ID   REGA_BPT4               Reviewed;         122 AA.
AC   P69702; P04528;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Translation repressor protein;
GN   Name=regA;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6473098; DOI=10.1093/nar/12.15.5979;
RA   Trojanowska M., Miller E.S., Karam J., Stormo G., Gold L.;
RT   "The bacteriophage T4 regA gene: primary sequence of a translational
RT   repressor.";
RL   Nucleic Acids Res. 12:5979-5993(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3872458; DOI=10.1073/pnas.82.7.1901;
RA   Adari H.Y., Rose K., Williams K.R., Konigsberg W.H., Lin T.C., Spicer E.K.;
RT   "Cloning, nucleotide sequence, and overexpression of the bacteriophage T4
RT   regA gene.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:1901-1905(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [4]
RP   MECHANISM OF TRANSLATION REGULATION.
RX   PubMed=3120177; DOI=10.1073/pnas.84.22.7822;
RA   Winter R.B., Morrisey L., Gauss P., Gold L., Hsu T., Karam J.;
RT   "Bacteriophage T4 regA protein binds to mRNAs and prevents translation
RT   initiation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:7822-7826(1987).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=1464621; DOI=10.1016/s0021-9258(18)35722-3;
RA   Webster K.R., Keill S., Konigsberg W., Williams K.R., Spicer E.K.;
RT   "Identification of amino acid residues at the interface of a bacteriophage
RT   T4 regA protein-nucleic acid complex.";
RL   J. Biol. Chem. 267:26097-26103(1992).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
RX   PubMed=7761833; DOI=10.1126/science.7761833;
RA   Kang C., Chan R., Berger I., Lockshin C., Green L., Gold L., Rich A.;
RT   "Crystal structure of the T4 regA translational regulator protein at 1.9-A
RT   resolution.";
RL   Science 268:1170-1173(1995).
CC   -!- FUNCTION: Controls the translation of a number of proteins (such as
CC       regA itself, rIIB and at least 35 others) by binding to their mRNA.
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DR   EMBL; X00769; CAA25343.1; -; Genomic_DNA.
DR   EMBL; M10160; AAC05396.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42651.1; -; Genomic_DNA.
DR   PIR; A93530; RGBPT4.
DR   RefSeq; NP_049663.1; NC_000866.4.
DR   PDB; 1REG; X-ray; 1.90 A; X/Y=1-122.
DR   PDBsum; 1REG; -.
DR   SMR; P69702; -.
DR   GeneID; 1258694; -.
DR   KEGG; vg:1258694; -.
DR   EvolutionaryTrace; P69702; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.650; -; 1.
DR   InterPro; IPR002702; Transl_repress_RegA.
DR   InterPro; IPR036516; Transl_repress_RegA_sf.
DR   Pfam; PF01818; Translat_reg; 1.
DR   SUPFAM; SSF55064; SSF55064; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Repressor; RNA-binding;
KW   Translation regulation.
FT   CHAIN           1..122
FT                   /note="Translation repressor protein"
FT                   /id="PRO_0000164968"
FT   DNA_BIND        15..37
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   STRAND          2..5
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   HELIX           11..20
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   STRAND          22..26
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   TURN            27..30
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   STRAND          31..34
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   STRAND          36..41
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   STRAND          44..49
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   HELIX           50..56
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   HELIX           65..80
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   TURN            106..108
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   HELIX           109..111
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   STRAND          112..116
FT                   /evidence="ECO:0007829|PDB:1REG"
FT   TURN            117..119
FT                   /evidence="ECO:0007829|PDB:1REG"
SQ   SEQUENCE   122 AA;  14619 MW;  616DD4E11D70EC07 CRC64;
     MIEITLKKPE DFLKVKETLT RMGIANNKDK VLYQSCHILQ KKGLYYIVHF KEMLRMDGRQ
     VEMTEEDEVR RDSIAWLLED WGLIEIVPGQ RTFMKDLTNN FRVISFKQKH EWKLVPKYTI
     GN