ID   RELE3_METJA             Reviewed;          88 AA.
AC   Q58503;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Toxin RelE3;
DE            EC=3.1.-.-;
DE   AltName: Full=MjRelE;
DE   AltName: Full=Putative endoribonuclease RelE;
GN   Name=relE3; Synonyms=relE; OrderedLocusNames=MJ1103;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH RELB, SUBUNIT, AND
RP   MUTAGENESIS OF ARG-62.
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=19712680; DOI=10.1016/j.jmb.2009.08.048;
RA   Francuski D., Saenger W.;
RT   "Crystal structure of the antitoxin-toxin protein complex RelB-RelE from
RT   Methanococcus jannaschii.";
RL   J. Mol. Biol. 393:898-908(2009).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. Has
CC       RNase activity (By similarity). Is very toxic upon expression in
CC       E.coli. Its toxic activity is probably neutralized by the cognate
CC       antitoxin RelB3. {ECO:0000250}.
CC   -!- SUBUNIT: Forms heterodimers with RelB3 and possibly a heterotetramer
CC       RelE3-RelB3(2)-RelE3 from 2 heterodimers. The heterotetramer is
CC       probably not very stable in solution. {ECO:0000269|PubMed:19712680}.
CC   -!- SIMILARITY: Belongs to the RelE toxin family. {ECO:0000305}.
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DR   EMBL; L77117; AAB99106.1; -; Genomic_DNA.
DR   PIR; F64437; F64437.
DR   RefSeq; WP_010870615.1; NC_000909.1.
DR   PDB; 3BPQ; X-ray; 2.20 A; B/D=1-88.
DR   PDBsum; 3BPQ; -.
DR   AlphaFoldDB; Q58503; -.
DR   SMR; Q58503; -.
DR   STRING; 243232.MJ_1103; -.
DR   EnsemblBacteria; AAB99106; AAB99106; MJ_1103.
DR   GeneID; 1452000; -.
DR   KEGG; mja:MJ_1103; -.
DR   eggNOG; arCOG01665; Archaea.
DR   HOGENOM; CLU_155761_5_1_2; -.
DR   OMA; MKVLFAK; -.
DR   OrthoDB; 125851at2157; -.
DR   EvolutionaryTrace; Q58503; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2310.20; -; 1.
DR   InterPro; IPR007712; RelE/ParE_toxin.
DR   InterPro; IPR035093; RelE/ParE_toxin_dom_sf.
DR   Pfam; PF05016; ParE_toxin; 1.
DR   SUPFAM; SSF143011; SSF143011; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..88
FT                   /note="Toxin RelE3"
FT                   /id="PRO_0000107168"
FT   MUTAGEN         62
FT                   /note="R->K,S: Inactivates toxin."
FT                   /evidence="ECO:0000269|PubMed:19712680"
FT   STRAND          4..6
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   HELIX           7..13
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   HELIX           18..34
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   STRAND          44..46
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   STRAND          50..58
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   STRAND          61..68
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   STRAND          71..79
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   HELIX           80..83
FT                   /evidence="ECO:0007829|PDB:3BPQ"
FT   HELIX           84..86
FT                   /evidence="ECO:0007829|PDB:3BPQ"
SQ   SEQUENCE   88 AA;  10562 MW;  DD58E41BCBE59F5D CRC64;
     MKVLFAKTFV KDLKHVPGHI RKRIKLIIEE CQNSNSLNDL KLDIKKIKGY HNYYRIRVGN
     YRIGIEVNGD TIIFRRVLHR KSIYDYFP