ID   RELK_MYCTO              Reviewed;          85 AA.
AC   P9WF08; L0TCJ4; O50387; P64528;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Toxin RelK;
DE            EC=3.1.-.-;
DE   AltName: Full=Endoribonuclease YoeB;
DE   AltName: Full=Putative mRNA interferase RelE3;
DE   AltName: Full=Putative mRNA interferase YoeB;
DE   AltName: Full=Toxin YoeB;
GN   Name=relK; Synonyms=relE3, yoeB; OrderedLocusNames=MT3466;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. Has
CC       RNase activity and preferentially cleaves at the 3'-end of purine
CC       ribonucleotides (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a toxin-antitoxin complex with RelJ. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the YoeB family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK47805.1; -; Genomic_DNA.
DR   PIR; E70970; E70970.
DR   RefSeq; WP_003417760.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF08; -.
DR   SMR; P9WF08; -.
DR   EnsemblBacteria; AAK47805; AAK47805; MT3466.
DR   GeneID; 45427357; -.
DR   KEGG; mtc:MT3466; -.
DR   PATRIC; fig|83331.31.peg.3725; -.
DR   HOGENOM; CLU_169492_2_2_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:InterPro.
DR   Gene3D; 3.30.2310.20; -; 1.
DR   InterPro; IPR035093; RelE/ParE_toxin_dom_sf.
DR   InterPro; IPR009614; YoeB_toxin.
DR   PANTHER; PTHR38039; PTHR38039; 1.
DR   Pfam; PF06769; YoeB_toxin; 1.
DR   SUPFAM; SSF143011; SSF143011; 1.
DR   TIGRFAMs; TIGR02116; toxin_Txe_YoeB; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Endonuclease; Hydrolase; Nuclease; Repressor;
KW   Toxin-antitoxin system; Transcription; Transcription regulation.
FT   CHAIN           1..85
FT                   /note="Toxin RelK"
FT                   /id="PRO_0000428621"
FT   ACT_SITE        47
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        84
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   85 AA;  10102 MW;  8663A289B5FD9DAF CRC64;
     MRSVNFDPDA WEDFLFWLAA DRKTARRITR LIGEIQRDPF SGIGKPEPLQ GELSGYWSRR
     IDDEHRLVYR AGDDEVTMLK ARYHY