REPA_ORYSJ
ID REPA_ORYSJ Reviewed; 378 AA.
AC Q7GDU7; A3CA98; Q53LC6;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Cysteine endopeptidase RepA {ECO:0000303|PubMed:10394640};
DE EC=3.4.22.- {ECO:0000250|UniProtKB:Q7F3A8};
DE Flags: Precursor;
GN Name=REPA {ECO:0000303|PubMed:10394640};
GN OrderedLocusNames=Os11g0255300 {ECO:0000312|EMBL:BAT13463.1},
GN LOC_Os11g14900 {ECO:0000312|EMBL:ABA92412.1};
GN ORFNames=OsJ_33558 {ECO:0000312|EMBL:EAZ18011.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10394640; DOI=10.1093/oxfordjournals.pcp.a029565;
RA Kato H., Shintani A., Minamikawa T.;
RT "The structure and organization of two cysteine endopeptidase genes from
RT rice.";
RL Plant Cell Physiol. 40:462-467(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP DEVELOPMENTAL STAGE, AND INDUCTION BY GIBBERELLIN.
RX PubMed=9435140; DOI=10.1093/oxfordjournals.pcp.a029111;
RA Shintani A., Kato H., Minamikawa T.;
RT "Hormonal regulation of expression of two cysteine endopeptidase genes in
RT rice seedlings.";
RL Plant Cell Physiol. 38:1242-1248(1997).
CC -!- FUNCTION: Cysteine endopeptidase that digests in vitro both the acidic
CC and basic subunits of glutelin, the major seed storage protein of rice.
CC {ECO:0000250|UniProtKB:Q7F3A8}.
CC -!- SUBCELLULAR LOCATION: Protein storage vacuole
CC {ECO:0000250|UniProtKB:Q7F3A8}.
CC -!- DEVELOPMENTAL STAGE: Expressed in seedlings, from 6 to 21 days after
CC seed imbibition. {ECO:0000269|PubMed:9435140}.
CC -!- INDUCTION: Induced by treatment with gibberellin (GA3).
CC {ECO:0000269|PubMed:9435140}.
CC -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089,
CC ECO:0000255|PROSITE-ProRule:PRU10090}.
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DR EMBL; AB004648; BAA83472.1; -; Genomic_DNA.
DR EMBL; AC145324; AAX94791.1; -; Genomic_DNA.
DR EMBL; AP008217; BAF27993.1; -; Genomic_DNA.
DR EMBL; DP000010; ABA92412.1; -; Genomic_DNA.
DR EMBL; DP000010; ABA92413.1; -; Genomic_DNA.
DR EMBL; DP000010; ABA92414.1; -; Genomic_DNA.
DR EMBL; AP014967; BAT13463.1; -; Genomic_DNA.
DR EMBL; CM000148; EAZ18011.1; -; Genomic_DNA.
DR EMBL; AK071495; BAG92522.1; -; mRNA.
DR RefSeq; XP_015615655.1; XM_015760169.1.
DR RefSeq; XP_015615656.1; XM_015760170.1.
DR RefSeq; XP_015615657.1; XM_015760171.1.
DR RefSeq; XP_015615658.1; XM_015760172.1.
DR SMR; Q7GDU7; -.
DR STRING; 39947.Q7GDU7; -.
DR MEROPS; C01.168; -.
DR PRIDE; Q7GDU7; -.
DR EnsemblPlants; Os11t0255300-01; Os11t0255300-01; Os11g0255300.
DR GeneID; 4350216; -.
DR Gramene; Os11t0255300-01; Os11t0255300-01; Os11g0255300.
DR KEGG; osa:4350216; -.
DR eggNOG; KOG1543; Eukaryota.
DR HOGENOM; CLU_012184_1_0_1; -.
DR OMA; YPYEAKG; -.
DR OrthoDB; 1275401at2759; -.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000007752; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR CDD; cd02248; Peptidase_C1A; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR025661; Pept_asp_AS.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR025660; Pept_his_AS.
DR InterPro; IPR000668; Peptidase_C1A_C.
DR InterPro; IPR039417; Peptidase_C1A_papain-like.
DR InterPro; IPR013201; Prot_inhib_I29.
DR Pfam; PF08246; Inhibitor_I29; 1.
DR Pfam; PF00112; Peptidase_C1; 1.
DR PRINTS; PR00705; PAPAIN.
DR SMART; SM00848; Inhibitor_I29; 1.
DR SMART; SM00645; Pept_C1; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Protease; Reference proteome; Signal; Thiol protease; Vacuole.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..141
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q7F3A8"
FT /id="PRO_0000455738"
FT CHAIN 142..378
FT /note="Cysteine endopeptidase RepA"
FT /id="PRO_5013421037"
FT ACT_SITE 167
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10088"
FT ACT_SITE 303
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10089"
FT ACT_SITE 324
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10090"
SQ SEQUENCE 378 AA; 40933 MW; 33434AFA66B349BF CRC64;
MLRCFLVAAA AVALAAAAAA PARAIPFTES DLSSEESLRA LYERWRSRYT VSRPAASGGV
GNDDGEARRR FNVFVENARY IHEANRRGGR PFRLALNKFA DMTTDEFRRT YAGSRARHHR
SLSGGRGGEG GSFRYGGDDE DNLPPAVDWR ERGAVTGIKD QGQCGSCWAF STVAAVEGVN
KIKTGRLVTL SEQELVDCDT GDNQGCDGGL MDYAFQFIKR NGGITTESNY PYRAEQGRCN
KAKASSHDVT IDGYEDVPAN DESALQKAVA NQPVAVAVEA SGQDFQFYSE GVFTGECGTD
LDHGVAAVGY GITRDGTKYW IVKNSWGEDW GERGYIRMQR GVSSDSNGLC GIAMEASYPV
KSGARNAAAS NRVVKDEM
