ID   REP12_ECOLX             Reviewed;         325 AA.
AC   P0A254; Q57481; Q99371;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=RepFIB replication protein A;
GN   Name=repA; Synonyms=repI;
OS   Escherichia coli.
OG   Plasmid IncFI ColV3-K30.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2703470; DOI=10.1128/jb.171.4.2195-2201.1989;
RA   Perez-Casal J.F., Gammie A.E., Crosa J.H.;
RT   "Nucleotide sequence analysis and expression of the minimum REPI
RT   replication region and incompatibility determinants of pColV-K30.";
RL   J. Bacteriol. 171:2195-2201(1989).
RN   [2]
RP   ERRATUM OF PUBMED:2703470.
RA   Perez-Casal J.F., Gammie A.E., Crosa J.H.;
RL   J. Bacteriol. 173:2409-2409(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8356112; DOI=10.1006/plas.1993.1020;
RA   Gibbs M.D., Spiers A.J., Bergquist P.L.;
RT   "RepFIB: a basic replicon of large plasmids.";
RL   Plasmid 29:165-179(1993).
CC   -!- FUNCTION: This protein is essential for plasmid replication; it is
CC       involved in copy control functions. In vitro, binds to the DNA repeat
CC       units, BCDD'D'', EFG and HIJ (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the initiator RepB protein family.
CC       {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-25 is the initiator.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA23197.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M24908; AAA23197.1; ALT_INIT; Genomic_DNA.
DR   EMBL; L01251; AAA71881.1; -; Unassigned_DNA.
DR   PIR; I40787; I40787.
DR   RefSeq; NP_863021.1; NC_004998.1.
DR   RefSeq; WP_000361611.1; NZ_WWEL01000079.1.
DR   RefSeq; YP_001816634.1; NC_010558.1.
DR   RefSeq; YP_002527466.1; NC_011964.1.
DR   RefSeq; YP_009068209.1; NC_025139.1.
DR   RefSeq; YP_009070678.1; NC_025175.1.
DR   RefSeq; YP_009071151.1; NC_025179.1.
DR   RefSeq; YP_444069.1; NC_007675.1.
DR   AlphaFoldDB; P0A254; -.
DR   OMA; MTLTPNN; -.
DR   OrthoDB; 810260at2; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000525; Initiator_Rep_prot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF01051; Rep_3; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; Plasmid; Plasmid copy control.
FT   CHAIN           1..325
FT                   /note="RepFIB replication protein A"
FT                   /id="PRO_0000068307"
FT   REGION          279..298
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   325 AA;  37315 MW;  B7A4B6361A9327DB CRC64;
     MDKSSGELVT LTPNNNNTVQ PVALMRLGVF VPTLKSLKNS KKNTLSRTDA TEELTRLSLA
     RAEGFDKVEI TGPRLDMDND FKTWVGIIHS FARHNVIGDK VELPFVEFAK LCGIPSSQSS
     RRLRERISPS LKRIAGTVIS FSRTDEKHTR EYITHLVQSA YYDTERDIVQ LQADPRLFEL
     YQFDRKVLLQ LKAINALKRR ESAQALYTFI ESLPRDPAPI SLARLRARLN LKSPVFSQNQ
     TVRRAMEQLR EIGYLDYTEI QRGRTKFFCI HYRRPRLKAP NDESKENPLQ PSPAEKVSPE
     MAEKLALLEK LGITLDDLEK LFKSR