ID   RELX_HORSE              Reviewed;         182 AA.
AC   P22969; Q28907;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Prorelaxin;
DE            Short=RXN;
DE   Contains:
DE     RecName: Full=Relaxin B chain;
DE   Contains:
DE     RecName: Full=Relaxin A chain;
DE   Flags: Precursor;
GN   Name=RLN;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Hokkaido; TISSUE=Placenta;
RA   Min K., Shiota K., Ogawa T.;
RT   "Molecular cloning of equine preprorelaxin cDNA.";
RL   J. Reprod. Dev. 42:171-178(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 32-174.
RC   TISSUE=Placenta;
RX   PubMed=7543295; DOI=10.1095/biolreprod52.6.1307;
RA   Klonisch T., Ryan P.L., Yamashiro S., Porter D.G.;
RT   "Partial complementary deoxyribonucleic acid cloning of equine relaxin
RT   messenger ribonucleic acid, and its localization within the equine
RT   placenta.";
RL   Biol. Reprod. 52:1307-1315(1995).
RN   [3]
RP   PROTEIN SEQUENCE OF 26-53 AND 163-182, PYROGLUTAMATE FORMATION AT GLN-26
RP   AND GLN-163, AND MASS SPECTROMETRY.
RC   TISSUE=Placenta;
RX   PubMed=2055195; DOI=10.1210/endo-129-1-375;
RA   Stewart D.R., Nevins B., Hadas E., Vandlen R.;
RT   "Affinity purification and sequence determination of equine relaxin.";
RL   Endocrinology 129:375-383(1991).
CC   -!- FUNCTION: Relaxin is an ovarian hormone that acts with estrogen to
CC       produce dilatation of the birth canal in many mammals.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: [Relaxin B chain]: Mass=2855.2; Method=FAB;
CC       Note=With pyrrolidone carboxylic acid at Gln-26, isoform lacking 52-WK-
CC       53.; Evidence={ECO:0000269|PubMed:2055195};
CC   -!- MASS SPECTROMETRY: [Relaxin A chain]: Mass=2397.4; Method=FAB;
CC       Note=With pyrrolidone carboxylic acid at Gln-163.;
CC       Evidence={ECO:0000269|PubMed:2055195};
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; AB000201; BAA19069.1; -; mRNA.
DR   EMBL; S78800; AAB35036.1; -; mRNA.
DR   PIR; B49739; A49739.
DR   PIR; I47053; I47053.
DR   RefSeq; NP_001075278.1; NM_001081809.1.
DR   AlphaFoldDB; P22969; -.
DR   PaxDb; P22969; -.
DR   PRIDE; P22969; -.
DR   GeneID; 100033823; -.
DR   KEGG; ecb:100033823; -.
DR   CTD; 100329416; -.
DR   InParanoid; P22969; -.
DR   OrthoDB; 1331287at2759; -.
DR   Proteomes; UP000002281; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022421; Relaxin.
DR   Pfam; PF00049; Insulin; 1.
DR   PRINTS; PR02004; RELAXIN.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Hormone; Pyrrolidone carboxylic acid; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:2055195"
FT   PEPTIDE         26..53
FT                   /note="Relaxin B chain"
FT                   /id="PRO_0000016073"
FT   PROPEP          54..156
FT                   /note="Connecting peptide"
FT                   /id="PRO_0000016074"
FT   PROPEP          161..162
FT                   /evidence="ECO:0000269|PubMed:2055195"
FT                   /id="PRO_0000278776"
FT   PEPTIDE         163..182
FT                   /note="Relaxin A chain"
FT                   /id="PRO_0000016075"
FT   MOD_RES         26
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:2055195"
FT   MOD_RES         163
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:2055195"
FT   DISULFID        35..169
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        47..182
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        168..173
FT                   /evidence="ECO:0000250"
FT   CONFLICT        66
FT                   /note="A -> V (in Ref. 2; AAB35036)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        133
FT                   /note="L -> Q (in Ref. 2; AAB35036)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   182 AA;  20721 MW;  E5C9414303A838B8 CRC64;
     MRRLFLSHVL GAWLLLSQLP RELSGQKPDD VIKACGRELA RLRIEICGSL SWKKTVLRLE
     EPGLEAGQPV EIVSSSISKD AEALNTKLGL NSNLPKEQKA TLSERQPSWR ELLQQPALKD
     SNLNLEEFEE TILKTQSEVE DDSLSELKNL GLDKHSRKKR MIQLSHKCCY WGCTRKELAR
     QC