ID   REN_TYLCI               Reviewed;         134 AA.
AC   P27265;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   29-SEP-2021, entry version 53.
DE   RecName: Full=Replication enhancer protein;
DE            Short=REn;
DE   AltName: Full=15.6 kDa protein;
DE   AltName: Full=Protein C3;
DE   AltName: Full=Protein L3;
GN   ORFNames=C3, L3;
OS   Tomato yellow leaf curl virus (strain Israel) (TYLCV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=66366;
OH   NCBI_TaxID=185024; Cynanchum acutum.
OH   NCBI_TaxID=145753; Malva parviflora (Little mallow) (Cheeseweed mallow).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1926771; DOI=10.1016/0042-6822(91)90763-2;
RA   Navot N., Pichersky E., Zeidan M., Zamir D., Czosnek H.;
RT   "Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a
RT   single genomic component.";
RL   Virology 185:151-161(1991).
RN   [2]
RP   CHARACTERIZATION, INTERACTION WITH THE REPLICATION-ASSOCIATED PROTEIN,
RP   INTERACTION WITH SOLANUM LYCOPERSICUM PCNA, INTERACTION WITH ZEA MAYS RBR1,
RP   AND MUTAGENESIS OF 28-TYR--LYS-30; 49-GLN--ASN-55; 66-PHE--THR-75;
RP   84-ARG--ARG-87 AND 91-LEU--ASP-95.
RC   STRAIN=Isolate Dominican Republic;
RX   PubMed=16014949; DOI=10.1128/jvi.79.15.9885-9895.2005;
RA   Settlage S.B., See R.G., Hanley-Bowdoin L.;
RT   "Geminivirus C3 protein: replication enhancement and protein
RT   interactions.";
RL   J. Virol. 79:9885-9895(2005).
CC   -!- FUNCTION: Increases viral DNA accumulation. Enhances infectivity and
CC       symptom expression (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homooligomer. Interacts with the replication-associated
CC       protein (REP). Interacts with host proliferating cell nuclear antigen
CC       (PCNA). Interacts with host retinoblastoma-related protein 1 (RBR1),
CC       and may thereby deregulate the host cell cycle. Oligomerization and
CC       interaction with PCNA are necessary for optimal replication
CC       enhancement. {ECO:0000269|PubMed:16014949}.
CC   -!- SIMILARITY: Belongs to the geminiviridae replication enhancer protein
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X15656; CAA33690.1; -; Genomic_DNA.
DR   PIR; B40779; QQCVC3.
DR   Proteomes; UP000007547; Genome.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR000657; Gemini_AL3.
DR   Pfam; PF01407; Gemini_AL3; 1.
DR   PRINTS; PR00231; GEMCOATAL3.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Reference proteome.
FT   CHAIN           1..134
FT                   /note="Replication enhancer protein"
FT                   /id="PRO_0000222247"
FT   MUTAGEN         28..30
FT                   /note="YFK->AAA: Severely reduced levels of viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         49..55
FT                   /note="QIRFNHN->AAAAAAA: Severely reduced levels of viral
FT                   DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         66..75
FT                   /note="FLDFRIWTTL->ALNAARIAA: Severely reduced levels of
FT                   viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         84..87
FT                   /note="RVFR->AAAA: Severely reduced levels of viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
FT   MUTAGEN         91..95
FT                   /note="LKYLD->AAAAA: Severely reduced levels of viral DNA."
FT                   /evidence="ECO:0000269|PubMed:16014949"
SQ   SEQUENCE   134 AA;  15939 MW;  ED46E0012519808F CRC64;
     MDSRTGELIT APQAENGVFI WEINNPLYFK ITEHSQRPFL MNHDIISIQI RFNHNIRKVM
     GIHKCFLDFR IWTTLQPQTG HFLRVFRYEV LKYLDSLGVI SINNVIRAVD HVLYDVLENT
     INVTETHDIK YKFY