REM2_MOUSE
ID REM2_MOUSE Reviewed; 341 AA.
AC Q8VEL9; Q8BPB1;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 02-MAR-2010, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=GTP-binding protein REM 2;
DE AltName: Full=Rad and Gem-like GTP-binding protein 2;
GN Name=Rem2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Pituitary, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=129; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Binds GTP saturably and exhibits a low intrinsic rate of GTP
CC hydrolysis. {ECO:0000250|UniProtKB:Q9WTY2}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9WTY2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VEL9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VEL9-2; Sequence=VSP_038749;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. RGK family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH18219.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC36746.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK077317; BAC36746.1; ALT_INIT; mRNA.
DR EMBL; AK090165; BAC41120.1; -; mRNA.
DR EMBL; BC018219; AAH18219.1; ALT_INIT; mRNA.
DR CCDS; CCDS27090.2; -. [Q8VEL9-1]
DR RefSeq; NP_542764.2; NM_080726.3.
DR PDB; 3Q85; X-ray; 1.76 A; A/B=114-282.
DR PDBsum; 3Q85; -.
DR AlphaFoldDB; Q8VEL9; -.
DR SMR; Q8VEL9; -.
DR IntAct; Q8VEL9; 1.
DR MINT; Q8VEL9; -.
DR STRING; 10090.ENSMUSP00000127199; -.
DR iPTMnet; Q8VEL9; -.
DR PhosphoSitePlus; Q8VEL9; -.
DR MaxQB; Q8VEL9; -.
DR PaxDb; Q8VEL9; -.
DR PRIDE; Q8VEL9; -.
DR ProteomicsDB; 253218; -. [Q8VEL9-1]
DR ProteomicsDB; 253219; -. [Q8VEL9-2]
DR ABCD; Q8VEL9; 1 sequenced antibody.
DR DNASU; 140743; -.
DR GeneID; 140743; -.
DR KEGG; mmu:140743; -.
DR CTD; 161253; -.
DR MGI; MGI:2155260; Rem2.
DR eggNOG; KOG0395; Eukaryota.
DR InParanoid; Q8VEL9; -.
DR OrthoDB; 679855at2759; -.
DR PhylomeDB; Q8VEL9; -.
DR BioGRID-ORCS; 140743; 0 hits in 72 CRISPR screens.
DR EvolutionaryTrace; Q8VEL9; -.
DR PRO; PR:Q8VEL9; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8VEL9; protein.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005246; F:calcium channel regulator activity; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; ISO:MGI.
DR GO; GO:0003924; F:GTPase activity; ISO:MGI.
DR DisProt; DP02573; -.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51421; RAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; GTP-binding; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..341
FT /note="GTP-binding protein REM 2"
FT /id="PRO_0000122484"
FT REGION 1..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 84..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 282..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 122..129
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IYK8"
FT BINDING 230..233
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IYK8"
FT BINDING 261..262
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IYK8"
FT MOD_RES 27
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9WTY2"
FT MOD_RES 296
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9WTY2"
FT VAR_SEQ 1..69
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_038749"
FT CONFLICT 13..15
FT /note="TET -> IEI (in Ref. 2; AAH18219)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="G -> S (in Ref. 2; AAH18219)"
FT /evidence="ECO:0000305"
FT STRAND 115..121
FT /evidence="ECO:0007829|PDB:3Q85"
FT HELIX 128..136
FT /evidence="ECO:0007829|PDB:3Q85"
FT STRAND 152..159
FT /evidence="ECO:0007829|PDB:3Q85"
FT STRAND 162..169
FT /evidence="ECO:0007829|PDB:3Q85"
FT HELIX 184..188
FT /evidence="ECO:0007829|PDB:3Q85"
FT STRAND 190..197
FT /evidence="ECO:0007829|PDB:3Q85"
FT HELIX 201..205
FT /evidence="ECO:0007829|PDB:3Q85"
FT HELIX 207..217
FT /evidence="ECO:0007829|PDB:3Q85"
FT STRAND 225..230
FT /evidence="ECO:0007829|PDB:3Q85"
FT HELIX 235..237
FT /evidence="ECO:0007829|PDB:3Q85"
FT HELIX 242..251
FT /evidence="ECO:0007829|PDB:3Q85"
FT STRAND 255..258
FT /evidence="ECO:0007829|PDB:3Q85"
FT TURN 261..264
FT /evidence="ECO:0007829|PDB:3Q85"
FT HELIX 267..280
FT /evidence="ECO:0007829|PDB:3Q85"
SQ SEQUENCE 341 AA; 37367 MW; D7F415A9ABC86125 CRC64;
MHTDLDTDMD MDTETVALCS SSSRQASPLG TPTPEADTTL LKQKPEKLLA ELDLSGPPPA
PGVPRRRGSM PVPYKHQLRR AQAVDELDWP PQASPSGSSD SLGSGEAALT QKDGVFKVML
VGESGVGKST LAGTFGGLQG DHAHEMENSE DTYERRIMVD KEEVTLIVYD IWEQGDAGGW
LQDHCLQTGD AFLIVFSVTD RRGFSKVPET LLRLRAGRPH HDLPVILVGN KSDLARSREV
SLEEGRHLAG TLSCKHIETS AALHHNTREL FEGAVRQIRL RRGRGHAGGQ RPEPSSPDGP
APPTRRESLT KKAKRFLANL VPRNAKFFKQ RSRSCHDLSV L
