ID   REN_TGMVY               Reviewed;         132 AA.
AC   P03563;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 64.
DE   RecName: Full=Replication enhancer protein;
DE            Short=REn;
DE   AltName: Full=Protein AC3;
DE   AltName: Full=Protein AL3;
GN   ORFNames=AC3, AL3;
OS   Tomato golden mosaic virus (strain Yellow vein) (TGMV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=223341;
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16453557; DOI=10.1002/j.1460-2075.1984.tb02114.x;
RA   Hamilton W.D.O., Stein V.E., Coutts R.H.A., Buck K.W.;
RT   "Complete nucleotide sequence of the infectious cloned DNA components of
RT   tomato golden mosaic virus: potential coding regions and regulatory
RT   sequences.";
RL   EMBO J. 3:2197-2205(1984).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=8030214; DOI=10.1006/viro.1994.1442;
RA   Pedersen T.J., Hanley-Bowdoin L.;
RT   "Molecular characterization of the AL3 protein encoded by a bipartite
RT   geminivirus.";
RL   Virology 202:1070-1075(1994).
RN   [3]
RP   SUBUNIT, AND INTERACTION WITH THE REPLICATION-ASSOCIATED PROTEIN.
RX   PubMed=8794317; DOI=10.1128/jvi.70.10.6790-6795.1996;
RA   Settlage S.B., Miller A.B., Hanley-Bowdoin L.;
RT   "Interactions between geminivirus replication proteins.";
RL   J. Virol. 70:6790-6795(1996).
RN   [4]
RP   INTERACTION WITH THE REPLICATION-ASSOCIATED PROTEIN, AND INTERACTION WITH
RP   ZEA MAYS RBR1.
RX   PubMed=11162812; DOI=10.1006/viro.2000.0719;
RA   Settlage S.B., Miller A.B., Gruissem W., Hanley-Bowdoin L.;
RT   "Dual interaction of a geminivirus replication accessory factor with a
RT   viral replication protein and a plant cell cycle regulator.";
RL   Virology 279:570-576(2001).
RN   [5]
RP   MUTAGENESIS OF 28-TYR--LYS-30; 48-GLN--ASN-54; 65-PHE--SER-74;
RP   84-ASN--LYS-87 AND 91-MET--GLU-95.
RC   STRAIN=Isolate Dominican Republic;
RX   PubMed=16014949; DOI=10.1128/jvi.79.15.9885-9895.2005;
RA   Settlage S.B., See R.G., Hanley-Bowdoin L.;
RT   "Geminivirus C3 protein: replication enhancement and protein
RT   interactions.";
RL   J. Virol. 79:9885-9895(2005).
CC   -!- FUNCTION: Increases viral DNA accumulation. Enhances infectivity and
CC       symptom expression (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homooligomer. Interacts with the replication-associated
CC       protein (REP). Interacts with host proliferating cell nuclear antigen
CC       (PCNA). Interacts with host retinoblastoma-related protein 1 (RBR1),
CC       and may thereby deregulate the host cell cycle. Oligomerization and
CC       interaction with PCNA are necessary for optimal replication enhancement
CC       (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the geminiviridae replication enhancer protein
CC       family. {ECO:0000305}.
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DR   EMBL; K02029; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A04166; QQCVL3.
DR   Proteomes; UP000007405; Genome.
DR   GO; GO:0039684; P:rolling circle single-stranded viral DNA replication; IDA:UniProtKB.
DR   InterPro; IPR000657; Gemini_AL3.
DR   Pfam; PF01407; Gemini_AL3; 1.
DR   PRINTS; PR00231; GEMCOATAL3.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Reference proteome.
FT   CHAIN           1..132
FT                   /note="Replication enhancer protein"
FT                   /id="PRO_0000222245"
FT   MUTAGEN         28..30
FT                   /note="YFK->AAA: Severely reduced levels of viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         48..54
FT                   /note="QIRFNHN->AAAAAAA: No detectable levels of viral
FT                   DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         65..74
FT                   /note="FLNFQVWTTS->ALNAARIAA: Severely reduced levels of
FT                   viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         84..87
FT                   /note="NRFK->AAAA: Severely reduced levels of viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         91..95
FT                   /note="MLYLE->AAAAA: No detectable levels of viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
SQ   SEQUENCE   132 AA;  15695 MW;  37B7F12A15A682F1 CRC64;
     MDSRTGEPIT VPQAENGVYI WEITNPLYFK IISVEDPLYT NTRIYHLQIR FNHNLRRALD
     LHKAFLNFQV WTTSTTASGR TYLNRFKYLV MLYLEQLGVI CINNVIRAVR FATDRSYITH
     VLENHSIKYK FY