ID   REGA_BPR69              Reviewed;         122 AA.
AC   Q01751;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   12-AUG-2020, entry version 63.
DE   RecName: Full=Translation repressor protein;
GN   Name=regA;
OS   Escherichia phage RB69 (Bacteriophage RB69).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Mosigvirus.
OX   NCBI_TaxID=12353;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1594613; DOI=10.1073/pnas.89.11.5053;
RA   Jozwik C.E., Miller E.S.;
RT   "Regions of bacteriophage T4 and RB69 RegA translational repressor proteins
RT   that determine RNA-binding specificity.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:5053-5057(1992).
CC   -!- FUNCTION: Controls the translation of a number of proteins (such as
CC       regA itself, rIIB and at least 35 others) by binding to their mRNA.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M86231; AAA32295.1; -; Genomic_DNA.
DR   PIR; A45385; A45385.
DR   SMR; Q01751; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.650; -; 1.
DR   InterPro; IPR002702; Transl_repress_RegA.
DR   InterPro; IPR036516; Transl_repress_RegA_sf.
DR   Pfam; PF01818; Translat_reg; 1.
DR   SUPFAM; SSF55064; SSF55064; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Repressor; Translation regulation.
FT   CHAIN           1..122
FT                   /note="Translation repressor protein"
FT                   /id="PRO_0000164972"
FT   DNA_BIND        15..37
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         24
FT                   /note="I->T: Activity altered."
FT   MUTAGEN         25
FT                   /note="A->V: 100% activity loss."
FT   MUTAGEN         37
FT                   /note="H->Y: 100% activity loss."
FT   MUTAGEN         72
FT                   /note="D->G: 100% activity loss."
SQ   SEQUENCE   122 AA;  14432 MW;  C4374CE90E16D1D3 CRC64;
     MIEIKLKNPE DFLKVKETLT RMGIANNKDK VLYQSCHILQ KQGKYYIVHF KEMLRMDGRQ
     VDIDGEDYQR RDSIAQLLED WGLIVIEDSA REDLFGLTNN FRVISFKQKD DWTLKAKYTI
     GN