ID   REPB_ECOLI              Reviewed;         325 AA.
AC   Q57154; Q60231;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=RepFIB replication protein A;
GN   Name=repB; Synonyms=repA; OrderedLocusNames=ECOK12F033;
OS   Escherichia coli (strain K12).
OG   Plasmid F, Plasmid IncFI P307, Plasmid IncFI pHH502, and
OG   Plasmid IncFI ColVBtrp.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63; PLASMID=F;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFI ColVBtrp, and IncFI pHH502;
RX   PubMed=8356112; DOI=10.1006/plas.1993.1020;
RA   Gibbs M.D., Spiers A.J., Bergquist P.L.;
RT   "RepFIB: a basic replicon of large plasmids.";
RL   Plasmid 29:165-179(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFI P307;
RX   PubMed=2651415; DOI=10.1128/jb.171.5.2697-2707.1989;
RA   Saul D., Spiers A.J., McAnulty J., Gibbs M.G., Bergquist P.L., Hill D.F.;
RT   "Nucleotide sequence and replication characteristics of RepFIB, a basic
RT   replicon of IncF plasmids.";
RL   J. Bacteriol. 171:2697-2707(1989).
RN   [4]
RP   EXPRESSION REGULATION, AND DNA REPEAT ELEMENT BINDING.
RX   PubMed=8320218; DOI=10.1128/jb.175.13.4016-4024.1993;
RA   Spiers A.J., Bhana N., Bergquist P.L.;
RT   "Regulatory interactions between RepA, an essential replication protein,
RT   and the DNA repeats of RepFIB from plasmid P307.";
RL   J. Bacteriol. 175:4016-4024(1993).
CC   -!- FUNCTION: This protein is essential for plasmid replication; it is
CC       involved in copy control functions. In vitro, binds to the DNA repeat
CC       units, BCDD'D'', EFG and HIJ.
CC   -!- SIMILARITY: Belongs to the initiator RepB protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB61762.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AP001918; BAA97903.1; -; Genomic_DNA.
DR   EMBL; L01255; AAA71885.1; -; Unassigned_DNA.
DR   EMBL; L01256; AAA71886.1; -; Unassigned_DNA.
DR   EMBL; M26308; AAB61762.1; ALT_INIT; Genomic_DNA.
DR   PIR; I40829; I40829.
DR   RefSeq; NP_061412.1; NC_002483.1.
DR   RefSeq; WP_000361610.1; NZ_STEB01000058.1.
DR   AlphaFoldDB; Q57154; -.
DR   PRIDE; Q57154; -.
DR   PRO; PR:Q57154; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000525; Initiator_Rep_prot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF01051; Rep_3; 1.
PE   1: Evidence at protein level;
KW   DNA replication; DNA-binding; Plasmid; Plasmid copy control.
FT   CHAIN           1..325
FT                   /note="RepFIB replication protein A"
FT                   /id="PRO_0000068302"
FT   REGION          279..298
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         60
FT                   /note="A -> D (in copy number mutant)"
SQ   SEQUENCE   325 AA;  37284 MW;  B7A4B637B18997DB CRC64;
     MDKSSGELVT LTPNNNNTVQ PVALMRLGVF VPTLKSLKNS KKNTLSRTDA TEELTRLSLA
     RAEGFDKVEI TGPRLDMDND FKTWVGIIHS FARHNVIGDK VELPFVEFAK LCGIPSSQSS
     RRLRERISPS LKRIAGTVIS FSRTDEKHTR EYITHLVQSA YYDTERDIVQ LQADPRLFEL
     YQFDRKVLLQ LKAINALKRR ESAQALYTFI ESLPRDPAPI SLARLRARLN LKSPVFSQNQ
     TVRRAMEQLR EIGYLDYTEI QRGRTKFFCI HYRRPRLKAP NDESKENPLP PSPAEKVSPE
     MAEKLALLEK LGITLDDLEK LFKSR