ID   REP5_ECOLX              Reviewed;         286 AA.
AC   P24042;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Replication protein RepA;
GN   Name=repA;
OS   Escherichia coli.
OG   Plasmid P1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RX   PubMed=3949778; DOI=10.1016/s0021-9258(17)35681-8;
RA   Abeles A.L.;
RT   "P1 plasmid replication. Purification and DNA-binding activity of the
RT   replication protein RepA.";
RL   J. Biol. Chem. 261:3548-3555(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2198259; DOI=10.1128/jb.172.8.4441-4447.1990;
RA   Muraiso K., Mukhopadhyay G., Chattoraj D.K.;
RT   "Location of a P1 plasmid replication inhibitor determinant within the
RT   initiator gene.";
RL   J. Bacteriol. 172:4441-4447(1990).
CC   -!- FUNCTION: RepA is essential for origin function, autoregulates its own
CC       synthesis from the promoter, and, when overproduced, blocks origin
CC       function.
CC   -!- SIMILARITY: Belongs to the initiator RepB protein family.
CC       {ECO:0000305}.
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DR   EMBL; K02380; AAA99229.1; -; Genomic_DNA.
DR   PIR; A37764; A37764.
DR   RefSeq; WP_001076427.1; NZ_WWEV01000077.1.
DR   AlphaFoldDB; P24042; -.
DR   SMR; P24042; -.
DR   OrthoDB; 810260at2; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR000525; Initiator_Rep_prot.
DR   Pfam; PF01051; Rep_3; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA replication; DNA-binding; Plasmid.
FT   CHAIN           1..286
FT                   /note="Replication protein RepA"
FT                   /id="PRO_0000068301"
SQ   SEQUENCE   286 AA;  32220 MW;  169585F585B788B4 CRC64;
     MNQSFISDIL YADIESKAKE LTVNSNNTVQ PVALMRLGVF VPKPSKSKGE SKEIDATKAF
     SQLEIAKAEG YDDIKITGPR LDMDTDFKTW IGVIYAFSKY GLSSNTIQLS FQEFAKACGF
     PSKRLDAKLR LTIHESLGRL RNKGIAFKRG KDAKGGYQTG LLKVGRFDAD LDLIELEADS
     KLWELFQLDY RVLLQHHALR ALPKKEAAQA IYTFIESLPQ NPLPLSFARI RERLALQSAV
     GEQNRIIKKA IEQLKTIGYL DCSIEKKGRE SFVIVHSRNP KLKLPE