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REPA_MSVRA
ID   REPA_MSVRA              Reviewed;         272 AA.
AC   Q91MF7; Q9YPX9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Replication-associated protein A;
DE            Short=RepA;
DE            EC=3.1.21.-;
GN   ORFNames=C1;
OS   Maize streak virus genotype D (isolate Raw) (MSV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Mastrevirus.
OX   NCBI_TaxID=268343;
OH   NCBI_TaxID=4498; Avena sativa (Oat).
OH   NCBI_TaxID=217170; Axonopus compressus.
OH   NCBI_TaxID=240436; Brachiaria deflexa.
OH   NCBI_TaxID=4543; Cenchrus americanus (Pearl millet) (Pennisetum glaucum).
OH   NCBI_TaxID=281129; Cenchrus polystachios.
OH   NCBI_TaxID=4505; Coix lacryma-jobi (Job's tears).
OH   NCBI_TaxID=270102; Dactyloctenium aegyptium.
OH   NCBI_TaxID=66017; Digitaria.
OH   NCBI_TaxID=90396; Echinochloa colona.
OH   NCBI_TaxID=4511; Eleusine coracana (Indian finger millet) (Ragi).
OH   NCBI_TaxID=29674; Eleusine indica (Goosegrass) (Cynosurus indicus).
OH   NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH   NCBI_TaxID=59788; Megathyrsus maximus.
OH   NCBI_TaxID=29709; Melinis repens (Natal redtop) (Rhynchelytrum repens).
OH   NCBI_TaxID=4538; Oryza glaberrima (African rice).
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
OH   NCBI_TaxID=158143; Paspalum conjugatum (Hilo grass).
OH   NCBI_TaxID=147272; Paspalum notatum (Bahia grass).
OH   NCBI_TaxID=173849; Paspalum scrobiculatum.
OH   NCBI_TaxID=300125; Rottboellia cochinchinensis.
OH   NCBI_TaxID=4547; Saccharum officinarum (Sugarcane).
OH   NCBI_TaxID=192628; Setaria barbata.
OH   NCBI_TaxID=4565; Triticum aestivum (Wheat).
OH   NCBI_TaxID=4577; Zea mays (Maize).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11601896; DOI=10.1006/viro.2001.1075;
RA   Martin D.P., Willment J.A., Billharz R., Velders R., Odhiambo B.,
RA   Njuguna J., James D., Rybicki E.P.;
RT   "Sequence diversity and virulence in Zea mays of Maize streak virus
RT   isolates.";
RL   Virology 288:247-255(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-261.
RX   PubMed=11311346; DOI=10.1016/s0166-0934(00)00299-8;
RA   Willment J.A., Martin D.P., Rybicki E.P.;
RT   "Analysis of the diversity of African streak mastreviruses using PCR-
RT   generated RFLPs and partial sequence data.";
RL   J. Virol. Methods 93:75-87(2001).
CC   -!- FUNCTION: Implicated in enhancement of V-sense gene expression. Acts a
CC       an inhibitor of C-sense gene transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homooligomer. Interacts with host retinoblastoma-related
CC       protein 1 (RBR1), and may thereby deregulate the host cell cycle. Part
CC       of the C- and V-complexes which are RepA-Rep-DNA complexes involved in
CC       the c-sense and v-sense transcription (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=RepA;
CC         IsoId=Q91MF7-1; Sequence=Displayed;
CC       Name=Rep;
CC         IsoId=Q91MF8-1; Sequence=External;
CC   -!- DOMAIN: There are 3 rolling circle replication (RCR) motifs. RCR-2 may
CC       be involved in metal coordination. RCR-3 is required for phosphodiester
CC       bond cleavage for initiation of RCR.
CC   -!- MISCELLANEOUS: [Isoform RepA]: Produced from the unspliced transcript.
CC   -!- SIMILARITY: Belongs to the geminiviridae Rep protein family.
CC       {ECO:0000305}.
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DR   EMBL; AF329889; AAK73473.1; -; Genomic_DNA.
DR   EMBL; AJ012639; CAA10092.1; -; Genomic_DNA.
DR   SMR; Q91MF7; -.
DR   Proteomes; UP000007781; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016888; F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
DR   InterPro; IPR001146; Gemini_AL1_MSV.
DR   InterPro; IPR001191; Gemini_AL1_REP.
DR   InterPro; IPR022690; Gemini_AL1_REP_cat-dom.
DR   InterPro; IPR022692; Gemini_AL1_REP_central.
DR   Pfam; PF00799; Gemini_AL1; 1.
DR   Pfam; PF08283; Gemini_AL1_M; 1.
DR   PRINTS; PR00227; GEMCOATAL1.
DR   PRINTS; PR00229; GEMCOATMSVL1.
PE   3: Inferred from homology;
KW   Activator; Alternative splicing; DNA-binding; Endonuclease;
KW   G1/S host cell cycle checkpoint dysregulation by virus; Host cytoplasm;
KW   Host nucleus; Host-virus interaction; Hydrolase; Metal-binding;
KW   Modulation of host cell cycle by virus; Nuclease; Reference proteome;
KW   Repressor.
FT   CHAIN           1..272
FT                   /note="Replication-associated protein A"
FT                   /id="PRO_0000316937"
FT   REGION          175..187
FT                   /note="Oligomerization"
FT                   /evidence="ECO:0000250"
FT   REGION          198..202
FT                   /note="Binding to RBR1"
FT                   /evidence="ECO:0000250"
FT   REGION          221..230
FT                   /note="Transactivation"
FT                   /evidence="ECO:0000250"
FT   REGION          250..272
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           18..22
FT                   /note="RCR-1"
FT   MOTIF           60..65
FT                   /note="RCR-2"
FT   MOTIF           100..103
FT                   /note="RCR-3"
FT   COMPBIAS        250..266
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        100
FT                   /note="For DNA cleavage activity"
FT                   /evidence="ECO:0000250"
FT   BINDING         52
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         60
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         62
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         104
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   272 AA;  31396 MW;  3FDABDBC38E798BB CRC64;
     MTSSSSNRQF LHRTANTFLT YPQCPEHPEI ISQRIWDLVG RWNPLYIICA QEAHEDGNMH
     LHALIQTDKQ VRTTDSRFFD IDGFHPNIQS AMSPNKVRDY ILKEPLALFE RGTFVPRKKT
     FLGNSSKGNS EKKPSKDEIM QDIISHATSK PEYLSMVRKS FPYDWATKLQ YFEYSANKLF
     PDIQEEFINP HPTSEPDLLC NESIKDWLQP NIYQVSPQAY MLLQPNCYTV DDAISDLQWL
     DDLTSKQIMD QESRASTSSV QQGQENLLGP EA
 
 
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