REH1_YEAST
ID REH1_YEAST Reviewed; 432 AA.
AC Q06709; D6VZ22; Q7LIF0;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Cytoplasmic 60S subunit biogenesis factor REH1;
DE AltName: Full=REI1-homolog 1;
DE AltName: Full=pre-60S factor REH1;
GN Name=REH1; OrderedLocusNames=YLR387C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP DOMAIN.
RX PubMed=9171100; DOI=10.1093/nar/25.12.2464;
RA Boehm S., Frishman D., Mewes H.-W.;
RT "Variations of the C2H2 zinc finger motif in the yeast genome and
RT classification of yeast zinc finger proteins.";
RL Nucleic Acids Res. 25:2464-2469(1997).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION, ASSOCIATION WITH PRE-60S PARTICLES, AND INTERACTION WITH NMD3;
RP LSG1 AND TIF6.
RX PubMed=19433447; DOI=10.1128/mcb.01582-08;
RA Parnell K.M., Bass B.L.;
RT "Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S
RT subunit maturation.";
RL Mol. Cell. Biol. 29:4014-4023(2009).
CC -!- FUNCTION: Pre-60S-associated cytoplasmic factor involved in the
CC cytoplasmic maturation of the 60S subunit. May act redundantly with
CC REI1 to directly promote a stabilizing structural rearrangement in
CC cytoplasmic 60S subunit maturation independent on the REI1-specific
CC ARX1 recycling. {ECO:0000269|PubMed:19433447}.
CC -!- SUBUNIT: Associates with nascent pre-60S particles that have not yet
CC entered the translating pool, and is released from mature 60S subunits.
CC Interacts with pre-60S factors NMD3, LSG1, and TIF6.
CC {ECO:0000269|PubMed:19433447}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 2240 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the REI1 family. {ECO:0000305}.
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DR EMBL; U19104; AAB67280.1; -; Genomic_DNA.
DR EMBL; U19729; AAB82362.1; -; Genomic_DNA.
DR EMBL; AY692733; AAT92752.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09688.1; -; Genomic_DNA.
DR PIR; S51474; S51474.
DR RefSeq; NP_013491.1; NM_001182276.1.
DR PDB; 5H4P; EM; 3.07 A; z=377-432.
DR PDB; 6QIK; EM; 3.10 A; z=1-432.
DR PDB; 6QT0; EM; 3.40 A; z=1-432.
DR PDB; 6QTZ; EM; 3.50 A; z=1-432.
DR PDB; 6RI5; EM; 3.30 A; z=1-432.
DR PDB; 6S05; EM; 3.90 A; z=1-432.
DR PDBsum; 5H4P; -.
DR PDBsum; 6QIK; -.
DR PDBsum; 6QT0; -.
DR PDBsum; 6QTZ; -.
DR PDBsum; 6RI5; -.
DR PDBsum; 6S05; -.
DR AlphaFoldDB; Q06709; -.
DR SMR; Q06709; -.
DR BioGRID; 31646; 62.
DR DIP; DIP-4235N; -.
DR IntAct; Q06709; 2.
DR MINT; Q06709; -.
DR STRING; 4932.YLR387C; -.
DR iPTMnet; Q06709; -.
DR MaxQB; Q06709; -.
DR PaxDb; Q06709; -.
DR PRIDE; Q06709; -.
DR EnsemblFungi; YLR387C_mRNA; YLR387C; YLR387C.
DR GeneID; 851103; -.
DR KEGG; sce:YLR387C; -.
DR SGD; S000004379; REH1.
DR VEuPathDB; FungiDB:YLR387C; -.
DR eggNOG; KOG2785; Eukaryota.
DR GeneTree; ENSGT00390000018047; -.
DR HOGENOM; CLU_018787_1_1_1; -.
DR InParanoid; Q06709; -.
DR OMA; IPITHCF; -.
DR BioCyc; YEAST:G3O-32453-MON; -.
DR PRO; PR:Q06709; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q06709; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IGI:SGD.
DR InterPro; IPR041661; ZN622/Rei1/Reh1_Znf-C2H2.
DR InterPro; IPR040025; Znf622/Rei1/Reh1.
DR PANTHER; PTHR13182; PTHR13182; 1.
DR Pfam; PF12756; zf-C2H2_2; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Metal-binding; Reference proteome; Repeat;
KW Ribosome biogenesis; Zinc; Zinc-finger.
FT CHAIN 1..432
FT /note="Cytoplasmic 60S subunit biogenesis factor REH1"
FT /id="PRO_0000268700"
FT ZN_FING 6..30
FT /note="C2H2-type 1"
FT ZN_FING 186..209
FT /note="C2H2-type 2"
FT ZN_FING 237..261
FT /note="C2H2-type 3"
FT REGION 79..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..102
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 432 AA; 49690 MW; 1E4051797FD7D38A CRC64;
MSSTFFTCNC CVIQFKTSDL QRYHMKTEWH RYNLKRRIAN LPPIGAEQFA EKLQISEKEQ
AENQVDEFGF PVLKPVMNQS NALPQKQKKP IKSKRGRKVG TNLLKRKDRD IAKEKQNRSV
SPSGSISSQL SNLTVGTENT NTDYGEDTVS EYGFTSDSNY EYATSDEELD IADKPSDKEN
EKITITECIY CGKDNKEVER NVKHMFSEHG LFIPERSYLI DLNGLLEFLI KMIVIDHNCL
CCNFHGSGLE SIRAHMASKR HCRLPYETKE ERQLFAPFYD FTYDDHSISK NLQNDRAITS
KLSSVYGAKN DEEDGEVDIT LVSSENDINA NYTTVSIDES GLELTLPTGA RLGHRAGQRY
YRQNLPSQPN PNESRRTITA ADRRMVSGVT EKQYKKGMKK MQQLEKNAIN TQIRREIKRV
NFQTHYRDEL LQ
