REM2_HUMAN
ID REM2_HUMAN Reviewed; 340 AA.
AC Q8IYK8; B7Z5P1; Q8N8R8;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 02-MAR-2010, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=GTP-binding protein REM 2;
DE AltName: Full=Rad and Gem-like GTP-binding protein 2;
GN Name=REM2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS) OF 110-286 IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "Crystal structure of the human rem2 GTPase with bound GDP.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Binds GTP saturably and exhibits a low intrinsic rate of GTP
CC hydrolysis. {ECO:0000250|UniProtKB:Q9WTY2}.
CC -!- INTERACTION:
CC Q8IYK8; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-11304833, EBI-3867333;
CC Q8IYK8; Q07627: KRTAP1-1; NbExp=3; IntAct=EBI-11304833, EBI-11959885;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9WTY2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IYK8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IYK8-2; Sequence=VSP_056922, VSP_056923;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. RGK family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH35663.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC04746.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK096283; BAC04746.1; ALT_INIT; mRNA.
DR EMBL; AK299238; BAH12977.1; -; mRNA.
DR EMBL; AL135998; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC035663; AAH35663.1; ALT_INIT; mRNA.
DR CCDS; CCDS45082.1; -. [Q8IYK8-1]
DR RefSeq; NP_775798.2; NM_173527.2. [Q8IYK8-1]
DR PDB; 3CBQ; X-ray; 1.82 A; A=110-286.
DR PDBsum; 3CBQ; -.
DR AlphaFoldDB; Q8IYK8; -.
DR SMR; Q8IYK8; -.
DR BioGRID; 127778; 3.
DR IntAct; Q8IYK8; 3.
DR STRING; 9606.ENSP00000267396; -.
DR iPTMnet; Q8IYK8; -.
DR PhosphoSitePlus; Q8IYK8; -.
DR BioMuta; REM2; -.
DR DMDM; 290457655; -.
DR EPD; Q8IYK8; -.
DR jPOST; Q8IYK8; -.
DR MassIVE; Q8IYK8; -.
DR PaxDb; Q8IYK8; -.
DR PeptideAtlas; Q8IYK8; -.
DR PRIDE; Q8IYK8; -.
DR ProteomicsDB; 6706; -.
DR ProteomicsDB; 71193; -. [Q8IYK8-1]
DR Antibodypedia; 22314; 52 antibodies from 21 providers.
DR DNASU; 161253; -.
DR Ensembl; ENST00000267396.9; ENSP00000267396.4; ENSG00000139890.10. [Q8IYK8-1]
DR Ensembl; ENST00000536884.1; ENSP00000442774.1; ENSG00000139890.10. [Q8IYK8-2]
DR GeneID; 161253; -.
DR KEGG; hsa:161253; -.
DR MANE-Select; ENST00000267396.9; ENSP00000267396.4; NM_173527.3; NP_775798.2.
DR UCSC; uc001whf.2; human. [Q8IYK8-1]
DR CTD; 161253; -.
DR DisGeNET; 161253; -.
DR GeneCards; REM2; -.
DR HGNC; HGNC:20248; REM2.
DR HPA; ENSG00000139890; Tissue enhanced (bone).
DR neXtProt; NX_Q8IYK8; -.
DR OpenTargets; ENSG00000139890; -.
DR PharmGKB; PA134916581; -.
DR VEuPathDB; HostDB:ENSG00000139890; -.
DR eggNOG; KOG0395; Eukaryota.
DR GeneTree; ENSGT00940000160062; -.
DR HOGENOM; CLU_041217_3_1_1; -.
DR InParanoid; Q8IYK8; -.
DR OMA; DIWEQMQ; -.
DR OrthoDB; 679855at2759; -.
DR PhylomeDB; Q8IYK8; -.
DR TreeFam; TF314379; -.
DR BRENDA; 3.4.23.15; 2681.
DR PathwayCommons; Q8IYK8; -.
DR SignaLink; Q8IYK8; -.
DR BioGRID-ORCS; 161253; 11 hits in 1069 CRISPR screens.
DR EvolutionaryTrace; Q8IYK8; -.
DR GenomeRNAi; 161253; -.
DR Pharos; Q8IYK8; Tbio.
DR PRO; PR:Q8IYK8; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q8IYK8; protein.
DR Bgee; ENSG00000139890; Expressed in blood and 92 other tissues.
DR Genevisible; Q8IYK8; HS.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005246; F:calcium channel regulator activity; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IEA:Ensembl.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51421; RAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; GTP-binding; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..340
FT /note="GTP-binding protein REM 2"
FT /id="PRO_0000122483"
FT REGION 1..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 283..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 121..128
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|Ref.4"
FT BINDING 229..232
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|Ref.4"
FT BINDING 260..261
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|Ref.4"
FT MOD_RES 27
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9WTY2"
FT MOD_RES 295
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9WTY2"
FT VAR_SEQ 149..221
FT /note="EDTYERRIMVDKEEVTLVVYDIWEQGDAGGWLRDHCLQTGDAFLIVFSVTDR
FT RSFSKVPETLLRLRAGRPHHD -> GGCRRVAAGPLPSDRGRLSHRLLSHRPTEFLQSS
FT RDPTSAPGWEAAPRPTRYPRWKQERLGPLPGGITGGCVS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056922"
FT VAR_SEQ 222..340
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056923"
FT VARIANT 96
FT /note="G -> A (in dbSNP:rs8014119)"
FT /id="VAR_055938"
FT STRAND 114..120
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 127..133
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 141..143
FT /evidence="ECO:0007829|PDB:3CBQ"
FT TURN 144..146
FT /evidence="ECO:0007829|PDB:3CBQ"
FT STRAND 151..158
FT /evidence="ECO:0007829|PDB:3CBQ"
FT STRAND 161..168
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 175..177
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 178..187
FT /evidence="ECO:0007829|PDB:3CBQ"
FT STRAND 189..196
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 200..204
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 206..216
FT /evidence="ECO:0007829|PDB:3CBQ"
FT STRAND 224..229
FT /evidence="ECO:0007829|PDB:3CBQ"
FT TURN 234..236
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 241..250
FT /evidence="ECO:0007829|PDB:3CBQ"
FT STRAND 254..259
FT /evidence="ECO:0007829|PDB:3CBQ"
FT TURN 260..263
FT /evidence="ECO:0007829|PDB:3CBQ"
FT HELIX 266..278
FT /evidence="ECO:0007829|PDB:3CBQ"
SQ SEQUENCE 340 AA; 37139 MW; 7043E32471EA18DA CRC64;
MHTDLDTDMD MDTETTALCP SGSRRASPPG TPTPEADATL LKKSEKLLAE LDRSGLPSAP
GAPRRRGSMP VPYKHQLRRA QAVDELDWPP QASSSGSSDS LGSGEAAPAQ KDGIFKVMLV
GESGVGKSTL AGTFGGLQGD SAHEPENPED TYERRIMVDK EEVTLVVYDI WEQGDAGGWL
RDHCLQTGDA FLIVFSVTDR RSFSKVPETL LRLRAGRPHH DLPVILVGNK SDLARSREVS
LEEGRHLAGT LSCKHIETSA ALHHNTRELF EGAVRQIRLR RGRNHAGGQR PDPGSPEGPA
PPARRESLTK KAKRFLANLV PRNAKFFKQR SRSCHDLSVL
