REGX3_MYCS2
ID REGX3_MYCS2 Reviewed; 228 AA.
AC Q9F868; A0QR02; I7G4G1;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Sensory transduction protein RegX3;
GN Name=regX3; OrderedLocusNames=MSMEG_0937, MSMEI_0914;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Harris R.H., Benjamin W.H. Jr., Briles D.E., Dunlap N.E.;
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [5]
RP FUNCTION IN REGULATION OF PHOSPHATE-DEPENDENT GENE EXPRESSION.
RX PubMed=17526710; DOI=10.1128/jb.00190-07;
RA Glover R.T., Kriakov J., Garforth S.J., Baughn A.D., Jacobs W.R. Jr.;
RT "The two-component regulatory system senX3-regX3 regulates phosphate-
RT dependent gene expression in Mycobacterium smegmatis.";
RL J. Bacteriol. 189:5495-5503(2007).
RN [6]
RP REGULATION OF PHNDCE OPERON, AND INDUCTION.
RX PubMed=18083811; DOI=10.1128/jb.01764-07;
RA Gebhard S., Cook G.M.;
RT "Differential regulation of high-affinity phosphate transport systems of
RT Mycobacterium smegmatis: identification of PhnF, a repressor of the phnDCE
RT operon.";
RL J. Bacteriol. 190:1335-1343(2008).
CC -!- FUNCTION: Part of the two-component regulatory system SenX3/RegX3. Once
CC phosphorylated by SenX3, activates the expression of several
CC operons/genes involved in phosphate assimilation and metabolism, such
CC as pstSCAB, phnDCE, and phoA. {ECO:0000269|PubMed:17526710}.
CC -!- INDUCTION: By phosphate-limiting growth conditions.
CC {ECO:0000269|PubMed:18083811}.
CC -!- PTM: Phosphorylated by SenX3. {ECO:0000250}.
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DR EMBL; AF258346; AAG09797.1; -; Genomic_DNA.
DR EMBL; CP000480; ABK72892.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP37394.1; -; Genomic_DNA.
DR RefSeq; WP_014876914.1; NZ_SIJM01000010.1.
DR RefSeq; YP_885340.1; NC_008596.1.
DR AlphaFoldDB; Q9F868; -.
DR SMR; Q9F868; -.
DR STRING; 246196.MSMEI_0914; -.
DR EnsemblBacteria; ABK72892; ABK72892; MSMEG_0937.
DR EnsemblBacteria; AFP37394; AFP37394; MSMEI_0914.
DR GeneID; 66739104; -.
DR KEGG; msg:MSMEI_0914; -.
DR KEGG; msm:MSMEG_0937; -.
DR PATRIC; fig|246196.19.peg.927; -.
DR eggNOG; COG0745; Bacteria.
DR OrthoDB; 817710at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..228
FT /note="Sensory transduction protein RegX3"
FT /id="PRO_0000081333"
FT DOMAIN 3..116
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 129..228
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 52
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 228 AA; 24911 MW; 6DCE8D7021CA5B00 CRC64;
MTSVLIVEDE ESLADPLAFL LRKEGFEATV VGDGPSALAE FERSGADIVL LDLMLPGMSG
TDVCKQLRAR SSVPVIMVTA RDSEIDKVVG LELGADDYVT KPYSARELIA RIRAVLRRGA
DNDDAGADDG VLEAGPVRMD VERHVVSVNG EPITLPLKEF DLLEYLMRNS GRVLTRGQLI
DRVWGADYVG DTKTLDVHVK RLRSKIEEDP ANPVHLVTVR GLGYKLEG
