REL3_PIG
ID REL3_PIG Reviewed; 140 AA.
AC Q8HY17; A7YKU2;
DT 07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Relaxin-3;
DE AltName: Full=Insulin-like peptide INSL7;
DE Short=Insulin-like peptide 7;
DE Contains:
DE RecName: Full=Relaxin-3 B chain;
DE Contains:
DE RecName: Full=Relaxin-3 A chain;
DE Flags: Precursor;
GN Name=RLN3; Synonyms=INSL7;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12686464; DOI=10.1016/s0167-0115(02)00304-x;
RA Kizawa H., Nishi K., Ishibashi Y., Harada M., Asano T., Ito Y., Suzuki N.,
RA Hinuma S., Fujisawa Y., Onda H., Nishimura O., Fujino M.;
RT "Production of recombinant human relaxin 3 in AtT20 cells.";
RL Regul. Pept. 113:79-84(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Chomdej S., Yammuen-Art S., Jonas E., Jennen D., Ponsuksili S.,
RA Schellander K., Wimmers K.;
RT "Comparing the expression of two different relaxin genes in pig and the
RT association of RLN1 and RLN3 to teat development in pigs.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP INTERACTION WITH LGR7.
RX PubMed=12506116; DOI=10.1074/jbc.m212457200;
RA Sudo S., Kumagai J., Nishi S., Layfield S., Ferraro T., Bathgate R.A.D.,
RA Hsueh A.J.W.;
RT "H3 relaxin is a specific ligand for LGR7 and activates the receptor by
RT interacting with both the ectodomain and the exoloop 2.";
RL J. Biol. Chem. 278:7855-7862(2003).
RN [4]
RP PROTEIN SEQUENCE OF 27-53 AND 117-140, AND INTERACTION WITH RELAXIN-3
RP RECEPTOR-1.
RX PubMed=14522968; DOI=10.1074/jbc.m308995200;
RA Liu C., Eriste E., Sutton S., Chen J., Roland B., Kuei C., Farmer N.,
RA Joernvall H., Sillard R., Lovenberg T.W.;
RT "Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan
RT G-protein coupled receptor GPCR135.";
RL J. Biol. Chem. 278:50754-50764(2003).
CC -!- FUNCTION: May play a role in neuropeptide signaling processes. Ligand
CC for LGR7, RXFP3 and RXFP4 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB076661; BAC53769.1; -; Genomic_DNA.
DR EMBL; DQ974115; ABJ98405.1; -; Genomic_DNA.
DR RefSeq; NP_001039144.1; NM_001045679.1.
DR RefSeq; XP_013850120.1; XM_013994666.1.
DR RefSeq; XP_013850121.1; XM_013994667.1.
DR AlphaFoldDB; Q8HY17; -.
DR SMR; Q8HY17; -.
DR STRING; 9823.ENSSSCP00000014634; -.
DR PaxDb; Q8HY17; -.
DR PRIDE; Q8HY17; -.
DR Ensembl; ENSSSCT00000015039; ENSSSCP00000014634; ENSSSCG00000013765.
DR Ensembl; ENSSSCT00035070298; ENSSSCP00035028515; ENSSSCG00035052734.
DR Ensembl; ENSSSCT00040105672; ENSSSCP00040048442; ENSSSCG00040075986.
DR Ensembl; ENSSSCT00055003339; ENSSSCP00055002505; ENSSSCG00055001823.
DR Ensembl; ENSSSCT00070001253; ENSSSCP00070001097; ENSSSCG00070000670.
DR GeneID; 503836; -.
DR KEGG; ssc:503836; -.
DR CTD; 117579; -.
DR VGNC; VGNC:92329; RLN3.
DR eggNOG; ENOG502S2C4; Eukaryota.
DR GeneTree; ENSGT00940000154396; -.
DR HOGENOM; CLU_120043_0_0_1; -.
DR InParanoid; Q8HY17; -.
DR OMA; CEWGCSK; -.
DR OrthoDB; 1354498at2759; -.
DR TreeFam; TF333404; -.
DR Reactome; R-SSC-418555; G alpha (s) signalling events.
DR Reactome; R-SSC-418594; G alpha (i) signalling events.
DR Reactome; R-SSC-444821; Relaxin receptors.
DR Proteomes; UP000008227; Chromosome 2.
DR Proteomes; UP000314985; Chromosome 2.
DR Bgee; ENSSSCG00000013765; Expressed in testis and 13 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR InterPro; IPR022352; Insulin_family.
DR Pfam; PF00049; Insulin; 1.
DR PRINTS; PR00276; INSULINFAMLY.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; SSF56994; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000269|PubMed:14522968"
FT PEPTIDE 27..53
FT /note="Relaxin-3 B chain"
FT /id="PRO_0000016091"
FT PROPEP 56..116
FT /note="Connecting peptide"
FT /evidence="ECO:0000269|PubMed:14522968"
FT /id="PRO_0000016092"
FT PEPTIDE 117..140
FT /note="Relaxin-3 A chain"
FT /id="PRO_0000016093"
FT DISULFID 36..127
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 48..140
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 126..131
FT /evidence="ECO:0000250"
SQ SEQUENCE 140 AA; 15360 MW; 063775801F6803B9 CRC64;
MAKRPLLLLL LAVWVLAGEL WLRTEARASP YGVKLCGREF IRAVIFTCGG SRWRRSDMLA
HEALGDVFSD TDSNADSELD EAMASSEWLA LTKSPETFYG VQPGWQRTPG ALRGSRDVLA
GLSSNCCKWG CSKSEISSLC
