ID   REI1_CAEEL              Reviewed;         244 AA.
AC   P42171;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Guanine nucleotide exchange factor rei-1 {ECO:0000305};
GN   Name=rei-1 {ECO:0000312|WormBase:C03C10.4};
GN   ORFNames=C03C10.4 {ECO:0000312|WormBase:C03C10.4};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, SUBUNIT, INTERACTION WITH RAB-11.1, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=26506309; DOI=10.1016/j.devcel.2015.09.013;
RA   Sakaguchi A., Sato M., Sato K., Gengyo-Ando K., Yorimitsu T., Nakai J.,
RA   Hara T., Sato K., Sato K.;
RT   "REI-1 is a guanine nucleotide exchange factor regulating RAB-11
RT   localization and function in C. elegans embryos.";
RL   Dev. Cell 35:211-221(2015).
CC   -!- FUNCTION: Guanine nucleotide exchange factor for Rab GTPase Rab-11.1.
CC       Spatially and temporally regulates the distribution of Rab-11.1 to
CC       target membranes during embryogenesis. Plays a role in cytokinesis,
CC       probably by targeting rab-11.1 to the cleavage furrows.
CC       {ECO:0000269|PubMed:26506309}.
CC   -!- SUBUNIT: Homodimer, tetramer and multimer. Interacts with rab-11.1.
CC       Binds preferentially to the GDP-bound form of rab-11.1.
CC       {ECO:0000269|PubMed:26506309}.
CC   -!- INTERACTION:
CC       P42171; Q22174: CELE_T04H1.2; NbExp=3; IntAct=EBI-314321, EBI-311938;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic granule
CC       {ECO:0000269|PubMed:26506309}. Golgi apparatus membrane
CC       {ECO:0000269|PubMed:26506309}. Note=Localizes to cytoplasmic granules
CC       and to Golgi apparatus membrane in growing oocytes. Co-localizes with
CC       rab-11.1 at Golgi apparatus membrane in embryos.
CC       {ECO:0000269|PubMed:26506309}.
CC   -!- TISSUE SPECIFICITY: Expressed in germ cells.
CC       {ECO:0000269|PubMed:26506309}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis.
CC       {ECO:0000269|PubMed:26506309}.
CC   -!- DISRUPTION PHENOTYPE: Viable. Disrupted localization of rab-11.1 and
CC       prolonged cytokinesis in embryos. Double knockout with rei-2 results in
CC       a smaller brood size, weak embryonic lethality, enhanced
CC       mislocalization of rab-11.1 and prolonged cytokinesis in embryos.
CC       {ECO:0000269|PubMed:26506309}.
CC   -!- SIMILARITY: Belongs to the SH3BP5 family. {ECO:0000305}.
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DR   EMBL; Z35637; CAA84689.1; -; Genomic_DNA.
DR   PIR; T18877; T18877.
DR   RefSeq; NP_497822.1; NM_065421.3.
DR   AlphaFoldDB; P42171; -.
DR   SMR; P42171; -.
DR   BioGRID; 40763; 13.
DR   DIP; DIP-26110N; -.
DR   IntAct; P42171; 12.
DR   STRING; 6239.C03C10.4; -.
DR   EPD; P42171; -.
DR   PaxDb; P42171; -.
DR   EnsemblMetazoa; C03C10.4.1; C03C10.4.1; WBGene00007270.
DR   GeneID; 175526; -.
DR   KEGG; cel:CELE_C03C10.4; -.
DR   UCSC; C03C10.4; c. elegans.
DR   CTD; 175526; -.
DR   WormBase; C03C10.4; CE00875; WBGene00007270; rei-1.
DR   eggNOG; KOG2008; Eukaryota.
DR   GeneTree; ENSGT00390000018500; -.
DR   HOGENOM; CLU_1107939_0_0_1; -.
DR   InParanoid; P42171; -.
DR   OMA; DINSYEM; -.
DR   OrthoDB; 1288889at2759; -.
DR   PhylomeDB; P42171; -.
DR   PRO; PR:P42171; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00007270; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:1990676; C:Golgi trans cisterna membrane; IDA:WormBase.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:WormBase.
DR   GO; GO:0004860; F:protein kinase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR   GO; GO:0061099; P:negative regulation of protein tyrosine kinase activity; IBA:GO_Central.
DR   GO; GO:1903292; P:protein localization to Golgi membrane; IMP:WormBase.
DR   InterPro; IPR007940; SH3BP5.
DR   PANTHER; PTHR19423; PTHR19423; 1.
DR   Pfam; PF05276; SH3BP5; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Coiled coil; Golgi apparatus; GTP-binding;
KW   Membrane; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..244
FT                   /note="Guanine nucleotide exchange factor rei-1"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000065128"
FT   REGION          221..244
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          6..39
FT                   /evidence="ECO:0000255"
FT   COILED          81..144
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   244 AA;  28323 MW;  6AA722860DE5DE7F CRC64;
     MTEGDDQLIS IRKQLENLNN ATDDINSYEM KLETVKKQFC ETQLMFNKEM LGIPKKLAKH
     ISKSRQFFDL KSRESEIRRC VQQAAAQFER QKTSVEMARE QVQILHNSLN NNQELDAEKQ
     YVDVIEQQLE LVKEAEGECL KAEKCHASRV RDLLQLEMAL RKCLEENGSA IKKSRPYYER
     KEVLTRTMNS QLELMSILEH EVQERKDSYS DSMRALEQIS DQIHQERSSQ SSLAPSSDAE
     SDSS