ID   REP7_ECOLX              Reviewed;         343 AA.
AC   Q52221;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Replication initiation protein;
DE   AltName: Full=Replication-associated protein;
GN   Name=repA;
OS   Escherichia coli.
OG   Plasmid IncB pMu707.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1706708; DOI=10.1128/jb.173.7.2393-2397.1991;
RA   Praszkier J., Wei T., Siemering K., Pittard J.;
RT   "Comparative analysis of the replication regions of IncB, IncK, and IncZ
RT   plasmids.";
RL   J. Bacteriol. 173:2393-2397(1991).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=12644491; DOI=10.1128/jb.185.7.2210-2218.2003;
RA   Betteridge T., Yang J., Pittard A.J., Praszkier J.;
RT   "Interaction of the initiator protein of an IncB plasmid with its origin of
RT   DNA replication.";
RL   J. Bacteriol. 185:2210-2218(2003).
CC   -!- FUNCTION: Binds specifically to the motif 5'-AANCYGCAA-3' (repA box) in
CC       the origin of replication. Acts preferentially in cis; binding to the
CC       repA boxes is sequential, with the boxes closest to the DnaA binding
CC       site being occupied first. It is involved in promoting open complex
CC       formation by unwinding the oriB at sequence 5'-TCTTAA-3'.
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DR   EMBL; M93062; AAA98176.1; -; Genomic_DNA.
DR   PIR; B42382; B42382.
DR   RefSeq; WP_000907880.1; NZ_VTAZ01000019.1.
DR   RefSeq; YP_002756659.1; NC_012487.1.
DR   AlphaFoldDB; Q52221; -.
DR   PRIDE; Q52221; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0006276; P:plasmid maintenance; IEA:InterPro.
DR   InterPro; IPR003446; Plasmid_replication_init_RepA.
DR   Pfam; PF02387; IncFII_repA; 1.
PE   1: Evidence at protein level;
KW   DNA replication; DNA-binding; Plasmid.
FT   CHAIN           1..343
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068338"
FT   REGION          42..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   343 AA;  40499 MW;  9D6A51FADCBB1532 CRC64;
     MAGLKNTSYN AVHWSQLAPE EQIRFWEDYE AGRATTFLVE PERKRTKRRR GEHSTKPKCE
     NPSWYRPERY KALKGQLGHA YNRLVKKDPV TGEQSLRMRM SRHPFYVQKR TFVGRKYAFR
     PEKQRLLDAI WPVLVSFSDA GTHTVGMSVT RLAEEISPKD SEGHVIPELE VTVSRLSRLL
     AEQVRFGVLG VSEETMWDRE HRQRLPRYVW ITPAGWQMLG VDMVKLHEQQ QKRLRESEIR
     QQLIREGVLR EDEDISVHAA RKRWYLQRSQ DALKKRREKA AASKRANRLK KLPVDQQIYE
     MAEYLRKRLP PDEAYFCSDD HLKRLAIREL RQLELTLAAP PPH