CC       {ECO:0000269|PubMed:19415323}.
CC   -!- INDUCTION: Induced by tapping (PubMed:12461132, PubMed:17632724). Not
CC       induced by wounding, ethephon or abscisic acid treatment
CC       (PubMed:12461132). Induced by ethephon or ethylene (PubMed:17632724,
CC       PubMed:22162870). {ECO:0000269|PubMed:12461132,
CC       ECO:0000269|PubMed:17632724, ECO:0000269|PubMed:22162870}.
CC   -!- PTM: Not glycosylated. {ECO:0000269|PubMed:24239687}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Involved in latex
CC       allergic reactions. {ECO:0000269|PubMed:8828541}.
CC   -!- SIMILARITY: Belongs to the REF/SRPP family. {ECO:0000305}.
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DR   EMBL; X56535; CAA39880.1; -; mRNA.
DR   PIR; S16258; A34309.
DR   AlphaFoldDB; P15252; -.
DR   Allergome; 3310; Hev b 1.0101.
DR   Allergome; 379; Hev b 1.
DR   iPTMnet; P15252; -.
DR   PRIDE; P15252; -.
DR   OrthoDB; 1309723at2759; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008802; REF.
DR   PANTHER; PTHR33732; PTHR33732; 1.
DR   Pfam; PF05755; REF; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Allergen; Cytoplasm; Direct protein sequencing.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2808390"
FT   CHAIN           2..138
FT                   /note="Rubber elongation factor protein"
FT                   /id="PRO_0000221060"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000269|PubMed:2808390"
SQ   SEQUENCE   138 AA;  14722 MW;  BE37206378306306 CRC64;
     MAEDEDNQQG QGEGLKYLGF VQDAATYAVT TFSNVYLFAK DKSGPLQPGV DIIEGPVKNV
     AVPLYNRFSY IPNGALKFVD STVVASVTII DRSLPPIVKD ASIQVVSAIR AAPEAARSLA
     SSLPGQTKIL AKVFYGEN