ID   REPB_ACIFR              Reviewed;         352 AA.
AC   P27186;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=DNA primase;
DE            EC=2.7.7.-;
DE   AltName: Full=Replication primase;
GN   Name=repB;
OS   Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans).
OG   Plasmid pTF-FC2.
OC   Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales;
OC   Acidithiobacillaceae; Acidithiobacillus.
OX   NCBI_TaxID=920;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1761233; DOI=10.1016/0378-1119(91)90481-p;
RA   Dorrington R.A., Bardien S., Rawlings D.E.;
RT   "The broad-host-range plasmid pTF-FC2 requires a primase-like protein for
RT   autonomous replication in Escherichia coli.";
RL   Gene 108:7-14(1991).
CC   -!- FUNCTION: Functions as a primase with respect to replication at the
CC       (vegetative) origin of replication of pTF-FC2.
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DR   EMBL; M64981; AAA27386.1; -; Genomic_DNA.
DR   PIR; JS0637; JS0637.
DR   AlphaFoldDB; P27186; -.
DR   SMR; P27186; -.
DR   PRIDE; P27186; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR039459; RepB-like_DNA_primase_dom.
DR   Pfam; PF16793; RepB_primase; 1.
PE   4: Predicted;
KW   DNA replication; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Plasmid; Transcription; Transferase.
FT   CHAIN           1..352
FT                   /note="DNA primase"
FT                   /id="PRO_0000180533"
FT   REGION          145..172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          318..352
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        157..172
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        319..342
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   352 AA;  40156 MW;  6B5E63D06FA59E24 CRC64;
     MKSEQLKQFE RYAEAVGAER YRVTSIKMQA DGRKQTFILD KKDGITRGFT PQEIEQRTPE
     MQRLQRRGEN LYYTPLSDKK HHILIDDMNR EKLERLIKDG YQPAAVLESS PGNYQAIITV
     SKLGTAHDKD VGNRLSDALN REYGDPKLSG AIHPHRAPGY ENRKPKHQRE DGSYPEVRLL
     KAERRECVKA LALSSQIDAE YQRQAALKAQ QPERTKAKPA LELAAASGSA IDAYQRHYRD
     VLKRQRGGEV DLSRVDSMIA VRMRVTGHDQ AAIEGAIRQC APATRQKDEG RDWNDYAQRT
     ARYAYSAAQP QAADLGKYRQ QWEKLEGREP VRQQEQAKAQ KIERDNSPGM SL