ID   REPA_MISV9              Reviewed;         217 AA.
AC   Q67591;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Replication-associated protein A;
DE            Short=RepA;
DE            EC=3.1.21.-;
GN   ORFNames=C1;
OS   Miscanthus streak virus (isolate 91) (MiSV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Mastrevirus.
OX   NCBI_TaxID=268776;
OH   NCBI_TaxID=183675; Miscanthus sacchariflorus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1919519; DOI=10.1099/0022-1317-72-10-2325;
RA   Chatani M., Matsumoto Y., Mizuta H., Ikegami M., Boulton M.I., Davies J.W.;
RT   "The nucleotide sequence and genome structure of the geminivirus miscanthus
RT   streak virus.";
RL   J. Gen. Virol. 72:2325-2331(1991).
CC   -!- FUNCTION: Implicated in enhancement of V-sense gene expression. Acts a
CC       an inhibitor of C-sense gene transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homooligomer. Part of the C- and V-complexes which are RepA-
CC       Rep-DNA complexes involved in the c-sense and v-sense transcription (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=RepA;
CC         IsoId=Q67591-1; Sequence=Displayed;
CC       Name=Rep;
CC         IsoId=Q67590-1; Sequence=External;
CC   -!- DOMAIN: There are 3 rolling circle replication (RCR) motifs. RCR-2 may
CC       be involved in metal coordination. RCR-3 is required for phosphodiester
CC       bond cleavage for initiation of RCR.
CC   -!- MISCELLANEOUS: [Isoform RepA]: Produced from the unspliced transcript.
CC   -!- SIMILARITY: Belongs to the geminiviridae Rep protein family.
CC       {ECO:0000305}.
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DR   EMBL; D01030; BAA00835.1; -; Genomic_DNA.
DR   PIR; JQ1358; JQ1358.
DR   RefSeq; NP_569147.1; NC_003379.1. [Q67591-1]
DR   SMR; Q67591; -.
DR   PRIDE; Q67591; -.
DR   GeneID; 932215; -.
DR   KEGG; vg:932215; -.
DR   Proteomes; UP000008874; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016888; F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
DR   InterPro; IPR001301; Gemini_AL1_CLV.
DR   InterPro; IPR001191; Gemini_AL1_REP.
DR   InterPro; IPR022690; Gemini_AL1_REP_cat-dom.
DR   InterPro; IPR022692; Gemini_AL1_REP_central.
DR   Pfam; PF00799; Gemini_AL1; 1.
DR   Pfam; PF08283; Gemini_AL1_M; 1.
DR   PRINTS; PR00227; GEMCOATAL1.
DR   PRINTS; PR00228; GEMCOATCLVL1.
PE   3: Inferred from homology;
KW   Activator; Alternative splicing; DNA-binding; Endonuclease;
KW   G1/S host cell cycle checkpoint dysregulation by virus; Host cytoplasm;
KW   Host nucleus; Host-virus interaction; Hydrolase; Metal-binding;
KW   Modulation of host cell cycle by virus; Nuclease; Reference proteome;
KW   Repressor.
FT   CHAIN           1..217
FT                   /note="Replication-associated protein A"
FT                   /id="PRO_0000316941"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          176..188
FT                   /note="Oligomerization"
FT                   /evidence="ECO:0000250"
FT   MOTIF           29..33
FT                   /note="RCR-1"
FT   MOTIF           71..76
FT                   /note="RCR-2"
FT   MOTIF           111..114
FT                   /note="RCR-3"
FT   ACT_SITE        111
FT                   /note="For DNA cleavage activity"
FT                   /evidence="ECO:0000250"
FT   BINDING         63
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         71
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         73
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   217 AA;  25050 MW;  E511C43503BFB39E CRC64;
     MRAPASSAAS NRPGPSNHPT PRWNSKQFFL TYPHCNLTPS ELMKELFSRL TEKIPGYIKV
     SQEFHKDGDP HLHVLIQLNT KLCTRNPKFF DVQGFHPNIQ PVRDAEKVFG YISKTNGDSD
     EMGELQLRIK KPEKPTRDQR MAMIIASSTN RNEYLSMVRK EFPFDWAIRL QQFEYSAAAL
     FTEPPPVYQS PFPNEQIVCP PELVDIIDQE WNQVTTD