REPA_MSVN
ID REPA_MSVN Reviewed; 272 AA.
AC P14980;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Replication-associated protein A;
DE Short=RepA;
DE EC=3.1.21.-;
GN ORFNames=C1;
OS Maize streak virus genotype A (isolate Nigeria) (MSV).
OC Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC Geplafuvirales; Geminiviridae; Mastrevirus.
OX NCBI_TaxID=10823;
OH NCBI_TaxID=4498; Avena sativa (Oat).
OH NCBI_TaxID=217170; Axonopus compressus.
OH NCBI_TaxID=240436; Brachiaria deflexa.
OH NCBI_TaxID=4543; Cenchrus americanus (Pearl millet) (Pennisetum glaucum).
OH NCBI_TaxID=281129; Cenchrus polystachios.
OH NCBI_TaxID=4505; Coix lacryma-jobi (Job's tears).
OH NCBI_TaxID=270102; Dactyloctenium aegyptium.
OH NCBI_TaxID=66017; Digitaria.
OH NCBI_TaxID=90396; Echinochloa colona.
OH NCBI_TaxID=4511; Eleusine coracana (Indian finger millet) (Ragi).
OH NCBI_TaxID=29674; Eleusine indica (Goosegrass) (Cynosurus indicus).
OH NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH NCBI_TaxID=59788; Megathyrsus maximus.
OH NCBI_TaxID=29709; Melinis repens (Natal redtop) (Rhynchelytrum repens).
OH NCBI_TaxID=4538; Oryza glaberrima (African rice).
OH NCBI_TaxID=4530; Oryza sativa (Rice).
OH NCBI_TaxID=158143; Paspalum conjugatum (Hilo grass).
OH NCBI_TaxID=147272; Paspalum notatum (Bahia grass).
OH NCBI_TaxID=173849; Paspalum scrobiculatum.
OH NCBI_TaxID=300125; Rottboellia cochinchinensis.
OH NCBI_TaxID=4547; Saccharum officinarum (Sugarcane).
OH NCBI_TaxID=192628; Setaria barbata.
OH NCBI_TaxID=4565; Triticum aestivum (Wheat).
OH NCBI_TaxID=4577; Zea mays (Maize).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6526009; DOI=10.1002/j.1460-2075.1984.tb02258.x;
RA Mullineaux P.M., Donson J., Morris-Krsinich B.A.M., Boulton M.I.,
RA Davies J.W.;
RT "The nucleotide sequence of maize streak virus DNA.";
RL EMBO J. 3:3063-3068(1984).
RN [2]
RP REGION OF TRANSACTIVATION, REGION OF OLIGOMERIZATION, AND INTERACTION WITH
RP ZEA MAYS RBR1.
RX PubMed=9862488; DOI=10.1023/a:1006076316887;
RA Horvath G.V., Pettko-Szandtner A., Nikovics K., Bilgin M., Boulton M.I.,
RA Davies J.W., Gutierrez C., Dudits D.;
RT "Prediction of functional regions of the maize streak virus replication-
RT associated proteins by protein-protein interaction analysis.";
RL Plant Mol. Biol. 38:699-712(1998).
CC -!- FUNCTION: Implicated in enhancement of V-sense gene expression. Acts a
CC an inhibitor of C-sense gene transcription (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homooligomer. Interacts with host retinoblastoma-related
CC protein 1 (RBR1), and may thereby deregulate the host cell cycle. Part
CC of the C- and V-complexes which are RepA-Rep-DNA complexes involved in
CC the c-sense and v-sense transcription (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=RepA;
CC IsoId=P14980-1; Sequence=Displayed;
CC Name=Rep;
CC IsoId=P14978-1; Sequence=External;
CC -!- DOMAIN: There are 3 rolling circle replication (RCR) motifs. RCR-2 may
CC be involved in metal coordination. RCR-3 is required for phosphodiester
CC bond cleavage for initiation of RCR.
CC -!- MISCELLANEOUS: [Isoform RepA]: Produced from the unspliced transcript.
CC -!- SIMILARITY: Belongs to the geminiviridae Rep protein family.
CC {ECO:0000305}.
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DR EMBL; X01633; CAA25793.1; -; Genomic_DNA.
DR PIR; A05158; A05158.
DR SMR; P14980; -.
DR Proteomes; UP000007779; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016888; F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
DR InterPro; IPR001146; Gemini_AL1_MSV.
DR InterPro; IPR001191; Gemini_AL1_REP.
DR InterPro; IPR022690; Gemini_AL1_REP_cat-dom.
DR InterPro; IPR022692; Gemini_AL1_REP_central.
DR Pfam; PF00799; Gemini_AL1; 1.
DR Pfam; PF08283; Gemini_AL1_M; 1.
DR PRINTS; PR00227; GEMCOATAL1.
DR PRINTS; PR00229; GEMCOATMSVL1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Endonuclease;
KW G1/S host cell cycle checkpoint dysregulation by virus; Host cytoplasm;
KW Host nucleus; Host-virus interaction; Hydrolase; Metal-binding;
KW Modulation of host cell cycle by virus; Nuclease; Reference proteome;
KW Repressor.
FT CHAIN 1..272
FT /note="Replication-associated protein A"
FT /id="PRO_0000222207"
FT REGION 175..187
FT /note="Oligomerization"
FT REGION 198..202
FT /note="Binding to RBR1"
FT REGION 221..230
FT /note="Transactivation"
FT REGION 245..272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 18..22
FT /note="RCR-1"
FT MOTIF 60..65
FT /note="RCR-2"
FT MOTIF 100..103
FT /note="RCR-3"
FT COMPBIAS 245..265
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 100
FT /note="For DNA cleavage activity"
FT /evidence="ECO:0000250"
FT BINDING 52
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 60
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 62
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 104
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
SQ SEQUENCE 272 AA; 31387 MW; 3633DF3EDDCEED39 CRC64;
MASSSSNRQF SHRNANTFLT YPKCPENPEI ACQMIWELVV RWIPKYILCA REAHKDGSLH
LHALLQTEKP VRISDSRFFD INGFHPNIQS AKSVNRVRDY ILKEPLAVFE RGTFIPRKSP
FLGKSDSEVK EKKPSKDEIM RDIISHATSK EEYLSMIQKE LPFDWSTKLQ YFEYSANKLF
PEIQEEFTNP HPPSSPDLLC NESINDWLQP NIFQVSPEAY MLLQPTCYTL EDAISDLQWM
DSVSSHQMKD QESRASTSSA QQEPENLLGP EA
