REPA2_ECOLX
ID REPA2_ECOLX Reviewed; 285 AA.
AC P03066; Q47411;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Replication initiation protein;
GN Name=repA; Synonyms=repA1;
OS Escherichia coli.
OG Plasmid IncFII R100 (NR1), and Plasmid IncFII R1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=7003300; DOI=10.1007/bf00271742;
RA Rosen J., Ryder T., Inokuchi H., Ohtsubo H., Ohtsubo E.;
RT "Genes and sites involved in replication and incompatibility of an R100
RT plasmid derivative based on nucleotide sequence analysis.";
RL Mol. Gen. Genet. 179:527-537(1980).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
RC PLASMID=IncFII R1, and IncFII R100 (NR1);
RX PubMed=6163994; DOI=10.1038/290794a0;
RA Rosen J., Ryder T., Ohtsubo H., Ohtsubo E.;
RT "Role of RNA transcripts in replication incompatibility and copy number
RT control in antibiotic resistance plasmid derivatives.";
RL Nature 290:794-797(1981).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 270-285.
RC PLASMID=IncFII R1;
RX PubMed=3041379; DOI=10.1093/nar/16.14.6493;
RA Masai H., Arai K.;
RT "RepA protein- and oriR-dependent initiation of R1 plasmid replication:
RT identification of a rho-dependent transcription terminator required for
RT cis-action of repA protein.";
RL Nucleic Acids Res. 16:6493-6514(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=3050127; DOI=10.1016/0022-2836(88)90281-1;
RA Dong X., Womble D.D., Rownd R.H.;
RT "In-vivo studies on the cis-acting replication initiator protein of IncFII
RT plasmid NR1.";
RL J. Mol. Biol. 202:495-509(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=2580099; DOI=10.1016/0022-2836(85)90228-1;
RA Womble D.D., Sampathkumar P., Easton A.M., Luckow V.A., Rownd R.H.;
RT "Transcription of the replication control region of the IncFII R-plasmid
RT NR1 in vitro and in vivo.";
RL J. Mol. Biol. 181:395-410(1985).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=3019092; DOI=10.1016/0065-227x(86)90018-3;
RA Ohtsubo H., Ryder T.B., Maeda Y., Armstrong K., Ohtsubo E.;
RT "DNA replication of the resistance plasmid R100 and its control.";
RL Adv. Biophys. 21:115-133(1986).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
RC PLASMID=IncFII R100 (NR1);
RX PubMed=2445727; DOI=10.1128/jb.169.12.5353-5363.1987;
RA Dong X.N., Womble D.D., Rownd R.H.;
RT "Transcriptional pausing in a region important for plasmid NR1 replication
RT control.";
RL J. Bacteriol. 169:5353-5363(1987).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 252-285.
RC PLASMID=IncFII R100 (NR1);
RX PubMed=8396115; DOI=10.1128/jb.175.17.5350-5358.1993;
RA Jiang T., Min Y.-N., Liu W., Womble D.D., Rownd R.H.;
RT "Insertion and deletion mutations in the repA4 region of the IncFII plasmid
RT NR1 cause unstable inheritance.";
RL J. Bacteriol. 175:5350-5358(1993).
CC -!- FUNCTION: This protein is essential for plasmid replication; it is
CC involved in copy control functions.
CC -!- SIMILARITY: Belongs to the IncFII RepA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA26169.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J01762; AAA92257.1; -; Genomic_DNA.
DR EMBL; J01770; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; X12587; CAA31100.1; -; Genomic_DNA.
DR EMBL; X12776; CAA31263.1; -; Genomic_DNA.
DR EMBL; X02302; CAA26168.1; -; Genomic_DNA.
DR EMBL; X02302; CAA26169.1; ALT_INIT; Genomic_DNA.
DR EMBL; M26840; AAA26067.1; -; Genomic_DNA.
DR PIR; A03602; IDECRP.
DR PIR; I64780; I64780.
DR RefSeq; NP_957643.1; NC_005327.1.
DR RefSeq; WP_000130646.1; NZ_WIOY01000011.1.
DR RefSeq; YP_001096511.1; NC_009133.1.
DR RefSeq; YP_004869998.1; NC_016039.1.
DR RefSeq; YP_006952184.1; NC_019057.1.
DR RefSeq; YP_006953267.1; NC_019071.1.
DR RefSeq; YP_006953365.1; NC_019072.1.
DR RefSeq; YP_006953887.1; NC_019090.1.
DR RefSeq; YP_006954223.1; NC_019095.1.
DR AlphaFoldDB; P03066; -.
DR GeneID; 58462573; -.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR InterPro; IPR017837; Plasmid_rep_init_RepA_IncFII.
DR InterPro; IPR003446; Plasmid_replication_init_RepA.
DR Pfam; PF02387; IncFII_repA; 1.
DR TIGRFAMs; TIGR03474; incFII_RepA; 1.
PE 3: Inferred from homology;
KW DNA replication; Plasmid; Plasmid copy control.
FT CHAIN 1..285
FT /note="Replication initiation protein"
FT /id="PRO_0000216226"
FT CONFLICT 55
FT /note="R -> H (in Ref. 6; AAA26067)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 285 AA; 32756 MW; A21C9D59D24B26BB CRC64;
MTDLHQTYYR QVKNPNPVFT PREGAGTLKF CEKLMEKAVG FTSRFDFAIH VAHARSRGLR
RRMPPVLRRR AIDALLQGLC FHYDPLANRV QCSITTLAIE CGLATESAAG KLSITRATRA
LTFLSELGLI TYQTEYDPLI GCYIPTDITF TSALFAALDV SEEAVAAARR SRVVWENKQR
KKQGLDTLGM DELIAKAWRF VRERFRSYQT ELKSRGIKRA RARRDADRER QDIVTLVKRQ
LTREIAEGRF TANREAVKRE VERRVKERMI LSRNRNYSRL ATASP
