ID   REL1_RAT                Reviewed;         186 AA.
AC   P01347; Q78N50;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 144.
DE   RecName: Full=Prorelaxin 1;
DE   Contains:
DE     RecName: Full=Relaxin B chain;
DE   Contains:
DE     RecName: Full=Relaxin A chain;
DE   Flags: Precursor;
GN   Name=Rln1; Synonyms=Rln;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7231533; DOI=10.1038/291127a0;
RA   Hudson P., Haley J., Cronk M., Shine J., Niall H.;
RT   "Molecular cloning and characterization of cDNA sequences coding for rat
RT   relaxin.";
RL   Nature 291:127-131(1981).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14659888; DOI=10.1016/j.gene.2003.09.015;
RA   Soloff M.S., Gal S., Hoare S., Peters C.A., Hunzicker-Dunn M.,
RA   Anderson G.D., Wood T.G.;
RT   "Cloning, characterization, and expression of the rat relaxin gene.";
RL   Gene 323:149-155(2003).
RN   [3]
RP   PROTEIN SEQUENCE OF 23-57 AND 163-186, AND PYROGLUTAMATE FORMATION AT
RP   GLN-163.
RX   PubMed=7004862; DOI=10.1210/endo-108-2-726;
RA   John M.J., Borjesson B.W., Walsh J.R., Niall H.D.;
RT   "Limited sequence homology between porcine and rat relaxins: implications
RT   for physiological studies.";
RL   Endocrinology 108:726-729(1981).
CC   -!- FUNCTION: Relaxin is an ovarian hormone that acts with estrogen to
CC       produce dilatation of the birth canal in many mammals.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; J00780; AAA42029.1; -; mRNA.
DR   EMBL; V01264; CAA24578.1; -; mRNA.
DR   EMBL; AY240029; AAP41739.1; -; Genomic_DNA.
DR   PIR; A01614; RXRT.
DR   RefSeq; NP_038199.1; NM_013413.1.
DR   AlphaFoldDB; P01347; -.
DR   STRING; 10116.ENSRNOP00000021315; -.
DR   PaxDb; P01347; -.
DR   GeneID; 25616; -.
DR   KEGG; rno:25616; -.
DR   UCSC; RGD:3579; rat.
DR   CTD; 6013; -.
DR   RGD; 3579; Rln1.
DR   VEuPathDB; HostDB:ENSRNOG00000060867; -.
DR   eggNOG; ENOG502TH8D; Eukaryota.
DR   HOGENOM; CLU_115657_0_0_1; -.
DR   InParanoid; P01347; -.
DR   OMA; TINMMSE; -.
DR   OrthoDB; 1331287at2759; -.
DR   PhylomeDB; P01347; -.
DR   TreeFam; TF333404; -.
DR   Reactome; R-RNO-444821; Relaxin receptors.
DR   PRO; PR:P01347; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000060867; Expressed in frontal cortex and 3 other tissues.
DR   Genevisible; P01347; RN.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; TAS:RGD.
DR   GO; GO:0005102; F:signaling receptor binding; ISO:RGD.
DR   GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IGI:MGI.
DR   GO; GO:0048589; P:developmental growth; ISO:RGD.
DR   GO; GO:0060443; P:mammary gland morphogenesis; ISO:RGD.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IMP:CACAO.
DR   GO; GO:0060618; P:nipple development; ISO:RGD.
DR   GO; GO:0060736; P:prostate gland growth; ISO:RGD.
DR   GO; GO:0042981; P:regulation of apoptotic process; ISO:RGD.
DR   GO; GO:0050878; P:regulation of body fluid levels; TAS:RGD.
DR   GO; GO:0042127; P:regulation of cell population proliferation; IMP:CACAO.
DR   GO; GO:0010749; P:regulation of nitric oxide mediated signal transduction; IDA:MGI.
DR   GO; GO:0007283; P:spermatogenesis; ISO:RGD.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022421; Relaxin.
DR   Pfam; PF00049; Insulin; 1.
DR   PRINTS; PR02004; RELAXIN.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Hormone; Pyrrolidone carboxylic acid; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:7004862"
FT   PEPTIDE         23..57
FT                   /note="Relaxin B chain"
FT                   /id="PRO_0000016116"
FT   PROPEP          58..158
FT                   /note="Connecting peptide"
FT                   /id="PRO_0000016117"
FT   PEPTIDE         163..186
FT                   /note="Relaxin A chain"
FT                   /id="PRO_0000016118"
FT   MOD_RES         163
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:7004862"
FT   DISULFID        36..173
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        48..186
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        172..177
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   186 AA;  20489 MW;  08BAEC79BCF0E80F CRC64;
     MSSRLLLQLL GFWLFLSQPC RARVSEEWMD QVIQVCGRGY ARAWIEVCGA SVGRLALSQE
     EPAPLARQAT AEVVPSFINK DAEPFDMTLK CLPNLSEERK AALSEGRAPF PELQQHAPAL
     SDSVVSLEGF KKTFHNQLGE AEDGGPPELK YLGSDAQSRK KRQSGALLSE QCCHIGCTRR
     SIAKLC