ID   REPA_PASVK              Reviewed;         323 AA.
AC   Q00338;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Replication-associated protein A;
DE            Short=RepA;
DE            EC=3.1.21.-;
GN   ORFNames=C1;
OS   Panicum streak virus (isolate Kenya) (PanSV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Mastrevirus.
OX   NCBI_TaxID=268780;
OH   NCBI_TaxID=59788; Megathyrsus maximus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1588314; DOI=10.1099/0022-1317-73-5-1041;
RA   Briddon R.W., Lunness P., Chamberlain L.C., Brundish H., Pinner M.S.,
RA   Markham P.G.;
RT   "The nucleotide sequence of an infectious insect-transmissible clone of the
RT   geminivirus Panicum streak virus.";
RL   J. Gen. Virol. 73:1041-1047(1992).
CC   -!- FUNCTION: Implicated in enhancement of V-sense gene expression. Acts a
CC       an inhibitor of C-sense gene transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homooligomer. Interacts (via LXCXE domain) with host
CC       retinoblastoma-related protein 1 (RBR1), and may thereby deregulate the
CC       host cell cycle. Part of the C- and V-complexes which are RepA-Rep-DNA
CC       complexes involved in the c-sense and v-sense transcription (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=RepA;
CC         IsoId=Q00338-1; Sequence=Displayed;
CC       Name=Rep;
CC         IsoId=P0C647-1; Sequence=External;
CC   -!- DOMAIN: There are 3 rolling circle replication (RCR) motifs. RCR-2 may
CC       be involved in metal coordination. RCR-3 is required for phosphodiester
CC       bond cleavage for initiation of RCR.
CC   -!- DOMAIN: The LXCXE motif specifically binds to host RBR1. {ECO:0000250}.
CC   -!- MISCELLANEOUS: [Isoform RepA]: Produced from the unspliced transcript.
CC   -!- SIMILARITY: Belongs to the geminiviridae Rep protein family.
CC       {ECO:0000305}.
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DR   EMBL; X60168; CAA42735.1; -; Genomic_DNA.
DR   PIR; JQ1552; JQ1552.
DR   SMR; Q00338; -.
DR   PRIDE; Q00338; -.
DR   Proteomes; UP000007896; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016888; F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
DR   InterPro; IPR001146; Gemini_AL1_MSV.
DR   InterPro; IPR001191; Gemini_AL1_REP.
DR   InterPro; IPR022690; Gemini_AL1_REP_cat-dom.
DR   InterPro; IPR022692; Gemini_AL1_REP_central.
DR   Pfam; PF00799; Gemini_AL1; 1.
DR   Pfam; PF08283; Gemini_AL1_M; 1.
DR   PRINTS; PR00227; GEMCOATAL1.
DR   PRINTS; PR00229; GEMCOATMSVL1.
PE   3: Inferred from homology;
KW   Activator; Alternative splicing; DNA-binding; Endonuclease;
KW   G1/S host cell cycle checkpoint dysregulation by virus; Host cytoplasm;
KW   Host nucleus; Host-virus interaction; Hydrolase; Metal-binding;
KW   Modulation of host cell cycle by virus; Nuclease; Repressor.
FT   CHAIN           1..323
FT                   /note="Replication-associated protein A"
FT                   /id="PRO_0000222209"
FT   REGION          181..193
FT                   /note="Oligomerization"
FT                   /evidence="ECO:0000250"
FT   REGION          256..286
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           25..29
FT                   /note="RCR-1"
FT   MOTIF           67..72
FT                   /note="RCR-2"
FT   MOTIF           107..110
FT                   /note="RCR-3"
FT   MOTIF           204..208
FT                   /note="LXCXE motif, interaction with host RBR1"
FT   ACT_SITE        107
FT                   /note="For DNA cleavage activity"
FT                   /evidence="ECO:0000250"
FT   BINDING         59
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         67
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         69
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   323 AA;  37027 MW;  60418182D167D903 CRC64;
     MSTVGSSSEG RHSVRCFRHR NANTFLTYSK CPLEPEFIGE HLFRLTREYE PAYILVVRET
     HTDGTWHCHA LLQCIKPCTT RDERYFDIDR YHGNIQSAKS TDKVREYILK DPKDKWEKGT
     YIPRKKSFVP PGKEPAEKKP TKDEVMREIM THATSREEYL SLVQSSLPYD WATKLNYFEY
     SASRLFPDIA EPYTNPHPTT EYDLHCNETI EDWLKPNIYQ VSPQAYKLLE PSCLSLEQAI
     ADLEWLDDTT RMLQEKEREA STSAAQHGQV KHPGLEASDD TTTGKTISTG LHMMKKLSTM
     SLTTFPSSSV RAGNDSSAAK KTT