REMA_BACSU
ID REMA_BACSU Reviewed; 89 AA.
AC Q7WY72;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Extracellular matrix regulatory protein A {ECO:0000305};
DE AltName: Full=Regulator of extracellular matrix A {ECO:0000303|PubMed:19363116};
GN Name=remA {ECO:0000303|PubMed:19363116}; Synonyms=ylzA;
GN OrderedLocusNames=BSU15670;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19363116; DOI=10.1128/jb.00278-09;
RA Winkelman J.T., Blair K.M., Kearns D.B.;
RT "RemA (YlzA) and RemB (YaaB) regulate extracellular matrix operon
RT expression and biofilm formation in Bacillus subtilis.";
RL J. Bacteriol. 191:3981-3991(2009).
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=23396918; DOI=10.1128/jb.02110-12;
RA Abecasis A.B., Serrano M., Alves R., Quintais L., Pereira-Leal J.B.,
RA Henriques A.O.;
RT "A genomic signature and the identification of new sporulation genes.";
RL J. Bacteriol. 195:2101-2115(2013).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=23646920; DOI=10.1111/mmi.12235;
RA Winkelman J.T., Bree A.C., Bate A.R., Eichenberger P., Gourse R.L.,
RA Kearns D.B.;
RT "RemA is a DNA-binding protein that activates biofilm matrix gene
RT expression in Bacillus subtilis.";
RL Mol. Microbiol. 88:984-997(2013).
CC -!- FUNCTION: Regulates the biosynthesis of the extracellular matrix and
CC the biofilm formation (PubMed:19363116, PubMed:23646920). Activates the
CC transcription of the matrix biosynthesis operons eps and tapA-sipW-
CC tasA. Also activates expression of the opuA operon, involved in
CC osmoprotection. Acts by binding specifically to the promoter region of
CC the target genes (PubMed:23646920). Acts in parallel to the pathway
CC that governs SinR derepression (PubMed:19363116, PubMed:23646920).
CC Required for endosporulation (PubMed:23396918).
CC {ECO:0000269|PubMed:19363116, ECO:0000269|PubMed:23396918,
CC ECO:0000269|PubMed:23646920}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the forespore during sporulation.
CC {ECO:0000269|PubMed:23396918}.
CC -!- DISRUPTION PHENOTYPE: Mutation impairs pellicle formation, complex
CC colony architecture and motility inhibition in a sinR mutant background
CC (PubMed:19363116). Mutant is defective in biofilm formation and also
CC shows a delay in spore formation during biofilm formation
CC (PubMed:23396918). {ECO:0000269|PubMed:19363116,
CC ECO:0000269|PubMed:23396918}.
CC -!- SIMILARITY: Belongs to the RemA family. {ECO:0000255|HAMAP-
CC Rule:MF_01503, ECO:0000305}.
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DR EMBL; AL009126; CAE01452.1; -; Genomic_DNA.
DR RefSeq; NP_570902.1; NC_000964.3.
DR RefSeq; WP_003154355.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; Q7WY72; -.
DR SMR; Q7WY72; -.
DR STRING; 224308.BSU15670; -.
DR PaxDb; Q7WY72; -.
DR PRIDE; Q7WY72; -.
DR DNASU; 937956; -.
DR EnsemblBacteria; CAE01452; CAE01452; BSU_15670.
DR GeneID; 64303458; -.
DR GeneID; 66326444; -.
DR GeneID; 937956; -.
DR KEGG; bsu:BSU15670; -.
DR PATRIC; fig|224308.179.peg.1707; -.
DR eggNOG; COG2052; Bacteria.
DR InParanoid; Q7WY72; -.
DR OMA; RDRNMLI; -.
DR PhylomeDB; Q7WY72; -.
DR BioCyc; BSUB:BSU15670-MON; -.
DR PRO; PR:Q7WY72; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR HAMAP; MF_01503; RemA; 1.
DR InterPro; IPR007169; RemA-like.
DR PANTHER; PTHR38449; PTHR38449; 1.
DR Pfam; PF04025; DUF370; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..89
FT /note="Extracellular matrix regulatory protein A"
FT /id="PRO_0000050223"
SQ SEQUENCE 89 AA; 9849 MW; CF8A7A046F4463C2 CRC64;
MTIKLINIGF GNIISANRMI SIVSPESAPI KRMIQDARDR GMLIDATYGR RTRAVVVMDS
DHIILSAVQP ETVAHRLSVK EEIMDEGQG
