ID   REL3_MOUSE              Reviewed;         141 AA.
AC   Q8CHK2;
DT   07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Relaxin-3;
DE   AltName: Full=Insulin-like peptide INSL7;
DE            Short=Insulin-like peptide 7;
DE   AltName: Full=Prorelaxin M3;
DE   Contains:
DE     RecName: Full=Relaxin-3 B chain;
DE   Contains:
DE     RecName: Full=Relaxin-3 A chain;
DE   Flags: Precursor;
GN   Name=Rln3; Synonyms=Insl7;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=12686464; DOI=10.1016/s0167-0115(02)00304-x;
RA   Kizawa H., Nishi K., Ishibashi Y., Harada M., Asano T., Ito Y., Suzuki N.,
RA   Hinuma S., Fujisawa Y., Onda H., Nishimura O., Fujino M.;
RT   "Production of recombinant human relaxin 3 in AtT20 cells.";
RL   Regul. Pept. 113:79-84(2003).
RN   [2]
RP   TISSUE SPECIFICITY.
RX   PubMed=11689565; DOI=10.1074/jbc.m107882200;
RA   Bathgate R.A.D., Samuel C.S., Burazin T.C.D., Layfield S., Claasz A.A.,
RA   Reytomas I.G.T., Dawson N.F., Zhao C., Bond C., Summers R.J., Parry L.J.,
RA   Wade J.D., Tregear G.W.;
RT   "Human relaxin gene 3 (H3) and the equivalent mouse relaxin (M3) gene.
RT   Novel members of the relaxin peptide family.";
RL   J. Biol. Chem. 277:1148-1157(2002).
CC   -!- FUNCTION: May play a role in neuropeptide signaling processes. Ligand
CC       for LGR7, relaxin-3 receptor-1 and relaxin-3 receptor-2 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: High expression in the brain localized to the
CC       pons/medulla with highest levels in pars ventromedialis of the dorsal
CC       tegmental nucleus. Significant expression is also detected in the
CC       spleen, thymus, lung, testis and ovary. {ECO:0000269|PubMed:11689565}.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; AB076565; BAC53760.1; -; mRNA.
DR   CCDS; CCDS22466.1; -.
DR   RefSeq; NP_775276.1; NM_173184.1.
DR   AlphaFoldDB; Q8CHK2; -.
DR   STRING; 10090.ENSMUSP00000051392; -.
DR   PhosphoSitePlus; Q8CHK2; -.
DR   PaxDb; Q8CHK2; -.
DR   PRIDE; Q8CHK2; -.
DR   ProteomicsDB; 255179; -.
DR   Antibodypedia; 50312; 149 antibodies from 23 providers.
DR   DNASU; 212108; -.
DR   Ensembl; ENSMUST00000061923; ENSMUSP00000051392; ENSMUSG00000045232.
DR   GeneID; 212108; -.
DR   KEGG; mmu:212108; -.
DR   UCSC; uc009mls.1; mouse.
DR   CTD; 117579; -.
DR   MGI; MGI:2158015; Rln3.
DR   VEuPathDB; HostDB:ENSMUSG00000045232; -.
DR   eggNOG; ENOG502S2C4; Eukaryota.
DR   GeneTree; ENSGT00940000154396; -.
DR   HOGENOM; CLU_120043_0_0_1; -.
DR   InParanoid; Q8CHK2; -.
DR   OMA; CEWGCSK; -.
DR   OrthoDB; 1354498at2759; -.
DR   PhylomeDB; Q8CHK2; -.
DR   TreeFam; TF333404; -.
DR   Reactome; R-MMU-418555; G alpha (s) signalling events.
DR   Reactome; R-MMU-418594; G alpha (i) signalling events.
DR   Reactome; R-MMU-444821; Relaxin receptors.
DR   BioGRID-ORCS; 212108; 2 hits in 72 CRISPR screens.
DR   ChiTaRS; Rln3; mouse.
DR   PRO; PR:Q8CHK2; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q8CHK2; protein.
DR   Bgee; ENSMUSG00000045232; Expressed in secondary oocyte and 24 other tissues.
DR   GO; GO:0005576; C:extracellular region; TAS:MGI.
DR   GO; GO:0001664; F:G protein-coupled receptor binding; ISO:MGI.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022352; Insulin_family.
DR   Pfam; PF00049; Insulin; 1.
DR   PRINTS; PR00276; INSULINFAMLY.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   2: Evidence at transcript level;
KW   Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000250"
FT   PEPTIDE         25..51
FT                   /note="Relaxin-3 B chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000016085"
FT   PROPEP          54..117
FT                   /note="Connecting peptide"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000016086"
FT   PEPTIDE         118..141
FT                   /note="Relaxin-3 A chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000016087"
FT   DISULFID        34..128
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        46..141
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        127..132
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   141 AA;  14926 MW;  0899CD9F57552951 CRC64;
     MAMLGLLLLA SWALLGALGL QAEARPAPYG VKLCGREFIR AVIFTCGGSR WRRADILAHE
     SLGDFFADGE ANTDHLASEL DEAVGSSEWL ALTKSPQAFY GGRASWQGSP GVVRGSRDVL
     AGLSSSCCEW GCSKSQISSL C